Effects of Exogenous H2O2 on Physiological Characteristics of Populus tomentosa Under Saline-alkali Stress

doi:10.11924/j.issn.1000-6850.casb20200200093

Abstract

Abstract:

By studying the influence of exogenous H₂O₂ on physiological characteristics of Populus tomentosa under saline-alkali stress, the paper aims to provide a theoretical basis to improve the afforestation of P. tomentosa in saline-alkali land. Under potted condition, four treatments were set including nutrient solution (control, H1), nutrient solution+NaHCO₃+NaCl (H2), nutrient solution+NaHCO₃+NaCl+H₂O₂(H3), and nutrient solution+H₂O₂(H4), which were repeated for 3 times. The results showed that: the content of superoxide anion, hydrogen peroxide, MDA and conductivity of P. tomentosa of H2 was significantly higher than those of H1, and the content of superoxide anion and hydrogen peroxide of H3 was significantly lower than those of H2, while the decrease of conductivity and MDA content was not significant, and there was no significant difference between H4 and H1; the activity of SOD, POD, CAT and APX of H2 was significantly higher than those of H1, increasing the content of AsA and GSH, H3 had an increase in the activity of protective enzyme and the activity of H₂O₂ scavenging system of P. tomentosa compared with H2, while H4 significantly increased the AsA content of P. tomentosa compared with H1; H2 significantly increased the content of soluble sugar of P. tomentosa compared with H1, while H3 decreased the soluble sugar content compared with H2; the content of soluble protein and free proline of P. tomentosa were increased by H1 compared with H2 and by H3 compared with H2. Through the comprehensive analysis, it is believed that exogenous H₂O₂ can stimulate the physiological response mechanism of P. tomentosa and alleviate salinity stress significantly.

Key words: saline-alkali stress, H₂O₂, Populus tomentosa, physiological response, protective enzyme, MDA

CLC Number:

S723

Ren Jinlan. Effects of Exogenous H₂O₂ on Physiological Characteristics of Populus tomentosa Under Saline-alkali Stress[J]. Chinese Agricultural Science Bulletin, 2021, 37(7): 43-48.

Figures/Tables 5

References 29

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处理	7月4日		7月16日
处理	超氧阴离子/(μg/g)	过氧化氢/(mg/g)	超氧阴离子/(μg/g)	过氧化氢/(mg/g)
H1(CK)	0.22±0.03c	0.85±0.04a	0.29±0.06c	0.93±0.05c
H2	2.05±0.11a	2.13±0.21b	2.35±0.10a	2.38±0.14a
H3	1.52±0.12b	1.41±0.10c	1.67±0.12b	1.69±0.03b
H4	0.16±0.02c	0.73±0.16c	0.21±0.05c	0.77±0.04c

处理	7月4日		7月16日
处理	超氧阴离子/(μg/g)	过氧化氢/(mg/g)	超氧阴离子/(μg/g)	过氧化氢/(mg/g)
H1(CK)	0.22±0.03c	0.85±0.04a	0.29±0.06c	0.93±0.05c
H2	2.05±0.11a	2.13±0.21b	2.35±0.10a	2.38±0.14a
H3	1.52±0.12b	1.41±0.10c	1.67±0.12b	1.69±0.03b
H4	0.16±0.02c	0.73±0.16c	0.21±0.05c	0.77±0.04c

处理	7月4日		7月16日
处理	相对电导率/%	MDA/(nmol/g)	相对电导率/%	MDA/(nmol/g)
H1(CK)	17.48±1.20b	4.54±0.50b	24.57±2.51b	6.25±0.40b
H2	27.59±2.20a	12.44±1.12a	37.56±2.31a	15.98±1.90a
H3	25.52±2.12a	11.24±1.28a	35.85±2.25a	14.22±2.01a
H4	16.35±2.02b	5.56±0.51b	22.72±1.65b	7.37±0.72b

处理	7月4日		7月16日
处理	相对电导率/%	MDA/(nmol/g)	相对电导率/%	MDA/(nmol/g)
H1(CK)	17.48±1.20b	4.54±0.50b	24.57±2.51b	6.25±0.40b
H2	27.59±2.20a	12.44±1.12a	37.56±2.31a	15.98±1.90a
H3	25.52±2.12a	11.24±1.28a	35.85±2.25a	14.22±2.01a
H4	16.35±2.02b	5.56±0.51b	22.72±1.65b	7.37±0.72b

处理	7月4日				7月16日
处理	SOD	POD	CAT	APX	SOD	POD	CAT	APX
H1(CK)	15.57±1.50c	10.68±1.00c	7.25±0.80c	2.36±1.31c	23.32±1.31c	12.01±1.29c	10.53±0.76c	3.58±0.50c
H2	21.46±1.00b	12.35±1.28b	8.35±0.59b	4.66±1.99b	32.45±1.89b	18.87±0.80b	12.35±0.59b	5.79±0.70b
H3	25.97±2.00a	15.45±1.23a	10.72±0.55a	7.86±2.60a	37.97±2.60a	20.15±0.99a	13.55±0.50a	8.97±0.90a
H4	18.68±1.30bc	9.68±0.61c	7.53±0.98c	3.13±1.60c	27.69±1.57c	13.65±1.31c	10.68±0.85c	4.24±0.40c

Effects of Exogenous H₂O₂ on Physiological Characteristics of Populus tomentosa Under Saline-alkali Stress

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处理	7月4日		7月16日
处理	AsA	GSH	ASA	GSH
H1(CK)	5.12±0.30c	0.18±0.02b	5.75±0.70c	0.21±0.02b
H2	5.37±0.40bc	0.26±0.04a	6.03±0.50bc	0.31±0.04a
H3	6.14±0.33b	0.24±0.02a	6.89±0.80ab	0.28±0.03a
H4	7.52±0.50a	0.12±0.01c	7.97±0.90a	0.17±0.02b

处理	7月4日			7月16日
处理	可溶性糖含量	可溶性蛋白	游离脯氨酸	可溶性糖含量	可溶性蛋白	游离脯氨酸
H1(CK)	56.47±3.17d	3.23±0.20c	13.69±0.95c	68.97±1.98b	4.28±0.24b	15.76±1.70c
H2	153.21±11.76a	4.69±0.61b	17.56±2.01b	179.79±20.09a	5.25± 0.39a	19.33±2.00b
H3	123.39±10.03b	6.35±0.58a	23.41±2.91a	152.52±12.37a	4.69±0.60ab	27.44±2.40a
H4	71.39±3.89c	5.15±0.40b	16.12±1.00b	78.55±6.21b	4.49±0.41ab	20.33±1.40b

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