Analysis of Transicriptomes and Differential Expression in Bothriochloa ischaemum

doi:10.11924/j.issn.1000-6850.casb18060105

Abstract

Abstract: In order to obtain the transcriptome database and differentially expressed genes of Bothriochloa ischaemum, transcriptome sequencing of the control group and drought stress group was performed with Illumina HiSeq 4000 high-throughput sequencing technology. A total of 118580 unigenes were obtained from the assembly analysis. All unigenes were compared with the gene database, and the functional annotations were performed on unigenes, about 50070 unigenes were got. According to the function of GO, unigenes in the transcriptome of B. ischaemum could be roughly divided into three categories: cellular component, molecular function and biological process, with a total of 54 branches. All unigenes were compared with the KOG database; there were 1586 unigenes which were classi fi ed to 25 terms. Using the KEGG database as a reference, unigenes could be mapped to 116 classes depending on the metabolic pathway. Through the differentially expressed analysis, we got 3987 differentially expressed genes, the up-regulated genes accounted for 39.45%, the down-regulated genes accounted for 60.55%. This study provides a valuable genome data source for molecular biology research of B. ischaemum.

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