Abstract: In order to improve the research on embryonic development of Callosobruchus maculatus, provide a scientific theoretical basis for effective management and offer reliable and accurate experimental data on prevention and control of C. maculatus for China’s grain agency, embryonic development process of quarantine pest C. maculatus was studied at the temperature of 28℃ and relative humidity of 70% under microscopic observation. Then, the effect of temperature on embryonic development duration was analyzed. The results showed that the embryonic development duration of C. maculatus lasted 118 h. Embryonic development process mainly included five stages which were cleavage, formation of blastoderm and germ band, formation of germinal layer and membrane, segmentation of embryonic body and formation of appendages, dorsal enclosure and the mature of embryo; and the corresponding time was 0-11 h, 12-16 h, 17-21 h, 22-90 h and 91-118 h. Incubation period of C. maculatus egg decreased with the increase of temperature at the range of 20-37℃. This study provided a reference for the classification and phylogeny of C. maculatus, and laid a foundation for the further molecular biological study of C. maculatus.