Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (27): 1-5.doi: 10.11924/j.issn.1000-6850.casb2020-0863

Special Issue: 水稻

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Genetic Transformation of Rice: Research Progress

Peng Xiaoqun(), Wang Menglong()   

  1. School of Life Sciences, Huizhou University, Huizhou Guangdong 516007
  • Received:2021-01-04 Revised:2021-04-13 Online:2021-09-25 Published:2021-10-28
  • Contact: Wang Menglong E-mail:1290882614@qq.com;wml0824@126.com

Abstract:

Rice genetic transformation technology is an important mean to explore the function of rice genes and carry out genetic breeding. In order to promote the application of rice genetic transformation, we summarized several genetic transformation methods, such as polyethylene glycol (PEG) mediated method, liposome method, electric shock method, particle bombardment, pollen tube pathway method and Agrobacterium-mediated method. Furthermore, the advantages and disadvantages of these genetic transformation methods in practical application were described. Meanwhile, we focused on the research progress of Agrobacterium-mediated transformation of rice as well as pointed out the influence of infection system, regeneration system and tissue culture conditions on Agrobacterium-mediated transformation of rice. Moreover, some specific measures for optimization of Agrobacterium-mediated transformation were also provided.

Key words: rice, genetic transformation, Agrobacterium-mediated transformation, influencing factors, transgene

CLC Number: