Study on the Seed Germination and Tissue Culture of Rosa graciliflora

doi:10.11924/j.issn.1000-6850.casb19030046

Abstract

Abstract:

This research aims to obtain a complete plant of Rosa graciliflora and establish its rapid propagation system. Germination rate of R. graciliflora seed was observed on different cold stratification time. Meanwhile, tender stem segments were used as explants to investigate the effects of different sterilized reagents, sterilized time and phytohormone combinations on the axillary bud induction and proliferation rate, and also rooting rate. Furthermore, the culture medium was optimized for each growth stage. The results showed that the seed germination rate was as high as 35% when treated by sand storage in low temperature for 9 months. The optimum combination for stem sterilization was 10% sodium hypochlorite solution for 15 min followed by 0.05% mercuric chloride solution for 10 min, and the optimal medium for bud proliferation was 1/2MS+NAA 0.2 mg/L+6-BA 2.0 mg/L. The rooting rate reached 90% in the rooting medium of WPM+IBA 0.3 mg/L. In conclusion, this research explores the seed germination condition of R. graciliflora and establishes its rapid propagation system, which could lay a foundation for the industrial production and further utilization of R. graciliflora.

Key words: Rosa graciliflora, seed germination, tissue culture, proliferation

CLC Number:

Q94-331

Li Huiling, Luo Yulan, Zhang Zhang, Yin Lijuan, Li Pujin, Zhang Dongmei. Study on the Seed Germination and Tissue Culture of Rosa graciliflora[J]. Chinese Agricultural Science Bulletin, 2020, 36(13): 89-93.

Figures/Tables 5

References 29

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序号	处理	播种时间	播种数量	发芽数量	发芽率%
1	对照	12月	100	0	0
2	低温沙藏3个月	3月	100	7.0	7.0
3	低温沙藏5个月	5月	100	19.0	19.0
4	低温沙藏7个月	7月	100	22.0	22.0
5	低温沙藏9个月	9月	100	35.0	35.0

序号	处理	播种时间	播种数量	发芽数量	发芽率%
1	对照	12月	100	0	0
2	低温沙藏3个月	3月	100	7.0	7.0
3	低温沙藏5个月	5月	100	19.0	19.0
4	低温沙藏7个月	7月	100	22.0	22.0
5	低温沙藏9个月	9月	100	35.0	35.0

序号	NAA浓度/(mg/L)	6-BA浓度/(mg/L)	KT浓度/(mg/L)	平均增殖系数	芽苗长势
1	0.2	1.0	0	0.5	丛生芽生长慢,叶色绿
2	0.2	1.5	0	1.4	丛生芽生长快,叶色绿
3	0.2	2.0	0	2.1	丛生芽生长快,叶色绿
4	0.2	3.0	0	0.2	丛生芽生长快,叶色黄绿
5	0	2.0	0.2	0	丛生芽生长慢,叶缘发黄
6	0	2.0	0.5	0	丛生芽生长慢,叶缘发黄

序号	NAA浓度/(mg/L)	6-BA浓度/(mg/L)	KT浓度/(mg/L)	平均增殖系数	芽苗长势
1	0.2	1.0	0	0.5	丛生芽生长慢,叶色绿
2	0.2	1.5	0	1.4	丛生芽生长快,叶色绿
3	0.2	2.0	0	2.1	丛生芽生长快,叶色绿
4	0.2	3.0	0	0.2	丛生芽生长快,叶色黄绿
5	0	2.0	0.2	0	丛生芽生长慢,叶缘发黄
6	0	2.0	0.5	0	丛生芽生长慢,叶缘发黄