ARF Gene Family in Sugar Beet: Bioinformatics Analysis and Differential Expression in Seed Germination Period

doi:10.11924/j.issn.1000-6850.casb20200100037

Abstract

Abstract:

The purpose is to understand the expression difference of ARF gene family in sugarbeet seeds in germination period and under the condition of drought environment. The transcriptome sequencing of sugarbeet samples in three germination stages (dry seeds, seed shell cracking, dew white) was carried out in the treatment group (15% PEG simulated drought stress) and the control group (distilled water). The evolution of ARF gene system, conserved domain of protein, chromosome distribution and differential expression under drought were analyzed by bioinformatics. Amino acid sequence analysis showed that ARF transcription factors contained 8 conserved elements, and the number of amino acid sites in the domain of 8 conserved elements was not consistent. It was speculated that ARF transcription factors were related to the binding and function of corresponding DNA molecules and distributed unevenly on chromosomes. The results of transcriptome sequencing showed that the expression of EntrezID-104886784, 104903260, 104908672 genes increased gradually in the three stages of dry seed, seed shell cracking and dew white in contrast and drought conditions, and the trend was obvious. Under the condition of drought stress, the expression of EntrezID-104886784 increased the most significantly, followed by that of EntrezID-104903260, and the expression of EntrezID-104908672 increased less; in the control, the expression of EntrezID-104908672 and EntrezID-104886784 increased significantly. However, the expression level of EntrezID-104903260 increased most obviously when it was exposed. There are 14 ARF transcription factors in sugarbeet genome, three of which are differentially expressed under drought stress, and it is predicted that drought condition will significantly affect the two genes of EntrezID-104908672 and EntrezID-104903260.

Key words: sugarbeet, ARF gene family, bioinformatics analysis, drought stress, germination period

CLC Number:

S566.3

Liu Naixin, Wu Yumei. ARF Gene Family in Sugar Beet: Bioinformatics Analysis and Differential Expression in Seed Germination Period[J]. Chinese Agricultural Science Bulletin, 2020, 36(26): 22-28.

Figures/Tables 7

References 45

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候选基因	候选基因
EntrezID_104886784	EntrezID_104901644
EntrezID_104889090	EntrezID_104902645
EntrezID_104891238	EntrezID_104903260
EntrezID_104893911	EntrezID_104905779
EntrezID_104897022	EntrezID_104906353
EntrezID_104898663	EntrezID_104908192
EntrezID_104900616	EntrezID_104908672

候选基因	候选基因
EntrezID_104886784	EntrezID_104901644
EntrezID_104889090	EntrezID_104902645
EntrezID_104891238	EntrezID_104903260
EntrezID_104893911	EntrezID_104905779
EntrezID_104897022	EntrezID_104906353
EntrezID_104898663	EntrezID_104908192
EntrezID_104900616	EntrezID_104908672