The Optimal Condition for Callus Induction and Redifferentiation of Panicum miliaceum L.

doi:10.11924/j.issn.1000-6850.casb18120078

Abstract

Abstract:

The aim is to explore the optimum condition for callus induction and redifferentiation by tissue culture technology, so as to lay a foundation for the establishment of genetic transformation system of Panicum miliaceum L.. Five Panicum miliaceum L. varieties were used to study the optimal condition for callus induction and redifferentiation. Plant hormone 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to induce shoot tip callus. Varieties with high germination rate, high callus induction rate and low bacterial infection rate were screened. The optimum hormone concentration and ratio for inducing callus were identified. The results showed that ‘Jishu No. 2’ had high germination and callus induction rates and low bacterial infection rate, showing it was suitable for tissue culture. 2,4-D had the best induction effect on ‘Jishu No. 2’, with the optimum concentration of 2.5 mg/L and the induction rate of 86.67%. The callus was light yellow block, compact in structure and hard in texture. The embryogenic callus formed after subculture was better than that of other varieties. In the process of redifferentiation, when the culture condition was 2.5 mg/L 2,4-D + 3.5 mg/L TDZ, tender buds appeared obviously. The optimum condition for callus induction and redifferentiation of ‘Jishu No. 2’ are obtained, which could provide reference for constructing the regeneration system of Panicum miliaceum L..

Key words: Panicum miliaceum, callus induction, redifferentiation, shoot tip, plant hormones, tissue culture

CLC Number:

S516

Chunfang Wang, Jingjing Liu, Wei Li, Surong Luan, Shenkui Shi, Guoqing Liu. The Optimal Condition for Callus Induction and Redifferentiation of Panicum miliaceum L.[J]. Chinese Agricultural Science Bulletin, 2020, 36(9): 94-99.

Figures/Tables 5

References 28

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名称	发芽率/%	愈伤组织诱导率/%	愈伤生长状况	染菌率/%
冀黍1号	80.00	55.00	淡黄色颗粒状	2.80
冀黍2号	92.00	89.00	淡黄色块状,结构紧密,质地较硬	1.00
龙黍18	78.00	71.00	淡黄色块状,质地松软	2.10
龙黍21	83.00	80.00	白色块状,质地紧密	3.33
龙黍23	88.00	37.00	淡黄色块状,结构紧密,质地较硬	4.20

名称	发芽率/%	愈伤组织诱导率/%	愈伤生长状况	染菌率/%
冀黍1号	80.00	55.00	淡黄色颗粒状	2.80
冀黍2号	92.00	89.00	淡黄色块状,结构紧密,质地较硬	1.00
龙黍18	78.00	71.00	淡黄色块状,质地松软	2.10
龙黍21	83.00	80.00	白色块状,质地紧密	3.33
龙黍23	88.00	37.00	淡黄色块状,结构紧密,质地较硬	4.20

处理	激素配比	处理	激素配比
T1	2,4-D	T4	2,4-D+ZT (1:1)
T2	NAA	T5	2,4-D+TDZ (1:1)
T3	ZT	T6	NAA+6BA (1:1)

处理	激素配比	处理	激素配比
T1	2,4-D	T4	2,4-D+ZT (1:1)
T2	NAA	T5	2,4-D+TDZ (1:1)
T3	ZT	T6	NAA+6BA (1:1)

处理	浓度/(mg/L)	愈伤生长量	愈伤生长状况	愈伤组织诱导率/%	生根率/%	染菌率/%
2,4D	0.5	+++	黄绿色颗粒状,结构致密	46.67	—	—
	1.0	+++	黄绿色颗粒状,结构较致密	50	—	—
	1.5	+++	黄绿色块状,结构致密	60	—	—
	2.0	+++	绿色和暗黄色块状,结构较致密	68.33	—	—
	2.5	++++	淡绿色块状,结构致密	86.67	—	—
	3.0	+++	黄绿色和红色块状,结构致密	54.67	—	—
NAA	0.5	++	白色小颗粒状,结构疏松,生根	43.67	26.33	—
	1.0	+++	白色小颗粒状,结构疏松,生根	53.00	33.00	—
	1.5	+++	黄绿色分散状,愈伤较大,生根	93.00	53.67	—
	2.0	++++	淡黄色分散状,结构疏松,生根	80.67	43.00	—
	2.5	+++	绿色和暗黄色块状,结构较致密	88.00	98.00	—
2,4-D+TDZ	0.5	++	白色小颗粒状,结构疏松	12.33	—	0.00
	1.0	++	白色小颗粒状,结构疏松	10.33	—	5.67
	1.5	++	黄绿色分块状,愈伤较大,	45.00	—	13.00
	2.0	++	淡黄色块状,结构紧密,叶片生长	21.00	—	14.33
	2.5	++	绿色和暗黄色块状,结构较致密,有叶片生长	18.33	—	8.00
	3.0	++	绿黄色块状,结构较致密,有部分褐化	25.67	—	14.00