Corncob Hemicellulose Hydrolysate: Optimized Pretreatment and Detoxification Process

doi:10.11924/j.issn.1000-6850.casb20190400042

Abstract

Abstract:

To reduce the content of toxic substances such as acetic acid, furfural and phenols in the corncob hemicellulose hydrolyzate, and to increase the available sugar content, corncob was used as raw material to prepare hydrolysate, and the pretreatment and detoxification process were optimized, and the optimization effect was detected by high performance liquid chromatography (HPLC). The results showed that the removal rate of acetic acid and phenolic substances could be increased to 93.4% and 32.3%, respectively, after the 10~20 mesh corncob was soaked in 10% ammonia water for 24 h, and the content of furfural was also greatly reduced. After vacuum concentration, CaO detoxification and 2% activated carbon adsorption, the content of toxic substances was reduced again. At this time, the hydrolyzate could be completely used for the strain fermentation, and the purpose of removal was achieved. The optimal hydrolyzate treatment step obtained in this study could meet the standards for the fermented strain, indicating that the treatment method of hydrolyzate directly affects the preparation of hydrolyzate. The study provides an idea for the research and utilization of renewable resources such as lignocellulose, and lays a research foundation for fermentation production.

Key words: corncob hemicellulose hydrolysate, pretreatment, detoxification, optimization

CLC Number:

S216.2

Wang Jiawang, Yang Xiaofeng, Liu Guoming, Ge Jingping. Corncob Hemicellulose Hydrolysate: Optimized Pretreatment and Detoxification Process[J]. Chinese Agricultural Science Bulletin, 2020, 36(20): 28-35.

Figures/Tables 9

References 35

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氨水浓度	物质种类
氨水浓度	葡萄糖	木糖	阿拉伯糖	乙酸	糠醛
0%氨水	0.24	6.22	0.36	0.41	0.037
2%氨水	0.17	1.91	0.10	0.035	0.011
5%氨水	0.20	1.53	0.49	0.033	0.002
8%氨水	0.52	3.63	0.42	0.035	0
10%氨水	0.13	5.77	0.30	0.006	0
15%氨水	0.09	3.55	0.06	0.0057	0
18%氨水	0.10	2.74	0.07	0	0

氨水浓度	物质种类
氨水浓度	葡萄糖	木糖	阿拉伯糖	乙酸	糠醛
0%氨水	0.24	6.22	0.36	0.41	0.037
2%氨水	0.17	1.91	0.10	0.035	0.011
5%氨水	0.20	1.53	0.49	0.033	0.002
8%氨水	0.52	3.63	0.42	0.035	0
10%氨水	0.13	5.77	0.30	0.006	0
15%氨水	0.09	3.55	0.06	0.0057	0
18%氨水	0.10	2.74	0.07	0	0