Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2020, Vol. 36 ›› Issue (13): 89-93.doi: 10.11924/j.issn.1000-6850.casb19030046

Special Issue: 生物技术

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Study on the Seed Germination and Tissue Culture of Rosa graciliflora

Li Huiling1, Luo Yulan2, Zhang Zhang1(), Yin Lijuan2, Li Pujin2, Zhang Dongmei2()   

  1. 1 JALA Research Center, JALA Group Co., Ltd, Shanghai 200233
    2 Shanghai Landscape Gardening Research Institute, Shanghai 200232
  • Received:2019-03-13 Revised:2019-06-03 Online:2020-05-05 Published:2020-04-16
  • Contact: Zhang Zhang,Dongmei Zhang E-mail:zhangzhang@jala.com.cn;418517920@qq.com

Abstract:

This research aims to obtain a complete plant of Rosa graciliflora and establish its rapid propagation system. Germination rate of R. graciliflora seed was observed on different cold stratification time. Meanwhile, tender stem segments were used as explants to investigate the effects of different sterilized reagents, sterilized time and phytohormone combinations on the axillary bud induction and proliferation rate, and also rooting rate. Furthermore, the culture medium was optimized for each growth stage. The results showed that the seed germination rate was as high as 35% when treated by sand storage in low temperature for 9 months. The optimum combination for stem sterilization was 10% sodium hypochlorite solution for 15 min followed by 0.05% mercuric chloride solution for 10 min, and the optimal medium for bud proliferation was 1/2MS+NAA 0.2 mg/L+6-BA 2.0 mg/L. The rooting rate reached 90% in the rooting medium of WPM+IBA 0.3 mg/L. In conclusion, this research explores the seed germination condition of R. graciliflora and establishes its rapid propagation system, which could lay a foundation for the industrial production and further utilization of R. graciliflora.

Key words: Rosa graciliflora, seed germination, tissue culture, proliferation

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