Abstract: The present experiment was conducted to obtain and analyzed cDNA sequence of β-actin gene from Paramisgurnus dabryanus, and to study the tissue expression characterization of β-actin mRNA in P. dabryanus. The complete β-actin cDNA sequences were obtained from the P. dabryanus muscle tissue using RT-PCR and RACE methods. The complete β-actin cDNA of P. dabryanus was 1710 bp in length, containing an open reading frame of 1128bp (encoding 375 amino acids), flanked by 73 bp 5'UTR and 509 bp 3'UTR. Sequence analysis indicated that the P. dabryanus β-actin exhibits more than 98% amino acid identity and 87% nucleotide identity with the β-actin of other fishes. Phylogenetic assay indicated that the β-actin gene of P. dabryanus was highly conserved during evolution. Results from quantitative real-time PCR (qRT-PCR) showed the β-actin gene is widely expressed in different organs including skeletal muscle, heart, liver, spleen, intestine, stomach, testis and ovary. The result provided sequence information not only for analyzing gene expression of P. dabryanus using β-actin gene as internal control, but for molecular systematic research of P. dabryanus.