Abstract: The paper aims at selenium bio-concentration and translocation inAstragalus membranaceus . To study the dry matter weight of roots and shoots, selenium content and translocation inA. membranaceus under different concentrations (0, 5, 10, 50, 100 μmol/L) of sodium selenate and sodium selenite, root application and foliage spray were used in the experiment. The results showed that both sodium selenate and sodium selenite were beneficial to the increase of dry matter weight of roots and shoots and selenium content. With the increase of selenium concentration, the dry matter weight showed a trend of first increase then decrease, the selenium content of shoots showed a trend of rise, while that of the roots was the opposite. Adding sodium selenate and sodium selenite in nutrient solution, the dry matter weight in roots and shoots under 10 μmol/L Se concentration increased by 49.89%, 60.87% and 23.36%, 60.00%, respectively, compared with those of CK. Moreover, Se content in roots and shoots increased more in sodium selenate treatments than that in sodium selenite treatments under the same Se concentration, Se content of shoots reached the maximum of 119.13 μg/g in 100 μmol/L treatment and that of roots reached the maximum of 52.25 μg/g in 5 μmol/L treatment. The root application of sodium selenite was more conducive to the enrichment of Se, moreover, the selenium translocation ability from roots to shoots increased with the selenium application concentration.