Abstract:

The present study is focusing on the soil bacterial community structure and diversity of Morchella inferred from the high-throughput sequencing in Jianchuan of Dali in Yunnan Province, aiming to provide data support for revealing the mechanism of continuous cropping obstacle of M. sextelata. The results indicated that the phylum Proteobacteria, genus Pseudomonas, species Bradyrhizo biumelkanii were dominant individually. Sphingomonas was the dominant genus in one-year soil, and Candidatus Jettenia was an additional genus, in which the species Streptomyces puniciscabiei significantly decreased, and Ignavibacteriales bacterium and Chlorobi bacterium were additional taxa, and Ignavibacteriales bacterium showed dominant in one-year soil. The OTUs and Alpha diversity analyses index in the original soil of M. sextelata were higher than those in one-year soil. The result revealed that the soil bacterial community structure changed significantly at the genus and species level, and the diversity and uniformity of species distribution in the community decreased after continuous cropping of M. sextelata. The analyses proposed that the continuous cropping obstacle of M. sextelata might be related to the decrease of relative abundance of various beneficial bacterial taxa in the soil, such as Acidobacteriota (phylum), Verrucomicrobiota (phylum), Pedosphaeraceae (family), Streptomyces puniciscabiei (species) and Candidatus Koribacter (genus).

Key words: Morchella sextelata, high-throughput sequencing, bacterial community, soil microorganisms, continuous cropping obstacles