Effects of Different Boron Deficiency Treatments on Leaf Growth and Photosynthetic Characteristics of Sugar Beet Seedlings

doi:10.11924/j.issn.1000-6850.casb19030017

Abstract

Abstract:

The effects of different boron deficiency treatments on the growth and photosynthesis of sugar beet leaves were discussed, aiming to provide a theoretical basis for production practice. In the present research, a hydroponic experiment was conducted to measure the leaf morphological parameters and photosynthetic performance of sugar beet seedlings under different boron concentrations of 0 μmol/L (B1), 0.5 μmol/L (B2), 5 μmol/L (B3) and 50 μmol/L (B4, CK) after 21 days. The results showed that boron deficiency significantly inhibited the growth and development of sugar beet leaves, and the inhibition degrees were related with the intensity of the stress. The specific performance was: boron deficiency significantly reduced leaf area, fresh and dry weight, increased leaf thickness and leaf color value; decreased leaf chlorophyll a and chlorophyll b by 22.3%-51.5% and 23.3%-42.3%, respectively; the photosynthetic parameters decreased by 0.6%-28.4% compared with the control in the treatment of boron deficiency. Correlation analysis showed that the correlation between leaf area and photosynthetic pigment, photosynthetic rate, transpiration rate and intercellular CO₂ concentration under boron deficiency reached very significant level (P<0.01). In conclusion, boron deficiency affects the leaf morphology of plants, inhibits the formation of photosynthetic pigments, significantly reduces the photosynthetic characteristics of leaves, and further reduces the biomass accumulation of sugar beet seedlings, leading to abnormal growth and development of leaves.

Key words: boron, sugar beet, growth parameters, photosynthetic characteristics

CLC Number:

S566.3

Hao Xueming, Wang Xiangling, Wu Zhenzhen, Song Baiquan, Zhou Jianchao. Effects of Different Boron Deficiency Treatments on Leaf Growth and Photosynthetic Characteristics of Sugar Beet Seedlings[J]. Chinese Agricultural Science Bulletin, 2020, 36(18): 27-32.

Figures/Tables 5

References 26

[1]	Warington K. The effect of boric acid and borax on the broad bean and certain other plants[J]. Ann Bot, 1923,37:629-672.
[2]	黄宗安, 苏世闻, 史建磊, 等. 不同供硼水平对芜菁幼苗叶片光合气体交换、叶绿素荧光和植株生长的影响[J]. 浙江农业学报, 2015,27(8):1403-1407.
[3]	祁寒, 孙光明, 李绍鹏, 等. 硼在作物生长过程中的研究现状[J]. 安徽农业科学, 2008,36(16):6649-6650,6652.
[4]	屈红征, 王丽萍, 吴国良. 植物硼素营养研究进展[J]. 山西农业大学学报, 2001(02):173-176.
[5]	Cooke D A, Scott R K. The Sugar Beet Crop[M]. Springer Netherlands, 1993.
[6]	孟凡华, 刘才, 李仲奎. 硼对甜菜生长发育及产量影响[J]. 土壤肥料, 1995(03):47.
[7]	石磊, 徐芳森. 植物硼营养研究的重要进展与展望[J]. 植物学通报, 2007,24(6):789-798.
[8]	卢晓佩, 姜存仓, 董肖昌, 等. 硼胁迫下不同柑橘砧木叶片物质组成及结构的FTIR表征[J]. 光谱学与光谱分析, 2017,37(05):1380-1385.
[9]	陈晟, 施木田, 吴宇芬, 等. 硼水平对不同类型西瓜生长的影响[J]. 中国果树, 2016(02):43-47.
[10]	周金忠, 潘学军, 黄玫, 等. 不同供硼水平对野生毛葡萄试管苗生长的影响[J]. 广东农业科学, 2013,40(04):26-29.
[11]	El-Shintinawy F. Structural and functional damage caused by boron deficiency in sunflower leaves[J]. Photosynthetica, 1999,36(4):565-573. doi: 10.1023/A:1007096105491 URL
[12]	焦晓燕, 王劲松, 武爱莲, 等. 缺硼对绿豆叶片光合特性和碳水化合物含量的影响[J]. 植物营养与肥料学报, 2013,19(03):615-622.
[13]	Dell B, Huang L B. Physiological response of plants to low boron[J]. Plant Soil, 1997,193(1):103-120. doi: 10.1023/A:1004264009230 URL
[14]	杨兵, 张红. 缺硼对黄瓜幼苗生长和光合特性的影响[J]. 园艺与种苗, 2011(05):88-90.
[15]	柴喜荣, 于文杰, 杨暹, 等. 不同供硼水平对菜心光合作用和品质的影响[J]. 广东农业科学, 2013,40(12):37-39.
[16]	从心黎. 缺硼对樱桃萝卜形态结构和生理机制影响的研究[D]. 海南大学, 2014.
[17]	Li M, Zhao Z, Zhang Z, et al. Effect of boron deficiency on anatomical structure and chemical composition of petioles and photosynjournal of leaves in cotton (Gossypium hirsutum L.)[J]. Scientific Reports, 2017,7(1):4420. doi: 10.1038/s41598-017-04655-z URL
[18]	Sim N I, D Az L Pez L, Gimeno V, et al. Effects of boron excess in nutrient solution on growth,mineral nutrition,and physiological parameters of Jatropha curcas seedlings[J]. Plant NutrSoil Sci, 2013,176(2):165-174.
[19]	Wu X W, Lu X P, Muhammad R, et al. Boron deficiency and toxicity altered the subcellular structure and cell wall composition architecture in two citrus rootstocks[J]. Scientia Horticulturae, 2018,238:147-154. doi: 10.1016/j.scienta.2018.04.057 URL
[20]	Chen M, Sasmita M, Scott A H, et al. Proteomic analysis of Arabidopsis thaliana leaves in response to acute boron deficiency and toxicity reveals effects on photosynjournal, carbohydrate metabolism, and protein synjournal[J]. Journal of Plant Physiology, 2014,171(3-4):235-242. doi: 10.1016/j.jplph.2013.07.008 URL
[21]	肖万欣, 刘晶, 史磊, 等. 氮密互作对不同株型玉米形态、光合性能及产量的影响[J]. 中国农业科学, 2017,50(19):3690-3701. doi: 10.3864/j.issn.0578-1752.2017.19.006 URL
[22]	姚宇洁, 姜存仓. 缺铁胁迫柑橘砧木幼苗的光合特性和叶绿体超微结构[J]. 植物营养与肥料学报, 2017,23(5):1345-1351.
[23]	冯鹏, 孙力, 申晓慧, 等. 不同诱变处理对苜蓿叶绿素含量及光合作用的影响[J]. 中国农学通报, 2018,34(23):122-128.
[24]	Han S, Chen L S, Jiang H X, et al. Boron deficiency decreases growth and photosynjournal, and increases starch and hexoses in leaves of citrus seedlings[J]. Journal of Plant Physiology, 2008,165(13):1331-1341. doi: 10.1016/j.jplph.2007.11.002 URL pmid: 18191499
[25]	Chen M, Mishra S, Heckathorn S A, et al. Proteomic analysis of Arabidopsis thaliana leaves in response to acute boron deficiency and toxicity reveals effects on photosynjournal, carbohydrate metabolism, and protein synjournal[J]. Journal of Plant Physiology, 2014,171(3-4):235-242. doi: 10.1016/j.jplph.2013.07.008 URL
[26]	Soheil K, Vahid T, Michelle W. Boron amendment improves water relations and performance of Pistacia vera under salt stress[J]. Scientia Horticulturae, 2018,241:252-259. doi: 10.1016/j.scienta.2018.06.064 URL

相关系数	叶面积	Chla	Chlb	Chla/b	Car	Pn	Tr	Ci	Gs
叶面积	1
Chla	0.877^**	1
Chlb	0.814^**	0.971^**	1
Chla/b	0.758^**	0.658^*	0.465	1
Car	0.879^**	0.999^**	0.971^**	0.658^*	1
Pn	0.751^**	0.717^**	0.603^*	0.775^**	0.722^**	1
Tr	0.828^**	0.842^**	0.809^**	0.588^*	0.850^**	0.884^**	1
Ci	0.836^**	0.789^**	0.710^**	0.721^**	0.789^**	0.915^**	0.854^**	1
Gs	0.549	0.606^*	0.644^*	0.222	0.618^*	0.578^*	0.826^**	0.431	1

相关系数	叶面积	Chla	Chlb	Chla/b	Car	Pn	Tr	Ci	Gs
叶面积	1
Chla	0.877^**	1
Chlb	0.814^**	0.971^**	1
Chla/b	0.758^**	0.658^*	0.465	1
Car	0.879^**	0.999^**	0.971^**	0.658^*	1
Pn	0.751^**	0.717^**	0.603^*	0.775^**	0.722^**	1
Tr	0.828^**	0.842^**	0.809^**	0.588^*	0.850^**	0.884^**	1
Ci	0.836^**	0.789^**	0.710^**	0.721^**	0.789^**	0.915^**	0.854^**	1
Gs	0.549	0.606^*	0.644^*	0.222	0.618^*	0.578^*	0.826^**	0.431	1