Transcription Factor BvMYB44 Gene in Energy Beet: Expression Characteristics in Response to Cadmium Stress and Its Bioinformatics Analysis

doi:10.11924/j.issn.1000-6850.casb2021-0677

Abstract

Abstract:

In order to analyze the regulatory role of BvMYB44 of energy beet under heavy metal stress, this study used ‘780016B/12You’ as material to study the expression pattern of BvMYB44 in response to cadmium stress by qRT-PCR, and analyzed its function by bioinformatics. The results show that the total length of cDNA of BvMYB44 gene is 942 bp, encoding 313 amino acids, the molecular weight of the protein is 33935.01 Da, and the theoretical isoelectric point is 7.57. BvMYB44 is an unstable alkaline hydrophilic protein with 39 phosphorylation sites located in the nucleus and only one exon. BvMYB44 is a member of the R2R3-MYB subfamily and is most closely related to CqMYB44-like from Chenopodium quinoa and SoMYB44-like from Spinacia oleracea. Several cis-acting elements related to abiotic stress are predicted in the promoter region of this gene, which is speculated to be involved in a variety of abiotic stress responses. The qRT-PCR shows that BvMYB44 has different levels of significantly up-regulated expression in energy beet leaves and roots under 0.5 mmol/L cadmium stress. Therefore, it can be inferred that BvMYB44 has a certain response relationship with cadmium stress. This study will provide candidate genes and a theoretical basis for germplasm innovation of heavy metal-resistant energy beet and expansion of plant remediation.

Key words: energy beet, BvMYB44, bioinformatics, cadmium stress, expression analysis

CLC Number:

S566.3

Wang Man, Zhou Wanting, Zhou Xiang, Li Siqi, Wang Xueqian, Wang Luhong, Li Wangsheng, Li Jiajia, Gao Zhuo, Liu Dali. Transcription Factor BvMYB44 Gene in Energy Beet: Expression Characteristics in Response to Cadmium Stress and Its Bioinformatics Analysis[J]. Chinese Agricultural Science Bulletin, 2021, 37(35): 25-33.

Figures/Tables 10

References 31

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元件名称	位置	序列	功能
ABRE	+216,-984	GCAACGTGTC,ACGTG	参与脱落酸响应的顺式作用元件
CAAT-box	+8,-178,+89,-473,+87,+266,+150,-522,+37,-104,+88, -167,-186,-444,+392,-531,-570,+598,-609,+667,-670, -713,+718,+727,-732,-734,+764,-769,-771,+845,-899, +1015,-1017,+1152,+1180,+1202,-1233,+1264,-1277, -1282,-1333,+1349,+1354,+1364,+1379,-1413,+1484, +1549,+1550,-1552,+1667,+1771,+1781	CAAAT,CAAT,CCCAATTT,CCAAT	转录增强元件
CGTCA-motif	-1791	CGTCA	参与茉莉酸甲酯响应的顺式作用元件
ERE	-1092,+1090	ATTTTAAA	乙烯响应元件
MYB	-1554,-1145,+1850	CAACCA,TAACCA	干旱响应元件
MYC	-1550,+1412,-1264,-964,-1202,-718,+452	CAATTG,CATTTG,CATGTG	缺水、抗脱落酸、抗冻、抗寒响应元件
P-box	+792	CCTTTTG	赤霉素响应元件
STRE	-1993,-1915,-1891	AGGGG	胁迫响应元件
TATA-box	-124,+701,+330,-1235,+141,-1086,+626,-1659,-130,+911,+378,-1633,+290,-1110,+642,-1975,+338,+909,+627, +1570,+431,-1108,+700,-1767,+126,+1085,+269,-1634, +140,-1155,+289,-2766,+337,+702,+528,-1237,-123, -1107,+234,+1873,-377,+988,+129,+1632,+,628,-1109, +257,-910,-125,-1029,+131	TATAAA,ATATAT,TATA,TATACA, TATATA,TATAA,TACAAAA, ATTATA,ccTATAAAaa,TATAAAA	核心启动元件
TATC-box	+1816	TATCCCA	参与赤霉素响应的顺式作用元件
TGACG-motif	+1791	TGACG	参与茉莉酸甲酯响应的顺式作用元件
W box	-63	TTGACC	参与防御和胁迫反应的顺式作用元件
WUN-motif	-1250	TTATTACAT	伤害响应元件
as-1	+1791	TGACG	伤害、赤霉素、茉莉酸甲酯响应元件

元件名称	位置	序列	功能
ABRE	+216,-984	GCAACGTGTC,ACGTG	参与脱落酸响应的顺式作用元件
CAAT-box	+8,-178,+89,-473,+87,+266,+150,-522,+37,-104,+88, -167,-186,-444,+392,-531,-570,+598,-609,+667,-670, -713,+718,+727,-732,-734,+764,-769,-771,+845,-899, +1015,-1017,+1152,+1180,+1202,-1233,+1264,-1277, -1282,-1333,+1349,+1354,+1364,+1379,-1413,+1484, +1549,+1550,-1552,+1667,+1771,+1781	CAAAT,CAAT,CCCAATTT,CCAAT	转录增强元件
CGTCA-motif	-1791	CGTCA	参与茉莉酸甲酯响应的顺式作用元件
ERE	-1092,+1090	ATTTTAAA	乙烯响应元件
MYB	-1554,-1145,+1850	CAACCA,TAACCA	干旱响应元件
MYC	-1550,+1412,-1264,-964,-1202,-718,+452	CAATTG,CATTTG,CATGTG	缺水、抗脱落酸、抗冻、抗寒响应元件
P-box	+792	CCTTTTG	赤霉素响应元件
STRE	-1993,-1915,-1891	AGGGG	胁迫响应元件
TATA-box	-124,+701,+330,-1235,+141,-1086,+626,-1659,-130,+911,+378,-1633,+290,-1110,+642,-1975,+338,+909,+627, +1570,+431,-1108,+700,-1767,+126,+1085,+269,-1634, +140,-1155,+289,-2766,+337,+702,+528,-1237,-123, -1107,+234,+1873,-377,+988,+129,+1632,+,628,-1109, +257,-910,-125,-1029,+131	TATAAA,ATATAT,TATA,TATACA, TATATA,TATAA,TACAAAA, ATTATA,ccTATAAAaa,TATAAAA	核心启动元件
TATC-box	+1816	TATCCCA	参与赤霉素响应的顺式作用元件
TGACG-motif	+1791	TGACG	参与茉莉酸甲酯响应的顺式作用元件
W box	-63	TTGACC	参与防御和胁迫反应的顺式作用元件
WUN-motif	-1250	TTATTACAT	伤害响应元件
as-1	+1791	TGACG	伤害、赤霉素、茉莉酸甲酯响应元件