Ultra-structural Characteristics of the Antennal Sensilla of Two Species of Caloptilia (Lepidoptera: Gracillariidae)

doi:10.11924/j.issn.1000-6850.casb2021-0505

Abstract

Abstract:

Caloptilia theivora and Caloptilia kurokoi are the leaf-miners of some plants respectively belonging to the family of Theaceae and Aceraceae. The study aims to understand the types, quantities, distribution and morphological characteristics of the antennal sensilla of the two species of Caloptilia, and explore their intraspecific and interspecific differences of antennal sensilla characteristics. Scanning electron microscope was used for the analysis. There are 11 types and 16 subtypes of antennal sensilla of the two species of Caloptilia, including sensilla trichodea (2 subtypes), sensilla chaetica (2 subtypes), sensilla coeloclnica (2 subtypes), sensilla styloconica (3 subtypes), sensilla auriculate (3 subtypes), sensilla basiconca, sensilla squamiformia (2 subtypes), sensilla intersegment (2 subtypes), Böhm bristles, sensilla mammilliformia and sensilla lamellar spiny. The female Caloptilia theivora has 11 types of sensilla (11 subtypes) and the male one has 10 types (13 subtypes). Both female and male Caloptilia kurokoi have 10 types and 13 subtypes of antennal sensilla. Semifusion was observed in the first subsegment of the flagellum of the female Caloptilia theivora. Both the two species of Caloptilia have sexual dimorphism in antennal sensilla, and there are some interspecific differences. Sensilla intersegment, sensilla mammilliformia and sensilla lamellar spiny are first reported in Lepidopteran. The antennal sensilla types of Gracillariidae and even Lepidoptera are greatly enriched by the research, which can lay a basis for further exploring the phylogenetic development, behaviors and pest and insect control of Gracillariidae.

Key words: antennal sensilla, sexual dimorphism, Caloptilia, Gracillariidae, scanning electron microscope

CLC Number:

S476

LIANG Jingxuan, BAI Haiyan. Ultra-structural Characteristics of the Antennal Sensilla of Two Species of Caloptilia (Lepidoptera: Gracillariidae)[J]. Chinese Agricultural Science Bulletin, 2022, 38(16): 110-118.

Figures/Tables 4

References 33

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触角形态	茶丽细蛾		黑丽细蛾
触角形态	雌	雄	雌	雄
柄节	201.67±5.93a	232.17±14.90ab	264.33±20.99b	191.67±5.74bc
梗	83.53±2.77a	84.75±1.44a	85.93±2.46a	83.00±1.23a
鞭节	6827.00±175.89a	6236.13±135.14b	6873.00±74.88b	7523.00±74.88c
全长	7157.2±220.94a	6553.05±127.08b	7223.27±70.28b	7797.67±60.58c

触角形态	茶丽细蛾		黑丽细蛾
触角形态	雌	雄	雌	雄
柄节	201.67±5.93a	232.17±14.90ab	264.33±20.99b	191.67±5.74bc
梗	83.53±2.77a	84.75±1.44a	85.93±2.46a	83.00±1.23a
鞭节	6827.00±175.89a	6236.13±135.14b	6873.00±74.88b	7523.00±74.88c
全长	7157.2±220.94a	6553.05±127.08b	7223.27±70.28b	7797.67±60.58c

感器类型		性别	茶丽细蛾				黑丽细蛾
感器类型		性别	数量	长度/μm	基部直径/μm	臼状窝直径/μm	数量	长度/μm	基部直径/μm	臼状窝直径/μm
Str	Str I	雌	2326.67±177.81a	29.25±1.35a	1.44±0.11a	3.42±0.16a	3056.17±149.20b	32.02±2.50b	1.72±0.49a	3.35±0.56b
	Str I	雄	2137.33±159.47a	33.10±1.37a	1.37±0.08a	2.57±0.15ab	3724.50±212.75b	39.57±4.05a	1.73±0.14a	3.25±0.48b
	Str II	雌	511.67±45.62a	12.41±0.85a	1.67±0.13a	3.10±0.55a	606.17±38.76b	21.03±2.16b	1.38±0.33a	3.34±0.36a
	Str II	雄	411.33±35.81a	15.45±0.69a	1.34±0.15a	2.83±0.18a	765.83±102.88ab	22.24±3.06b	1.46±0.26a	2.90±0.37a
Sch	Sch I	雌	135.00±4.53a	13.46±0.54a	0.94±0.10a	2.29±0.16a	152.00±6.57c	18.03±0.43b	1.13±0.06a	2.36±0.10a
	Sch I	雄	101.50±3.53ab	12.81±0.48a	1.16±0.05a	2.54±0.17a	115.67±4.10bc	14.72±0.59a	1.08±0.05a	2.08±0.09a
	Sch II	雌	26.00±2.24a	7.63±0.41a	0.86±0.04a	2.23±0.09a	27.83±2.70a	13.06±0.54b	1.04±0.05b	2.26±0.10b
	Sch II	雄	22.50±0.76a	8.75±0.47a	1.12±0.03ab	2.76±0.10a	26.00±3.29a	9.09±0.32a	1.06±0.11ab	2.17±0.17a
Sco	Sco I	雌	51.83±2.68a	5.19±0.30a	—	5.65±0.21a	70.33±3.03c	6.05±0.38b	—	6.31±0.29a
	Sco I	雄	58.83±2.33a	6.33±0.31ab	—	6.16±1.37a	82.50±2.45b	4.34±0.23b	—	6.12±0.27a
	Sco II	雌	—	—	—	—	—	—	—	—
	Sco II	雄	—	—	—	—	8.17±2.41	5.03±0.30	—	11.64±0.53
Sst	Sst I	雌	50.83±3.40	9.52±1.54	3.01±0.65	—	—	—	—	—
	Sst I	雄	—	—	—	—	—	—	—	—
	Sst II	雌	—	—	—	—	66.00±3.29b	10.24±0.69b	3.89±0.20b	—
	Sst II	雄	47.83±2.30a	8.91±1.36a	2.72±0.64a	—	71.33±1.61b	9.38±0.10a	3.65±0.61a	—
	Sst III	雌	—	—	—	—	—	—	—	—
	Sst III	雄	—	—	—	—	4.75±0.48	11.94±0.57	3.17±0.25	—
Sau	Sau I	雌	—	—	—		36.17±3.29c	24.55±1.65b	—	—
	Sau I	雄	19.50±1.88a	17.95±2.18a	—	—	113.00±2.54b	15.84±0.42a	—	—
	Sau II	雌	86.17±3.74a	16.26±0.46a	—	—	21.33±3.14c	21.27±1.83b	—	—
	Sau II	雄	57.83±2.30b	17.58±0.95a	—	—	—	—	—	—
	Sau III	雌	156.83±6.36a	23.28±0.98a	—	—	86.00±3.29b	25.15±0.55b	—	—
	Sau III	雄	97.83±2.30a	21.64±0.89ab	—	—	101.17±1.58a	20.10±0.94bc	—	—
Sba	Sba	雌	26.83±2.73a	11.08±0.15a	1.48±0.05a	—	35.50±2.93b	21.97±1.54c	4.37±0.13b	—
Sba	Sba	雄	21.83±1.17ab	20.37±0.15b	1.38±0.09ab	—	27.00±2.19b	15.81±1.09c	1.70±0.07ab	—
Sin	Sin I	雌	1	10.63±0.18	29.01±0.37	—	1	41.67±0.88	16.30±0.15	—
	Sin I	雄	1	6.99±0.88	20.72±0.16	—	1	46.00±2.64	18.90±1.35	—
	Sin II	雌	9.67±0.20a	1.71±0.14a	1.57±0.32a	—	9.00±2.00a	2.01±0.15a	1.91±0.49a	—
	Sin II	雄	6.00±1.00a	2.06±0.06a	1.94±0.05a	—	11.33±2.03a	1.92±0.11a	1.76±0.10a	—
Ssq	Ssq I	雌	—	—	—	—	3.83±0.59	27.18±2.55	—	—
	Ssq I	雄	2.50±0.62	18.13±1.61	—	—	—	—	—	—
	Ssq II	雌	1083.33±70.58a	18.55±0.43a	—	—	1209.33±51.83b	22.21±0.37b	—	—
	Ssq II	雄	1018.33±33.70a	20.25±0.59ab	—	—	1520.00±51.38a	20.88±0.84b	—	—
Böhm	Bbr	雌	80.53±3.51a	7.02±1.21a	1.05±0.10a	—	65.67±3.56b	4.41±0.24a	1.27±0.06a	—
Böhm	Bbr	雄	61.67±5.30ab	8.82±1.83a	1.23±0.09a	—	85.67±2.85bc	7.65±0.82a	1.39±0.10a	—
Sma	Sma	雌	3.80±0.58	3.40±0.83	2.48±0.34	—	—	—	—	—
Sma	Sma	雄	—	—	—	—	—	—	—	—

感器类型		性别	茶丽细蛾				黑丽细蛾
感器类型		性别	数量	长度/μm	基部直径/μm	臼状窝直径/μm	数量	长度/μm	基部直径/μm	臼状窝直径/μm
Str	Str I	雌	2326.67±177.81a	29.25±1.35a	1.44±0.11a	3.42±0.16a	3056.17±149.20b	32.02±2.50b	1.72±0.49a	3.35±0.56b
	Str I	雄	2137.33±159.47a	33.10±1.37a	1.37±0.08a	2.57±0.15ab	3724.50±212.75b	39.57±4.05a	1.73±0.14a	3.25±0.48b
	Str II	雌	511.67±45.62a	12.41±0.85a	1.67±0.13a	3.10±0.55a	606.17±38.76b	21.03±2.16b	1.38±0.33a	3.34±0.36a
	Str II	雄	411.33±35.81a	15.45±0.69a	1.34±0.15a	2.83±0.18a	765.83±102.88ab	22.24±3.06b	1.46±0.26a	2.90±0.37a
Sch	Sch I	雌	135.00±4.53a	13.46±0.54a	0.94±0.10a	2.29±0.16a	152.00±6.57c	18.03±0.43b	1.13±0.06a	2.36±0.10a
	Sch I	雄	101.50±3.53ab	12.81±0.48a	1.16±0.05a	2.54±0.17a	115.67±4.10bc	14.72±0.59a	1.08±0.05a	2.08±0.09a
	Sch II	雌	26.00±2.24a	7.63±0.41a	0.86±0.04a	2.23±0.09a	27.83±2.70a	13.06±0.54b	1.04±0.05b	2.26±0.10b
	Sch II	雄	22.50±0.76a	8.75±0.47a	1.12±0.03ab	2.76±0.10a	26.00±3.29a	9.09±0.32a	1.06±0.11ab	2.17±0.17a
Sco	Sco I	雌	51.83±2.68a	5.19±0.30a	—	5.65±0.21a	70.33±3.03c	6.05±0.38b	—	6.31±0.29a
	Sco I	雄	58.83±2.33a	6.33±0.31ab	—	6.16±1.37a	82.50±2.45b	4.34±0.23b	—	6.12±0.27a
	Sco II	雌	—	—	—	—	—	—	—	—
	Sco II	雄	—	—	—	—	8.17±2.41	5.03±0.30	—	11.64±0.53
Sst	Sst I	雌	50.83±3.40	9.52±1.54	3.01±0.65	—	—	—	—	—
	Sst I	雄	—	—	—	—	—	—	—	—
	Sst II	雌	—	—	—	—	66.00±3.29b	10.24±0.69b	3.89±0.20b	—
	Sst II	雄	47.83±2.30a	8.91±1.36a	2.72±0.64a	—	71.33±1.61b	9.38±0.10a	3.65±0.61a	—
	Sst III	雌	—	—	—	—	—	—	—	—
	Sst III	雄	—	—	—	—	4.75±0.48	11.94±0.57	3.17±0.25	—
Sau	Sau I	雌	—	—	—		36.17±3.29c	24.55±1.65b	—	—
	Sau I	雄	19.50±1.88a	17.95±2.18a	—	—	113.00±2.54b	15.84±0.42a	—	—
	Sau II	雌	86.17±3.74a	16.26±0.46a	—	—	21.33±3.14c	21.27±1.83b	—	—
	Sau II	雄	57.83±2.30b	17.58±0.95a	—	—	—	—	—	—
	Sau III	雌	156.83±6.36a	23.28±0.98a	—	—	86.00±3.29b	25.15±0.55b	—	—
	Sau III	雄	97.83±2.30a	21.64±0.89ab	—	—	101.17±1.58a	20.10±0.94bc	—	—
Sba	Sba	雌	26.83±2.73a	11.08±0.15a	1.48±0.05a	—	35.50±2.93b	21.97±1.54c	4.37±0.13b	—
Sba	Sba	雄	21.83±1.17ab	20.37±0.15b	1.38±0.09ab	—	27.00±2.19b	15.81±1.09c	1.70±0.07ab	—
Sin	Sin I	雌	1	10.63±0.18	29.01±0.37	—	1	41.67±0.88	16.30±0.15	—
	Sin I	雄	1	6.99±0.88	20.72±0.16	—	1	46.00±2.64	18.90±1.35	—
	Sin II	雌	9.67±0.20a	1.71±0.14a	1.57±0.32a	—	9.00±2.00a	2.01±0.15a	1.91±0.49a	—
	Sin II	雄	6.00±1.00a	2.06±0.06a	1.94±0.05a	—	11.33±2.03a	1.92±0.11a	1.76±0.10a	—
Ssq	Ssq I	雌	—	—	—	—	3.83±0.59	27.18±2.55	—	—
	Ssq I	雄	2.50±0.62	18.13±1.61	—	—	—	—	—	—
	Ssq II	雌	1083.33±70.58a	18.55±0.43a	—	—	1209.33±51.83b	22.21±0.37b	—	—
	Ssq II	雄	1018.33±33.70a	20.25±0.59ab	—	—	1520.00±51.38a	20.88±0.84b	—	—
Böhm	Bbr	雌	80.53±3.51a	7.02±1.21a	1.05±0.10a	—	65.67±3.56b	4.41±0.24a	1.27±0.06a	—
Böhm	Bbr	雄	61.67±5.30ab	8.82±1.83a	1.23±0.09a	—	85.67±2.85bc	7.65±0.82a	1.39±0.10a	—
Sma	Sma	雌	3.80±0.58	3.40±0.83	2.48±0.34	—	—	—	—	—
Sma	Sma	雄	—	—	—	—	—	—	—	—