Abstract: To select suitable for sugar beet varieties purity identification of DAMD primers, respectively using 12 different sugar beet varieties of 26 DAMD primers for amplification, and 6% respectively using polyacrylamide gel electrophoresis and 2% agarose gel electrophoresis, respectively, to test the amplification products.Results from 26 DAMD article 16 primers screened in clear primer amplification, this article 16 primers amplification out 139 bands, including polymorphism bands for 122, the percentage of polymorphic (87.7%), the 16 primers can be used as a sugar beet varieties purity and authenticity identification of core primers, also found that the polyacrylamide gel electrophoresis and agarose gel electrophoresis results difference is not big, but the agarose gel is easy to make, test the advantages of simple and does not apply to any potions, recommend beet DAMD amplification product detection using agarose gel electrophoresis.