Stress Tolerance Improvement by BvM14-CCoAOMT Gene in Sugar Beet M14 Strain

doi:10.11924/j.issn.1000-6850.casb18060037

Abstract

Abstract: To study the function of caffeoyl CoA O-methyltransferase gene (BvM14-CCoAOMT) in sugar beet M14 strain, the transgenic plants of T1 generation were obtained by the method of leaf disc method, and the resistance of positive transgenic plants was analyzed. Furthermore, compared with wild type and tramsformed empty carrier plants, the resistance of positive transgenic plants were analyzed under the conditions of control, 100 mmol/L NaCl, 200 mmol/L NaCl, 100 mmol/L mannitol and 200 mmol/L mannitol. It was found that under 100 mmol/L NaCl and 200 mmol/L NaCl, the fresh weight, lignin content and chlorophyll content of the transgenic plants were significantly higher than those of control plants. In addition, the results showed that the root length and chlorophyll content of transgenic plants under 100 mmol/L mannitol were significantly higher than those of the control plants. For example, the root length and chlorophyll content of transgenic plants were 0.68 cm and 0.31 mg respectively, while the root length and chlorophyll content of wild type plants were 0.51 cm and 0.18 mg respectively. The results showed that transgenic tobacco plants with BvM14-CCoAOMT gene had strong resistance to salt and drought stress, and provide the theoretical basis for the further study of BvM14-CCoAOMT function.

CLC Number:

S566.3

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