The Function of Defense Signaling Pathways: Tobacco Resistance to Myzus persicae Induced by Bemisia tabaci

doi:10.11924/j.issn.1000-6850.casb20191201004

Abstract

Abstract:

Bemisia tabaci infection can induce the tobacco resistance to Myzus persicae. The study aims to investigate the relationship between the defense signaling of salicylic acid (SA) and jasmonic acid (JA) and the resistance to Myzus persicae induced by Bemisia tabaci. The effects of Bemisia tabaci nymphs feeding on SA and JA level and downstream defense genes expressing level were tested, and the effects of exogenous SA and JA on the growth and development of Myzus persicae were analyzed by biochemical analysis and real-time fluorescent quantitative PCR. The results showed that Myzus persicae infection obviously activated SA defense pathway. After feeding 15 d, the SA level was 1.40-fold higher than the control, and the levels of PR-1a and PR-2a were increased by 3.22 times and 0.74 times, respectively. JA level was not obviously affected, while the transcript levels of JA downstream defense genes PI-II and TPI were decreased by 73.91% and 56.73%, respectively. Biochemical analysis revealed that the application of exogenous SA was harmful to the survival and the relative average growth rate of M. persicae. Treating with 1 mmol/L SA, aphid survival and growth rate were decreased by 43.2% and 11.54%, respectively, compared with the control. Methyl jasmonate had no harmful effect on the growth and development of Myzus persicae. In conclusion, SA mediated defense response contributes to the tobacco resistance against M. persicae.

Key words: Bemisia tabaci, interspecific competition, jasmonic acid, Myzus persicae, salicylic acid, induced defense

CLC Number:

S436.429

Xu Yanan, Sun Xia, Zhao Haipeng, Xue Ming. The Function of Defense Signaling Pathways: Tobacco Resistance to Myzus persicae Induced by Bemisia tabaci[J]. Chinese Agricultural Science Bulletin, 2020, 36(36): 93-99.

Figures/Tables 5

References 31

[1]	Li Q, Xie Q G, Smith-Becker J, et al. Mi-1-Mediated aphid resistance involves salicylic acid and mitogen-activated protein kinase signaling cascades[J]. Molecular plant-microbe interactions: MPMI, 2006,19(6):655-664. doi: 10.1094/MPMI-19-0655 URL pmid: 16776299
[2]	Maleck K, Levine A, Eulgem T. The transcriptome of Arabidopsis thaliana during systemic acquired resistance[J]. Nature genetics, 2000,26(4):403-410. URL pmid: 11101835
[3]	Reymond P, Weber H, Damond M. Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis[J]. The Plant cell, 2000,12(5):707-720. URL pmid: 10810145
[4]	Thompson Gary A, Goggin Fiona L. Transcriptomics and functional genomics of plant defence induction by phloem-feeding insects[J]. Journal of experimental botany, 2006,57(4):755-766. doi: 10.1093/jxb/erj135 URL pmid: 16495409
[5]	Truitt C L, Wei H X, Pare P W. A plasma membrane protein from Zea mays binds with the herbivore elicitor volicitin[J]. Plant Cell, 2004,16(2):523-532. URL pmid: 14729912
[6]	Voelckel C, Weisser W W, Baldwin I T. An analysis of plant-aphid interactions by different microarray hybridization strategies[J]. Molecular Ecology, 2004,13:3187-3195. doi: 10.1111/j.1365-294X.2004.02297.x URL pmid: 15367131
[7]	黎家, 李传友. 新中国成立70年来植物激素研究进展[J]. 中国科学:生命科学, 2019,49(10):1227-1281.
[8]	俞晓平, 何瑜晨, 郦卫弟, 等. 水杨酸信号路径在调控入侵生物烟粉虱诱导植物间接防御中的作用[J]. 中国计量大学学报, 2017,28(1):1-6,34.
[9]	吴德伟, 汪姣姣, 谢道昕. 茉莉素与植物生物胁迫反应[J]. Biotechnology Bulletin, 2018(7):14-23.
[10]	巩莎. MeJA对枣树抗虫性的诱导及其对日本龟蜡蚧的影响[D]. 太原:山西大学, 2012.
[11]	Cao H H, Wang S H, Liu T X. Jasmonate- and salicylate-induced defenses in wheat affect host preference and probing behavior but not performance of the grain aphid, Sitobion avenae[J]. Insect science, 2013. doi: 10.1111/1744-7917.12873 URL pmid: 32965085
[12]	戈峰, 吴孔明, 陈学新. 植物-害虫-天敌互作机制研究前沿[J]. 应用昆虫学报, 2011,48(1):1-6.
[13]	Zarate S I, Kempema L A, Walling L L. Silver leaf whitefly induces salicylic acid defenses and suppresses effectual jasmonic acid defenses[J]. Plant physiology, 2007,143(2):866-875. doi: 10.1104/pp.106.090035 URL pmid: 17189328
[14]	Sarmento R A, Lemos F, Bleeker P M, et al. A herbivore that manipulates plant defence[M]// Ecology Letters. 2011.
[15]	Pascual S, Callejas C. Intra- and interspecific competition between biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) from Spain[J]. Bulletin of entomological research, 2004,94(4):369-375. URL pmid: 15301702
[16]	罗晨, 姚远, 王戎疆, 等. 利用mtDNA CO I基因序列鉴定我国烟粉虱的生物型[J] . 昆虫学报, 2002,45(6):759-763.
[17]	王洪涛, 薛明, 李庆亮, 等. B型烟粉虱取食诱导的烟草对斜纹夜蛾生长发育和繁殖的影响及机制探讨[J]. 中国农业科学, 2011,44(22):4600-4609. doi: 10.3864/j.issn.0578-1752.2011.22.006 URL
[18]	李庆亮. B型烟粉虱诱导烟草对烟蚜的防御反应及机制探讨[C]. 中国植物保护学会(China Society of Plant Protection).公共植保与绿色防控.中国植物保护学会, 2010: 756.
[19]	Zhang P J, Li W D, Huang F, et al. Feeding by Whiteflies Suppresses Downstream Jasmonic Acid Signaling by Eliciting Salicylic Acid Signaling[J]. Journal of chemical ecology, 2013,39(5):612-619. URL pmid: 23604702
[20]	李庆亮, 谭伟, 薛明. B型烟粉虱危害对烟草叶片光系统Ⅱ的影响[J]. 中国农业科学, 2012,45(19):3988-3995. doi: 10.3864/j.issn.0578-1752.2012.19.010 URL
[21]	Liu S S, De Barro P J, Xu J, et al. Asymmetric Mating Interactions Drive Widespread Invasion and Displacement in a Whitefly[J]. Science, 2007,318(5857):1769-1772. doi: 10.1126/science.1149887 URL pmid: 17991828
[22]	Haipeng Z, Xiaoying Z, Ming X, et al. Feeding of Whitefly on Tobacco Decreases Aphid Performance via Increased Salicylate Signaling[J]. PLOS ONE, 2015,10(9):e0138584. doi: 10.1371/journal.pone.0138584 URL pmid: 26381273
[23]	Liang P, Cui J Z, Yang X Q. Effects of host plants on insecticide susceptibility and carboxylesterase activity in Bemisia tabaci biotype B and greenhouse whitefly, Trialeurodes vaporariorum[J]. Pest management science, 2007,63(4):365-371. doi: 10.1002/ps.1346 URL pmid: 17323411
[24]	Luan J B, Li J M, Varela N. Global analysis of the transcriptional response of whitefly to tomato yellow leaf curl China virus reveals the relationship of coevolved adaptations[J]. Journal of virology, 2011,85(7):3330-3340. doi: 10.1128/JVI.02507-10 URL pmid: 21270146
[25]	王承香, 薛明, 毕明娟, 等. B型烟粉虱取食诱导烟草对烟蚜防御反应的时间效应[J]. 昆虫学报, 2010,53(3):314-322.
[26]	王霞. 不同取食习性害虫和beta一葡萄糖昔酶对水稻体内重要防御相关信号分子含量的影响[D]. 杭州:浙江大学, 2006.
[27]	Xue M, Wang C X, Bi M J, et al. Induced defense by Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) in tobacco against Myzus persicae (Hemiptera: Aphididae)[J]. Environmental Entomology, 2010,39(3):883-891. URL pmid: 20550802
[28]	Kempema Louisa A, Cui X P, Holzer Frances M, et al. Arabidopsis transcriptome changes in response to phloem-feeding silverleaf whitefly nymphs. Similarities and distinctions in responses to aphids[J]. Plant physiology, 2007,143(2):849-865. URL pmid: 17189325
[29]	Mohase L, Westhuizen A J V D. Salicylic acid is involved in resistance responses in the Russian wheat aphid-wheat interaction[J]. Journal of Plant Physiology, 2002,159(6):585-590. doi: 10.1078/0176-1617-0633 URL
[30]	María Gloria Estrada-Hernández, José Humberto Valenzuela-Soto, Enrique Ibarra-Laclette, et al. Differential gene expression in whitefly Bemisia tabaci-infested tomato (Solanum lycopersicum) plants at progressing developmental stages of the insect's life cycle[J]. Physiologia Plantarum, 2009,137(1). doi: 10.1111/j.1399-3054.2009.01247.x URL pmid: 19493310
[31]	娄永根, 程家安. 植物的诱导抗虫性[J]. 昆虫学报, 1997,40(3):320-331.

基因	登录号	引物
PR-1a	X05959.1	F: 5’-GATTGTAACCTCGTACATTCTCATG-3’ R: 5’-GCCAGCCACTTTCAGATACAATA-3’
PR-2a	M59443.1	F: 5’-TAACCTTCCACTCTTAGCCAATG-3’ R: 5’-GCCAGCCACTTTCAGATACAATA-3’
PI-II	Z29537.1	F: 5’-TAGTTTCGTCGCTCATCT-3’ R: 5’-TAGGGTCACATTCCGTAG-3’
TPI	DQ158200.1	F: 5'-TCAGGAGATAGTAAATATGGCTGTTCA-3’ R: 5'-ATCTGCATGTTCCACATTGCTTA-3’
Actin	X69885.1	F:5’-GCTTGCTTACATTGCTCTCGACTAT-3’ R:5’-GATAGAGTTGTATGTAGTCTCGTG-3’

基因	登录号	引物
PR-1a	X05959.1	F: 5’-GATTGTAACCTCGTACATTCTCATG-3’ R: 5’-GCCAGCCACTTTCAGATACAATA-3’
PR-2a	M59443.1	F: 5’-TAACCTTCCACTCTTAGCCAATG-3’ R: 5’-GCCAGCCACTTTCAGATACAATA-3’
PI-II	Z29537.1	F: 5’-TAGTTTCGTCGCTCATCT-3’ R: 5’-TAGGGTCACATTCCGTAG-3’
TPI	DQ158200.1	F: 5'-TCAGGAGATAGTAAATATGGCTGTTCA-3’ R: 5'-ATCTGCATGTTCCACATTGCTTA-3’
Actin	X69885.1	F:5’-GCTTGCTTACATTGCTCTCGACTAT-3’ R:5’-GATAGAGTTGTATGTAGTCTCGTG-3’