Abstract: In order to rationally apply nitrogen fertilizer and provide theoretical basis for efficient utilization of nitrogen fertilizer, the optimum ratio of base-tiller-ear fertilizer of rice was studied. The study designed 5 ratios of base-tiller-ear fertilizer with the same total nitrogen amount and analyzed the nitrogen absorption and utilization of rice. The results showed that the nitrogen harvest index showed a downward trend with the increase of ear fertilizer. Nitrogen translocation amount, nitrogen translocation efficiency, nitrogen translocation contribution rate of rice leaves and stems, nitrogen physiological efficiency, nitrogen efficiency, nitrogen uptake efficiency, nitrogen use efficiency, nitrogen behavior use efficiency, nitrogen agronomic use efficiency, nitrogen physiological use efficiency, nitrogen partial productivity and soil nitrogen dependency rate of rice increased firstly and then decreased, and reached the maximum at 6:4. Grain yield showed a significantly positive correlation with the nitrogen translocation contribution rate, the nitrogen efficiency and the nitrogen agronomic use efficiency, and showed a positive correlation with the nitrogen translocation efficiency and the nitrogen physiological use efficiency; the spike number showed a significantly positive correlation with the nitrogen harvest index. Glumous flower number of per spike showed a positive correlation with the nitrogen translocation efficiency, and showed a negative correlation with the nitrogen partial productivity. Thousand seed weight showed a negative correlation with the nitrogen physiological use efficiency and showed a significant positive correlation with the nitrogen partial productivity. Therefore, the optimum ratio of base-tiller fertilizer was 6:4, which could achieve the purpose of optimizing the group and obtaining high yield.