Exogenous Glutathione: Effects on Maize Seedlings Under Low Temperature Stress

doi:10.11924/j.issn.1000-6850.casb2020-0764

Abstract

Abstract:

The purpose of this experiment is to study the effect of exogenous glutathione on enhancing the defense of corn seedlings against low temperature stress. The maize variety ‘Zhengdan 958’ was used as material in this study. Soil culture method was carried out with six treatments (CK, LT, G1, G2, G3 and G4), and each treatment was repeated three times. The maize seedlings with three leaves had leaf-spray for 3 days, and then transferred to artificial climate box with 10℃ low temperature stress. The growth traits of maize seedlings and the related physiological indexes were analyzed. The results show that spraying exogenous glutathione could significantly reduce the effects of low temperature stress on maize seedling height, leaf water content and chlorophyll content. Glutathione could alleviate the oxidation damage of maize seedlings under low temperature stress through enhancing the activity of antioxidant enzymes. There is ignificant difference between LT and G3 whose antioxidant enzyme activities are increased by 79.25%. With the increase of low temperature stress days, the contents of soluble sugar and malondialdehyde in maize leaves increase gradually. Compared with the fifth day after stress, the contents of soluble sugar and malondialdehyde on the seventh day are increased by 106.97% and 25.33%, respectively. Taken together, exogenous glutathione can improve the growth ability of maize under low temperature stress, mainly by increasing the activity of antioxidant enzymes and photosynthetic regulation ability, and maintaining cell osmotic balance.

Key words: maize, low temperature stress, exogenous glutathione, seedling growth, physiological characteristics

CLC Number:

S513

Li Lin, Du Zhuo, Hou Wen, Zhang Kai, Huang Hao, Lu Yuncai. Exogenous Glutathione: Effects on Maize Seedlings Under Low Temperature Stress[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 16-20.

Figures/Tables 6

References 28

[1]	孙玉珺. 玉米芽期抗冷性筛选及低温胁迫下油菜素内酯对幼苗的调控效应研究[D]. 哈尔滨:东北农业大学, 2019:1-2.
[2]	Gong F P, Yang L, Tai F J, et al. “Omics” of maize stress response for sustainable food production: opportunities and challenges[J]. Omics A Journal of Integrative Biology, 2014, 18(12):714-732. doi: 10.1089/omi.2014.0125 URL
[3]	侯雯, 杜卓, 王丽, 等. 外源褪黑素对低温胁迫下玉米幼苗生长和生理特性的影响[J]. 中国糖料, 2020, 42(02):33-37.
[4]	Farooqi M, Sa K, Hong T, et al. Bulk Segregant Analysis (BSA) for improving cold stress resistance in maize using SSR markers[J]. Genetics & Molecular Research Gmr, 2016, 15(4):1-12.
[5]	Li Z, Xu J K, Gao Y, et al. The synergistic priming effect of exogenous salicylic acid and H₂O₂ on chilling tolerance enhancement during maize (Zea mays L.) seed germination[J]. Frontiers in Plant Science, 2017, 8:1153. doi: 10.3389/fpls.2017.01153 URL
[6]	孙磊, 王丽华, 高中超, 等. 黑龙江省玉米低温冷害致灾机理及防御措施[J]. 黑龙江农业科学, 2019(11):148-153.
[7]	吕天放, 徐田军, 刘月娥, 等. 低温胁迫对不同基因型玉米种子萌发特性的影响[J]. 玉米科学, 2018, 26(06):45-49.
[8]	杜卓, 侯雯, 王丽, 等. 外源褪黑素对干旱胁迫下玉米幼苗的影响[J]. 中国农学通报, 2020, 36(27):14-19.
[9]	杨志峰, 王小宇, 崔金霞, 等. 低温胁迫下外源NO与GSH协同作用提高黄瓜幼苗耐冷性[J]. 植物生理学报, 2020, 56(7):1573-1582.
[10]	陈少裕. 植物谷胱甘肽的生理作用及其意义[J]. 植物生理学通讯, 1993, 29(3):210-214.
[11]	周艳, 刘慧英, 王松, 等. 外源GSH对盐胁迫下番茄幼苗生长及抗逆生理指标的影响[J]. 西北植物学报, 2016, 36(3):515-520.
[12]	周艳, 刘慧英, 崔金霞, 等. 外源GSH对NaCl胁迫下番茄幼苗叶片及根系离子微域分布的影响[J]. 植物营养与肥料学报, 2017, 23(4):964-972.
[13]	丁继军, 刘柿良, 潘远智, 等. 外源AsA、GSH对Cd胁迫下石竹幼苗生长的影响[J]. 应用生态学报, 2014, 25(2):419-426.
[14]	Hussain B M, Akram S, Raffi S A, et al. Exogenous glutathione improves salinity stress tolerance in rice (Oryza sativa L.)[J]. Plant Gene and Trait, 2016, 7:0011.
[15]	于威, 颉建明, 滕汉玮. 外源谷胱甘肽对嫁接黄瓜砧穗种子萌发过程中自毒胁迫的缓解效应[J]. 核农学报, 2016, 30(08):1633-1638.
[16]	Ibrahim W, Ahmed I M, Chen X, et al. Genotype-dependent alleviation effects of exogenous GSH on salinity stress in cotton is related to improvement in chlorophyll content, photosynthetic performance, and leaf/root ultrastructure[J]. Environmental Science and Pollution Research, 2017, 24(10):9417-9427. doi: 10.1007/s11356-017-8611-7 URL
[17]	邹琦. 植物生理学实验指导[M]. 北京: 中国农学大学出版社, 2000:203-229.
[18]	赵平. 不同基因型小麦抗旱性差异研究[D]. 合肥:安徽农业大学, 2011:12-13.
[19]	王学奎, 黄见良. 植物生理生化实验原理与技术[M]. 北京: 高等教育出版社, 2015:276-277.
[20]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000:171-174.
[21]	索琳格, 崔金霞, 吴佩, 等. 内源GSH参与低温胁迫下外源NO对黄瓜幼苗叶片光合作用的调控[J]. 西北植物学报, 2019, 39(12):2207-2217.
[22]	单长卷, 代海芳. 外源谷胱甘肽对干旱胁迫下玉米幼苗叶片生理特性的影响[J]. 灌溉排水学报, 2016, 35(01):59-62.
[23]	Zhou Y, Diao M, Cui J, et al. Exogenous GSH protects tomatoes against salt stress by modulating photosystem Ⅱ efficiency, absorbed light allocation and H₂O₂-scavenging system in chloroplasts[J]. Journal of Integrative Agriculture, 2018, 17(10):2257-2272. doi: 10.1016/S2095-3119(18)62068-4 URL
[24]	田娜, 王辉, 米乐, 等. 外源谷胱甘肽对苯丙烯酸胁迫下黄瓜幼苗抗氧化酶活性的影响[J]. 贵州农业科学, 2013, 41(04):37-39.
[25]	孙丽. 外源硒对低温胁迫下草莓幼苗的缓解效应及对AsA-GSH循环的影响[D]. 杭州:浙江大学, 2016:18-20.
[26]	单长卷, 杨天佑. 谷胱甘肽对盐胁迫下玉米幼苗抗氧化特性和光合性能的影响[J]. 西北农业学报, 2017, 26(02):185-191.
[27]	王绍娴. 外源谷胱甘肽对番茄体内抗氧化系统的影响[D]. 哈尔滨:东北农业大学, 2016:17-36.
[28]	温泽林. 外源GSH介导NO调控番茄幼苗盐适应性研究[D]. 石河子:石河子大学, 2018:13-20.

处理条件	苗高/cm
处理条件	0 d	3 d	5 d	7 d
CK	35.83±0.40a	43.23±0.21a	45.13±0.25a	47.77±0.25a
LT	35.97±0.61a	37.23±0.25d	37.60±0.36d	37.83±0.42d
G1	35.80±0.10a	40.53±0.25b	40.77±0.25b	41.00±0.27b
G2	36.17±0.25a	39.40±1.23c	40.50±0.87bc	40.50±0.87bc
G3	35.45±0.44a	38.57±0.21e	39.07±0.38e	39.30±0.61e
G4	35.73±0.15a	39.63±0.32bc	39.73±0.32c	39.83±0.31c

处理条件	苗高/cm
处理条件	0 d	3 d	5 d	7 d
CK	35.83±0.40a	43.23±0.21a	45.13±0.25a	47.77±0.25a
LT	35.97±0.61a	37.23±0.25d	37.60±0.36d	37.83±0.42d
G1	35.80±0.10a	40.53±0.25b	40.77±0.25b	41.00±0.27b
G2	36.17±0.25a	39.40±1.23c	40.50±0.87bc	40.50±0.87bc
G3	35.45±0.44a	38.57±0.21e	39.07±0.38e	39.30±0.61e
G4	35.73±0.15a	39.63±0.32bc	39.73±0.32c	39.83±0.31c

处理条件	叶片含水量/%
处理条件	0 d	3 d	5 d	7 d
CK	90.40±0.03a	85.63±0.29a	82.68±0.15a	81.40±0.18ab
LT	90.13±0.16a	84.85±0.38a	81.69±3.63a	80.48±2.14bc
G1	90.17±0.03a	85.54±0.47a	82.98±0.67a	82.23±2.38ab
G2	90.08±0.08a	85.89±0.77a	82.93±1.37a	81.55±0.46ab
G3	90.10±0.01a	86.52±0.63a	84.25±0.38a	83.47±0.28a
G4	90.08±0.08a	85.67±2.56a	82.30±3.05a	83.00±1.55a

处理条件	叶片含水量/%
处理条件	0 d	3 d	5 d	7 d
CK	90.40±0.03a	85.63±0.29a	82.68±0.15a	81.40±0.18ab
LT	90.13±0.16a	84.85±0.38a	81.69±3.63a	80.48±2.14bc
G1	90.17±0.03a	85.54±0.47a	82.98±0.67a	82.23±2.38ab
G2	90.08±0.08a	85.89±0.77a	82.93±1.37a	81.55±0.46ab
G3	90.10±0.01a	86.52±0.63a	84.25±0.38a	83.47±0.28a
G4	90.08±0.08a	85.67±2.56a	82.30±3.05a	83.00±1.55a