Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (13): 89-93.doi: 10.11924/j.issn.1000-6850.casb19030046
Special Issue: 生物技术
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Li Huiling1, Luo Yulan2, Zhang Zhang1(), Yin Lijuan2, Li Pujin2, Zhang Dongmei2()
Received:
2019-03-13
Revised:
2019-06-03
Online:
2020-05-05
Published:
2020-04-16
Contact:
Zhang Zhang,Dongmei Zhang
E-mail:zhangzhang@jala.com.cn;418517920@qq.com
CLC Number:
Li Huiling, Luo Yulan, Zhang Zhang, Yin Lijuan, Li Pujin, Zhang Dongmei. Study on the Seed Germination and Tissue Culture of Rosa graciliflora[J]. Chinese Agricultural Science Bulletin, 2020, 36(13): 89-93.
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URL: https://www.casb.org.cn/EN/10.11924/j.issn.1000-6850.casb19030046
序号 | 10%次氯酸钠溶液处理时间/min | 0.05%升汞处理时间/min | 接种数/个 | 污染率/% | 消毒过度死亡数/个 | 腋芽诱导数/个 | 腋芽诱导率/% |
---|---|---|---|---|---|---|---|
1 | 10 | 5 | 30 | 63.3 | 2 | 9 | 30.0 |
2 | 10 | 10 | 30 | 26.7 | 3 | 19 | 63.3 |
3 | 10 | 15 | 30 | 20.0 | 8 | 16 | 53.3 |
4 | 15 | 5 | 30 | 53.3 | 4 | 10 | 33.3 |
5 | 15 | 10 | 30 | 20.0 | 4 | 20 | 66.7 |
6 | 15 | 15 | 30 | 6.7 | 21 | 7 | 23.3 |
序号 | 10%次氯酸钠溶液处理时间/min | 0.05%升汞处理时间/min | 接种数/个 | 污染率/% | 消毒过度死亡数/个 | 腋芽诱导数/个 | 腋芽诱导率/% |
---|---|---|---|---|---|---|---|
1 | 10 | 5 | 30 | 63.3 | 2 | 9 | 30.0 |
2 | 10 | 10 | 30 | 26.7 | 3 | 19 | 63.3 |
3 | 10 | 15 | 30 | 20.0 | 8 | 16 | 53.3 |
4 | 15 | 5 | 30 | 53.3 | 4 | 10 | 33.3 |
5 | 15 | 10 | 30 | 20.0 | 4 | 20 | 66.7 |
6 | 15 | 15 | 30 | 6.7 | 21 | 7 | 23.3 |
序号 | NAA浓度/(mg/L) | 6-BA浓度/(mg/L) | KT浓度/(mg/L) | 平均增殖系数 | 芽苗长势 |
---|---|---|---|---|---|
1 | 0.2 | 1.0 | 0 | 0.5 | 丛生芽生长慢,叶色绿 |
2 | 0.2 | 1.5 | 0 | 1.4 | 丛生芽生长快,叶色绿 |
3 | 0.2 | 2.0 | 0 | 2.1 | 丛生芽生长快,叶色绿 |
4 | 0.2 | 3.0 | 0 | 0.2 | 丛生芽生长快,叶色黄绿 |
5 | 0 | 2.0 | 0.2 | 0 | 丛生芽生长慢,叶缘发黄 |
6 | 0 | 2.0 | 0.5 | 0 | 丛生芽生长慢,叶缘发黄 |
序号 | NAA浓度/(mg/L) | 6-BA浓度/(mg/L) | KT浓度/(mg/L) | 平均增殖系数 | 芽苗长势 |
---|---|---|---|---|---|
1 | 0.2 | 1.0 | 0 | 0.5 | 丛生芽生长慢,叶色绿 |
2 | 0.2 | 1.5 | 0 | 1.4 | 丛生芽生长快,叶色绿 |
3 | 0.2 | 2.0 | 0 | 2.1 | 丛生芽生长快,叶色绿 |
4 | 0.2 | 3.0 | 0 | 0.2 | 丛生芽生长快,叶色黄绿 |
5 | 0 | 2.0 | 0.2 | 0 | 丛生芽生长慢,叶缘发黄 |
6 | 0 | 2.0 | 0.5 | 0 | 丛生芽生长慢,叶缘发黄 |
序号 | 基础培养基 | NAA浓度/(mg/L) | 6-BA浓度/(mg/L) | IBA浓度/(mg/L) | 生根率 | 生根情况及组培苗长势 |
---|---|---|---|---|---|---|
1 | 1/2MS | 0.1 | 0.2 | 0 | 0 | 不生根,易出愈伤组织 |
2 | 1/2MS | 0.3 | 0 | 2.0 | 0 | 不生根,叶枯黄,萎凋死亡 |
3 | 1/2MS | 0.3 | 0 | 0.1 | 0 | 不生根,叶枯黄 |
4 | 1/2MS | 0 | 0 | 0.1 | 0 | 不生根,叶色绿 |
5 | 1/2MS | 0 | 0 | 0.3 | 0 | 不生根,叶色绿 |
6 | 1/2MS | 0 | 0 | 0.5 | 0 | 不生根,叶色绿 |
7 | WPM | 0 | 0 | 0.1 | 65% | 生根0~3条,生根慢,叶色绿 |
8 | WPM | 0 | 0 | 0.3 | 90% | 生根0~9条,生根快,叶色绿 |
9 | WPM | 0 | 0 | 0.5 | 95% | 生根0~4条,生根快,叶色黄绿 |
序号 | 基础培养基 | NAA浓度/(mg/L) | 6-BA浓度/(mg/L) | IBA浓度/(mg/L) | 生根率 | 生根情况及组培苗长势 |
---|---|---|---|---|---|---|
1 | 1/2MS | 0.1 | 0.2 | 0 | 0 | 不生根,易出愈伤组织 |
2 | 1/2MS | 0.3 | 0 | 2.0 | 0 | 不生根,叶枯黄,萎凋死亡 |
3 | 1/2MS | 0.3 | 0 | 0.1 | 0 | 不生根,叶枯黄 |
4 | 1/2MS | 0 | 0 | 0.1 | 0 | 不生根,叶色绿 |
5 | 1/2MS | 0 | 0 | 0.3 | 0 | 不生根,叶色绿 |
6 | 1/2MS | 0 | 0 | 0.5 | 0 | 不生根,叶色绿 |
7 | WPM | 0 | 0 | 0.1 | 65% | 生根0~3条,生根慢,叶色绿 |
8 | WPM | 0 | 0 | 0.3 | 90% | 生根0~9条,生根快,叶色绿 |
9 | WPM | 0 | 0 | 0.5 | 95% | 生根0~4条,生根快,叶色黄绿 |
[1] | 隋云吉, 郭润华 . 不同处理对3种蔷薇种子萌发的影响[J]. 种子, 2015,34(5):86-91. |
[2] | 中国科学院中国植物志编委会. 中国植物志·第37卷[M]. 北京: 科学出版社, 1985: 380. |
[3] | 朱小虎, 王晴, 程世平 . 野蔷薇种子休眠机理研究[J]. 山东农业科学, 2013,45(10):59-62. |
[4] | 李增武, 钟玲, 赵梁军 . 野蔷薇种子休眠和萌发整齐度研究[J]. 种子, 2011,30(10):42-44. |
[5] | 于非, 王禹, 张毓 . 月季组织培养快繁体系的建立[J]. 中国林副特产, 2017,4(2):24-25. |
[6] | 薛其勤, 李美芹, 裴华丽 , 等. ‘鲁硕红’蔷薇组织培养快繁体系的建立[A]. 第四届中华农圣文化国际研讨会论文集:弘扬世界农业文明发展现代绿色农业[C]. 北京: 中国农业科技出版社, 2013: 387-395. |
[7] | 李剑宁 . 粉团蔷薇组织培养技术研究[J]. 甘肃林业, 2013,4:38-39. |
[8] | 杨立新, 高英, 范彦晓 . 蔷薇属植物护肤功效的民族植物学研究与考证[J]. 中药学, 2018,41(2):498-502. |
[9] | 王艳, 谢蓉, 蔡丹燕 , 等. 金樱根、茎炮制品抗菌抗炎作用研究[J]. 中药学, 2014,37(8):1356-1359. |
[10] | Jiang H, Han H, Man W J , et al. Ursane-type triterpenoids from the roots of Rosa multiflora with their anti-inflammatory activity[J]. Journal of asian natural products research, 2018,12(10):1-7. |
[11] | Inés Mármol, Cristina, Sánchez-de-Diego, Nerea Jiménez-Moreno , et al. Therapeutic Applications of Rose Hips from Different Rosa Species[J]. International Journal of Molecular Sciences, 2017,18:1137. |
[12] | Kwan Hee Park, Mi Sook Jeong, Kwang Jun Park , et al. Topical Application of Rosa multiflora Root Extract Improves Atopic Dermatitis-Like Skin Lesions Induced by Mite Antigen in NC/Nga Mice[J]. Biological and Pharmaceutical Bulletin, 2014,37(1) 178-183. |
[13] | Phetcharat L, Wongsuphasawat K, Winther K . The effectiveness of a standardized rose hip powder, containing seeds and shells of Rosa canina, on cell longevity, skin wrinkles, moisture, and elasticity[J]. Clinical Interventions in Aging. 2015,10:1849-1856. |
[14] | Ayati Zahra, Amiri Mohammad S, Ramezani Mahin , et al. Phytochemistry, Traditional Uses and Pharmacological Profile of Rose Hip: A Review[J]. Bentham Science Publishers, 2018,24(35):4101-4124. |
[15] | Jeon Ji-Hyeon, Kim Seung-Chul . Comparative Analysis of the Complete Chloroplast Genome Sequences of Three Closely Related East-Asian Wild Roses (Rosa sect. Synstylae; Rosaceae)[J]. Genes, 2019,10(23). |
[16] | Jian Hongying, Zhang Yonghong, Qiu Xianqin , et al. Yalongjiang River Has Had an Important Role in the Dispersal and Divergence of Rosa soulieana in the Hengduan Mountains of China[J]. Plos One, 2016,11(6):e0158586. |
[17] | Marie Fouge`re-Danezan, Simon Joly, Anne Bruneau , et al. Phylogeny and biogeography of wild roses with specific attention to polyploids[J]. Annals of Botany, 2015,115:275-291. |
[18] | Friederike Gallenmüller, Amélie Feus, Kathrin Fiedler , et al. Rose Prickles and Asparagus Spines - Different Hook Structures as Attachment Devices in Climbing Plants[J]. Plos One, 2015,10(12):e0143850. |
[19] | Han Yu, Tang Aoying, Wan Huihua , et al. An APETALA2 Homolog, RcAP2, Regulates the Number of Rose Petals Derived From Stamens and Response to Temperature Fluctuations[J]. Frontiers in Plant Science, 2018,9:481. |
[20] | Feng Liguo, Ding Han, Wang Jia , et al. Molecular cloning and expression analysis of RrNHX1 and RrVHA-c genes related to salt tolerance in wild Rosa rugosa[J]. Saudi Journal of Biological Sciences, 2015,22:417-423. |
[21] | Li Shubin, Zhong Micai, Dong Xue , et al. Comparative transcriptomics identifies patterns of selection in roses[J]. BMC Plant Biology, 2018,18:371. |
[22] | Noriko Nakamura, Hideki Hirakawa, Shusei Sato , et al. Genome structure of Rosa multiflora, a wild ancestor of cultivated roses[J]. DNA Research, 2018,25(2):113-121. |
[23] | 杨逢玉, 杨帆, 郭润华 . 引种条件下八种新疆野生蔷薇种子自然萌发规律[J]. 北方园艺, 2017(13):108-112. |
[24] | 王世平 . 干湿交替处理对刺玫蔷薇种子萌发的影响[J]. 现代园艺, 2018(3):17-18. |
[25] | 陈睿, 吴洪娥, 周艳 , 等. 7种蔷薇属植物种子的发芽试验研究[J]. 种子, 2013,32(3):72-74. |
[26] | 刘小菊, 周建会 . 不同试剂解除天山野生蔷薇种子休眠的效果[J]. 贵州农业科学, 2018,46(1):74-76. |
[27] | Ding Xiao-Liu, Xu Ting-Liang, Wang Jing , et al. Distribution of 45S rDNA in Modern Rose Cultivars (Rosa hybrida), Rosa rugosa, and Their Interspecific Hybrids Revealed by Fluorescence in situ Hybridization[J]. Cytogenetic and Genome Research, 2016,149(3):226-235. |
[28] | 徐立军, 李志斌, 蒋淑磊 , 等. 大马士革玫瑰组织培养技术研究[J]. 河北林业科技, 2015,2:19-21. |
[29] | 于非, 王禹, 张毓 . 月季组织培养快繁体系的建立[J]. 中国林副特产, 2017,2:24-25. |
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