Effects Induced by Alicylic Acid on Defense Enzyme Activities in Apple

doi:10.11924/j.issn.1000-6850.casb19030018

Abstract

Abstract:

To study the activities of defense enzymes in apple by leaf-spraying salicylic acid (SA), the resistant variety ‘Kitanosach’ and the susceptible variety ‘Liquanduanfu’ were used as materials, the activities of defense enzymes in the leaves were detected before and after different-concentration SA treatments. The results showed that after treated by salicylic acid, the β-1,3-glucosidase, POD, PAL and PPO activities of ‘Kitanosach’ and ‘Liquanduanfu’ increased and were higher than those of the control. The activity of β-1,3-glucanase, POD, PAL and PPO in ‘Kitanosach’ was 2.04 times, 6.19 times, 2.73 times and 2.32 times of those of the control, respectively. The activity of β-1,3-glucanase, PAL POD and PPO in ‘Liquanduanfu’ was 1.82 times, 1.70 times, 5.68 times and 4.00 times of those of the control, respectively. Therefore, the activities of defense enzymes can be increased by different degrees after treated by salicylic acid, but the resistance of different varieties was different.

Key words: apple, salicylic acid, enzyme activity, induced resistance

CLC Number:

S432.1

Hou Hui, Zhou Zengqiang, Wang Li, Wang Shengrong. Effects Induced by Alicylic Acid on Defense Enzyme Activities in Apple[J]. Chinese Agricultural Science Bulletin, 2020, 36(18): 39-43.

Figures/Tables 4

References 28

[1]	Fenner E A. A rot of apples caused by Botryosphaeria ribis[J]. Phytopathology, 1925,15:130-134.
[2]	Smith C O. Inoculation showing the wide host range of Botryosphaeria ribis[J]. Agri Res, 1934,49:467-476.
[3]	孙柏欣, 刘长远, 陈彦, 等. 5种杀菌剂防治苹果轮纹病试验[J]. 北方果树, 2008(3):16-17.
[4]	高艳敏, 沈永波, 张恩尧, 等. 苹果轮纹病发生规律及条件的研究[J]. 安徽农业科学, 2007,3:751-754.
[5]	康玲, 郝红梅, 杨振英, 等. 苹果轮纹病研究进展[J]. 中国农学通报, 2009,25(09):188-191.
[6]	国立耘, 李金云, 李保华, 等. 中国苹果枝干轮纹病发生和防治情况[J]. 植物保护, 2009,35(4):120-123.
[7]	郭泺, 刘振宇. 论苹果病害IPM 系统中的化学防治和生物防治[J]. 山东林业科技, 2000,1:44-47.
[8]	李保华, 王彩霞, 董向丽. 中国苹果主要病害研究进展与病害防治中的问题[J]. 植物保护, 2013,39(5):46-54.
[9]	Hammerschmidt R. Induced disease resistance: how do induced plants stop pathogens[J]. Physiological and Molecular Plant Pathology, 1999(55):77-84.
[10]	Ryals J A, Neuenschwander U H, Willits M G, et al. Systemic acquired resistance[J]. Plant Cell, 1996(8):1809-1819.
[11]	Schenk P M, Kazan K, Wilson I, et al. Coordinated plant defense responses in Arabidopsis revealed by microarray analysis[J]. Proc. Natl. Acad. Sci. USA, 2000(97):11655-11660.
[12]	Bent A F. Plant disease resistance genes: Function meets structure[J]. Plant Cell, 1996,8(10):1757-1771. doi: 10.1105/tpc.8.10.1757 URL pmid: 12239361
[13]	尹玲莉, 侯晓杰. 植物抗性信号分子-水杨酸研究进展[J]. 中国农学通报, 2007,23(1):338-342.
[14]	Blanco F, Salinas P, Cecchini N M, et al. Early genomic responses to salicylic acid in Arabidopsis[J]. Plant Mol Biol, 2009,70(1-2):79-102. doi: 10.1007/s11103-009-9458-1 URL
[15]	Malamy J, Carr J P, Klessig D F, et al. Salicylic acid: A likely endogenous signal in the resistance response of tobacco to viral infection[J]. 科学(上海), 1990(4983):1002-1004.
[16]	Malamy J, Henning J, Klessing D F. Temperature-Depended induction of salicylic acid and its conjugates during the resistance response to tobacco mosaic virus infection[J]. Plant Cell, 1992(3):359-366. doi: 10.1105/tpc.4.3.359 URL pmid: 12297650
[17]	Rasmussen J B, Hammerschmidt R, Zook M N. Systemic induction of salicylic acid accumulation in cucumber after inoculation with Pseudomonas syringae PV. syringae[J]. Plant Physiol, 1991,97:1342-1347. doi: 10.1104/pp.97.4.1342 URL pmid: 16668554
[18]	Ghaouth A E I, Wilson C L, Callahan A M. Induction of chitinase, β-1,3-glucanase, andphenylalanine ammonia lyase in peach fruit by UV-C treatment[J]. Phytopathology J, 2003,93(3):349-355.
[19]	Kuc J. Cellular and Molecular Biology of Plant Strees[M]. Alan R Liss Inc, 1985: 303-318.
[20]	White R F. Acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco[J]. Virology, 1979(2):410-412. doi: 10.1016/0042-6822(73)90182-7 URL pmid: 4355113
[21]	Van Loon L C. The induction of pathogenesis-related protein by pathogens and specific chemicals[J]. Neth J Plant Pathol, 1983,89:265-273. doi: 10.1007/BF01995261 URL
[22]	杜良成, 王钧. 病原相关蛋白及其在植物抗病中的作用[J]. 植物生理学通讯, 1990(4):1-6.
[23]	Van Loon L C. Induced resistance in plants and the role of pathogenesis-related proteins[J]. European Journal of Plant Pathology, 1997(9):753-765.
[24]	Dong H Z, Cohen Y. Extracts of killed Penicillium chrysogenum induce resistance against Fusarium wilt of melon[J]. Phytoparasitica, 2001,29.
[25]	王廷璞, 马静静, 赵菲佚. β-1,3-葡聚糖酶和几丁质酶在农作物病虫害防治中的研究进展[J]. 安徽农业科学, 2010,38(26):14417-14419.
[26]	陈水源, 陈昆松, 刘卫红. 植物苯丙氨酸解氨酶基因的表达调控与研究展望[J]. 果树学报, 2003,20(5):351-357.
[27]	魏国强, 朱祝军, 钱群秋. 硅对黄瓜白粉病抗性的影响及其生理机制[J]. 植物营养与肥料学报, 2004,10(2):202-205. doi: 10.11674/zwyf.2004.0218 URL
[28]	郭红莲, 程根武, 陈捷. 玉米灰斑病抗性反应中酚类物质代谢作用的研究[J]. 植物病理学报, 2003,33(4):343-346.