Exogenous Zinc: Effects on the Growth and Antioxidant System of Coix lachryma-jobi L. Under Nitrogen Stress

doi:10.11924/j.issn.1000-6850.casb2021-0221

Abstract

Abstract:

The study aims to alleviate the injury of nitrogen stress on Coix lachryma-jobi L. and provide a reference basis for its cultivation in nitrogen-deficiency areas. Taking the seeds of Coix lachryma-jobi L. in southwest Guizhou as test materials, pot experiment method was used to study the effects of exogenous zinc and nitrogen stress on plant growth, chlorophyll content, biomass accumulation and distribution, peroxidase (POD) and catalase (CAT) activities, and malondialdehyde (MDA) content of Coix lachryma-jobi L. seedlings. The results showed that: (1) the plant height, root length, main root number, total leaf number and biomass of each part of Coix lachryma-jobi L. seedlings decreased significantly under nitrogen deficiency (P<0.05, the same below), which decreased the total biomass by 80.65% compared with that of the control; the activities of CAT and POD were significantly inhibited, and the content of MDA significantly increased by 26.29%; (2) zinc application significantly increased the plant height, leaf area and stem biomass of Coix lachryma-jobi L.; the chlorophyll content showed an overall upward trend, and the CAT and POD activities significantly increased; at the same time, the root system was more sensitive to exogenous zinc, and the root length significantly decreased by 28.09%, while the number of tap roots increased by 16.86%; (3) zinc application and nitrogen deficiency significantly increased the plant height and root length of Coix lachryma-jobi L. seedlings by 20.30% and 17.72%, respectively; the antioxidant enzyme CAT activity significantly increased by 171.01%, and there was no significant change in POD activity and MDA accumulation. This study indicates that zinc application under nitrogen stress could promote the growth of Coix lachryma-jobi L. seedlings, balance the seedlings’ antioxidant system and improve their adaptability, thus alleviating the adverse effects caused by nitrogen stress to a certain extent.

Key words: Coix lachryma-jobi L., nitrogen stress, exogenous zinc, seedling growth, antioxidant system

CLC Number:

XIONG Lulu, LU Youzuo, LIU Yueyan, PENG Hailan, HUANG Xinmin, WANG Jianjian. Exogenous Zinc: Effects on the Growth and Antioxidant System of Coix lachryma-jobi L. Under Nitrogen Stress[J]. Chinese Agricultural Science Bulletin, 2021, 37(36): 24-29.

Figures/Tables 5

References 32

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处理	株高/cm	根长/cm	主根数/条	总叶片数/片	比叶面积/cm²
CK	66.69±0.41c	38.80±0.46c	9.67±0.27b	13.34±0.62b	86.56±1.08b
Zn	70.07±1.06d	27.90±0.72a	11.3±0.03c	12.29±0.47b	103.20±0.38c
-N	29.41±0.59a	27.03±0.15a	5.83±0.40a	6.00±0.00a	24.45±0.27a
-N+Zn	35.38±0.21b	31.82±0.51b	6.25±0.43a	6.33±0.25a	25.64±0.50a

处理	株高/cm	根长/cm	主根数/条	总叶片数/片	比叶面积/cm²
CK	66.69±0.41c	38.80±0.46c	9.67±0.27b	13.34±0.62b	86.56±1.08b
Zn	70.07±1.06d	27.90±0.72a	11.3±0.03c	12.29±0.47b	103.20±0.38c
-N	29.41±0.59a	27.03±0.15a	5.83±0.40a	6.00±0.00a	24.45±0.27a
-N+Zn	35.38±0.21b	31.82±0.51b	6.25±0.43a	6.33±0.25a	25.64±0.50a

处理	根生物量/g	茎生物量/g	叶生物量/g	总生物量/g	根冠比
CK	0.23±0.05b	0.22±0.01b	1.72±0.07c	2.17±0.10b	0.12±0.04a
Zn	0.25±0.08b	0.52±0.06c	1.48±0.09b	2.25±0.0.2b	0.12±0.01a
-N	0.09±0.02a	0.03±0.00a	0.31±0.04a	0.42±0.10a	0.26±0.03b
-N+Zn	0.09±0.04a	0.06±0.02a	0.30±0.02a	0.44±0.06a	0.26±0.02b

处理	根生物量/g	茎生物量/g	叶生物量/g	总生物量/g	根冠比
CK	0.23±0.05b	0.22±0.01b	1.72±0.07c	2.17±0.10b	0.12±0.04a
Zn	0.25±0.08b	0.52±0.06c	1.48±0.09b	2.25±0.0.2b	0.12±0.01a
-N	0.09±0.02a	0.03±0.00a	0.31±0.04a	0.42±0.10a	0.26±0.03b
-N+Zn	0.09±0.04a	0.06±0.02a	0.30±0.02a	0.44±0.06a	0.26±0.02b