Subcellular Distribution and Chemical Morphological Characteristics of Lead in Plants: Improvement of Determination Method

doi:10.11924/j.issn.1000-6850.casb20200100048

Abstract

Abstract:

To improve the determination accuracy of lead subcellular distribution and chemical morphological characteristics in plant, the torpedograss was used as material, and different extraction methods were applied to test the distribution characteristics of lead in torpedograss roots. The proportion relationship between the quality of torpedograss material and the volume of extracted liquid was analyzed with quantitative study. And then the heavy metal extraction process was improved. The results showed that when the ratio between the volume of plant ground material and the total volume of the extract was 1:5, the extraction effect of each chemical binding state was better. The recovery rate of lead reached 97.69%. After determining the F2 component of subcellular distribution of lead in torpedograss roots, the extraction effect was the best when the temperature of the heating plate was set at 250℃ for 0.5 h and then at 145℃ for 2 h. The lead content in residue state was about 0 μg/g and the overall recovery rate was up to 98.38%. Based on the modified method, the measurement precision and measurement time were improved. The research results provide reference for the accurate determination of the subcellular distribution and chemical morphological characteristics of other metal in plant.

Key words: torpedograss, lead, subcellular distribution, chemical morphological characteristics, electrothermal digestion

CLC Number:

Lin Jing, Li Feng, Liu Yuntao, Lin Xiaoxuan, Gao Guijuan. Subcellular Distribution and Chemical Morphological Characteristics of Lead in Plants: Improvement of Determination Method[J]. Chinese Agricultural Science Bulletin, 2020, 36(36): 74-78.

Figures/Tables 3

References 22

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材料鲜重/g	乙醇	去离子水	氯化钠	醋酸	盐酸	根部总铅回收率/%
0.1	146.28±29.36	523.16±123.36	1264.42±320.36	1726.94±191.6	537.82±179.36	85.25
0.2	191.85±53.53	603.06±110.18	1351.36±216.77	2081.60±261.7	583.23±87.54	97.69
0.4	168.35±37.27	617.35±78.638	1302.72±208.65	2110.46±188.7	517.82±56.17	95.77

材料鲜重/g	乙醇	去离子水	氯化钠	醋酸	盐酸	根部总铅回收率/%
0.1	146.28±29.36	523.16±123.36	1264.42±320.36	1726.94±191.6	537.82±179.36	85.25
0.2	191.85±53.53	603.06±110.18	1351.36±216.77	2081.60±261.7	583.23±87.54	97.69
0.4	168.35±37.27	617.35±78.638	1302.72±208.65	2110.46±188.7	517.82±56.17	95.77

处理浓度	处理天数/d	消煮时间/h	铅提取量/(μg/g鲜重)
400 μg/g	5	1.5	43.31±5.19B
		2.5	61.94±5.00A
		3.5	60.51±5.89A
400 μg/g	25	1.5	50.79±7.95B
		2.5	69.94±4.00A
		3.5	70.50±6.33A
800 μg/g	5	1.5	65.04±7.86 B
		2.5	75.41±19.59A
		3.5	73.97±6.68 A
800 μg/g	25	1.5	87.64±9.16 B
		2.5	122.96±9.05 A
		3.5	126.76±11.09A

处理浓度	处理天数/d	消煮时间/h	铅提取量/(μg/g鲜重)
400 μg/g	5	1.5	43.31±5.19B
		2.5	61.94±5.00A
		3.5	60.51±5.89A
400 μg/g	25	1.5	50.79±7.95B
		2.5	69.94±4.00A
		3.5	70.50±6.33A
800 μg/g	5	1.5	65.04±7.86 B
		2.5	75.41±19.59A
		3.5	73.97±6.68 A
800 μg/g	25	1.5	87.64±9.16 B
		2.5	122.96±9.05 A
		3.5	126.76±11.09A

处理浓度/(μg/g)	处理天数/d	F1组分铅含量/(μg/g鲜重)	F2组分铅含量/(μg/g鲜重)	根部总提取量/(μg/g鲜重)
400	5	3045.58±163.00	1795.12±99.00	5078.79±125.26
400	25	3321.20±207.00	1356.02±89.20	4924.69±664.00
800	5	2659.04±141.00	1104.41±93.45	4061.64±619.59
800	25	3638.72±136.00	1526.80±112.31	5374.75±560.09