The Response of the Main Functional Components of Red Raspberry Fruit and Leaves to Organic Fertilizer Application

doi:10.11924/j.issn.1000-6850.casb2022-0855

Abstract

Abstract:

The paper aims to investigate the effect of organic fertilizer on the content of the main functional components of red raspberry. The content of total ellagic acid, salicylic acid, total sugars, anthocyanin and polyphenols in the fruits and leaves were determined by applying base fertilizer of sheep manure and organic fungal fertilizer to double-season red raspberry ‘Heritage’ at different developmental phase (fertilizer application rates of 30×10³, 60×10³ and 120×10³ kg/hm², denoted by Y2, Y4, Y8, J2, J4, J8 respectively). Radar plot analysis was used to evaluate the fertilizer type and amount. The results showed that: (1) the total ellagic acid content of fruits and leaves reached the highest under J4 and J8 treatments at the second flowering and first fruiting stage, with 4.76 and 22.61 mg/g,respectively, which increased by 29.72% and 172.50% compared to CK. (2) The salicylic acid content of fruits and leaves were significantly higher than all other treatments under Y4 and Y2 at the second flowering period and first fruiting period, respectively, at 51.28 and 460.30 μg/g. (3) The total sugar content of fruits and leaves reached the highest under Y8 and Y2 treatments at the second fruiting period and first fruiting period, which were 10.10% and 41.38%. (4) The content of anthocyanin in fruits and leaves reached the highest level of 12.74 and 1.16 mg/g under J2 and Y2 treatments at the second flowering and early fruiting stages, respectively. (5) Fruits and leaves polyphenols contents reached the highest under Y4 and J4 in the second flowering period, which were 7.04 and 27.46 mg/g, respectively. The comprehensive evaluation of Radar chart showed that the highest comprehensive evaluation was achieved under Y2, J4, and J8 in the three resultant periods. The content of the main functional components in the fruits and leaves of red raspberry can be increased by the application of organic fertilizers. The J4 treatment was the best fertilization treatment, taking into account the actual cost requirements of production.

Key words: red raspberry, organic fertilizer, functional component, ellagic acid, salicylic acid

ZHANG Wei, CHANG Feiyang, ZHANG Peiyan, ZHANG Peifeng, ZHANG Xuemei. The Response of the Main Functional Components of Red Raspberry Fruit and Leaves to Organic Fertilizer Application[J]. Chinese Agricultural Science Bulletin, 2023, 39(27): 36-44.

Figures/Tables 7

References 42

[1]	国家药典委员会. 中华人民共和国药典:一部[S]. 北京: 中国医药科技出版社, 2015:399.
[2]	赵小曼, 李晔, 辛秀兰, 等. 红树莓活性成分提取物的应用研究进展[J]. 农产品加工, 2021(12):72-78.
[3]	房琳琳, 赵余庆. 树莓的现代药学及生物活性研究进展[J]. 沈阳药科大学学报, 2014, 31(1):74-80.
[4]	阴芳冉, 李颖, 于宏伟, 等. 不同产地红树莓果实营养成分分析及综合评价[J]. 河北农业大学学报, 2019, 42(4):48-52,66.
[5]	ANTTONEN M J, HOPPULA K I, NESTBY R, et al. Influence of fertilization, mulch color, early forcing, fruit order, planting date, shading, growing environment, and genotype on the contents of selected phenolics in strawberry (Fragaria × ananassa Duch.) fruits[J]. Journal of agricultural & food chemistry, 2006, 54(7):2614.
[6]	明广增, 徐建国, 刘向国. 有机肥施用量对‘映霜红’桃果品质及土壤养分的影响[J]. 北方果树, 2021(5):14-17.
[7]	查钱慧, 洪文泓, 谭莎, 等. 油茶饼粕有机肥对番茄生长表观及生理指标的影响[J]. 安徽农业大学学报, 2015, 42(3):458-462.
[8]	STOJANOV D, MILOŠEVIĆ T, MAŠKOVIĆ P, et al. Influence of organic, organo-mineral and mineral fertilisers on canetraits, productivity and berry quality of red raspberry (Rubus idaeus L.)[J]. Scientia horticulturae, 2019, 252:370-378. doi: 10.1016/j.scienta.2019.04.009 URL
[9]	杨苏, 刘耀斌, 王静, 等. 不同有机物料投入下黄河故道土壤有机碳积累特征的研究[J]. 土壤, 2021, 53(2):361-367.
[10]	温延臣, 张曰东, 袁亮, 等. 商品有机肥替代化肥对作物产量和土壤肥力的影响[J]. 中国农业科学, 2018, 51(11):2136-2142. doi: 10.3864/j.issn.0578-1752.2018.11.011
[11]	王宁, 南宏宇, 冯克云. 化肥减量配施有机肥对棉田土壤微生物生物量、酶活性和棉花产量的影响[J]. 应用生态学报, 2020, 31(1):173-181. doi: 10.13287/j.1001-9332.202001.022
[12]	张红梅, 金海军, 丁小涛, 等. 有机肥无机肥配施对温室黄瓜生长、产量和品质的影响[J]. 植物营养与肥料学报, 2014, 20(1):247-253.
[13]	田晓华. 有机肥施入量对树莓生长及产质量的影响[J]. 现代园艺, 2021, 44(13):12-13.
[14]	王光梅, 胡兵辉. 商品有机肥添加条件下间歇干旱对番茄生长生理及产量的影响[J]. 西南农业学报, 2021, 34(9):42-48
[15]	王迎, 齐国辉, 张雪梅, 等. 不同结果期红树莓果实中主要功能性成分含量变化[J]. 食品工业科技, 2017, 38(23):40-44.
[16]	马丛菲. 树莓果实中花色苷分析方法的研究[D]. 哈尔滨: 东北农业大学, 2018.
[17]	金茜, 赵特, 胡明华. 杨梅叶中花色苷提取工艺优化[J]. 中国食物与营养, 2018, 24(11):46-48,83.
[18]	王琬宁, 李兴国, 孙侨冶, 等. 红树莓非花色苷酚的提取与鉴定[J]. 北方园艺, 2020(10):22-31.
[19]	张文婷, 钮福祥, 孙健, 等. 不同品种甘薯叶片和块根中多酚含量的测定[J]. 现代农业科技, 2020(18):200-202.
[20]	波钦诺克H X. 植物生物化学分析方法[M]. 北京: 科学出版社,1981.
[21]	王俊刚, 张树珍, 杨本鹏, 等. 3,5-二硝基水杨酸(DNS)法测定甘蔗茎节总糖和还原糖含量[J]. 甘蔗糖业, 2008(5):45-49.
[22]	高文军, 李卫红, 王喜明, 等. 3,5-二硝基水杨酸法测定蔓菁中还原糖和总糖含量[J]. 中国药业, 2020, 29(9):113-116.
[23]	常世豪, 耿臻, 杨青春, 等. 改进雷达图分析法在大豆品种选育中的应用[J]. 大豆科学, 2020, 39(6):862-868.
[24]	安祥瑞, 江尚焘, 李焕苓, 等. 减施化肥配施有机肥对荔枝生长、产量品质及肥料利用率的影响[J]. 土壤, 2021, 53(6):1174-1184.
[25]	王瑛丽, 闫超超, 姜亚历, 等. 不同有机肥与无机肥配施对烤烟生长发育及产质量的影响[J]. 安徽农业科学, 2021, 49(22):161-163,169.
[26]	郑紫燕, 郑国琦, 罗霄, 等. 宁夏枸杞叶片、果柄、果实糖积累与蔗糖代谢相关酶活性研究[J]. 西北农业学报, 2009, 18(4):332-338.
[27]	李文云, 韩秀梅, 柏自琴, 等. UPLC-MS/MS分析牛肉红朱橘成熟果实多酚类化合物积累特征[J]. 食品工业科技, 2021, 42(22):11-17.
[28]	李树青. 不同欧李资源果实和叶片酚类物质含量及抗氧化活性的研究[D]. 呼和浩特: 内蒙古农业大学, 2021.
[29]	魏征. 圆叶葡萄多酚积累特性分析[D]. 北京: 中国农业大学, 2017:84-93.
[30]	孟雪娇, 邸昆, 丁国华. 水杨酸在植物体内的生理作用研究进展[J]. 中国农学通报, 2010, 26(15):217-224.
[31]	赵培臣, 贺殿. 逆境下拟南芥野生型和突变体sex1游离态水杨酸含量及根形态差异[J]. 西北植物学报, 2011, 31(12):2479-2485.
[32]	杨国慧, 范婷婷, 陈效杰, 等. 树莓叶果中水杨酸含量测定及其抑菌性分析[J]. 江苏农业科学, 2017, 45(23):181-183.
[33]	胡会刚, 杨转英, 庞振才, 等. 番石榴发育期生物活性物质研究[J]. 热带作物学报, 2018, 39(10):1940-1946.
[34]	赵慧芳, 吴文龙, 闾连飞, 等. 黑莓果实发育过程中多酚类物质的变化规律[J]. 吉林农业大学学报, 2013, 35(4):457-462.
[35]	ANTTONEN M J, KARJALAINEN R O. Environmental and genetic variation of phenolic compounds in red raspberry[J]. Journal of food composition & analysis, 2005, 18(8):759-769.
[36]	SAITO A. Changes in the polyphenol content of red raspberry fruits during ripening[J]. Horticulturae, 2021, 7(12):569. doi: 10.3390/horticulturae7120569 URL
[37]	孙乙铭, 许寿增, 俞春英, 等. 覆盆子成熟过程颜色表征及其与品质指标消长的相关性研究[J]. 中国中药杂志, 2021, 46(6):1379-1385.
[38]	王慧亮. 浙江栽培蓝莓品种果实品质分析及滤光膜和叶面肥对其影响[D]. 金华: 浙江师范大学, 2010:46-56.
[39]	甘金佳, 毛玲莉, 蒋水元, 等. 施用EM菌发酵有机肥对番茄植株生长·产量和品质的影响[J]. 安徽农业科学, 2021, 49(24):178-181.
[40]	韩明丽, 周坛根. 畜禽粪便与菌渣有机肥在白菜和黄瓜上的施用效果[J]. 浙江农业科学, 2021, 62(12):2442-2444.
[41]	马忠明, 陈娟, 牛小霞, 等. 减施化肥和配施有机肥对酿酒葡萄梅鹿辄产量和品质的影响[J]. 水土保持通报, 2021, 41(2):188-193,200.
[42]	文婷, 杨莉, 刘德春, 等. 遂川金柑果实品质与土壤、叶片、果实矿质养分的相关性分析[J]. 江西农业大学学报, 2021, 43(5):983-995.

处理	平均面积	平均周长	面积评价向量	评价值	排序
8-CK	0.95	4.12	0.71	0.82	5
8-Y2	1.88	5.48	0.79	1.21	1
8-Y4	1.01	4.24	0.71	0.85	3
8-Y8	0.90	4.06	0.69	0.79	6
8-J2	0.92	4.38	0.60	0.74	7
8-J4	1.00	4.23	0.70	0.84	4
8-J8	1.24	4.76	0.69	0.92	2
9-CK	1.15	4.17	0.83	0.98	4
9-Y2	1.02	4.38	0.67	0.83	7
9-Y4	1.12	4.16	0.81	0.96	5
9-Y8	1.07	4.12	0.79	0.92	6
9-J2	1.61	4.81	0.88	1.19	2
9-J4	1.92	5.44	0.81	1.25	1
9-J8	1.29	4.49	0.80	1.02	3
10-CK	1.39	4.84	0.75	1.02	5
10-Y2	1.65	4.81	0.89	1.21	4
10-Y4	1.08	4.03	0.84	0.95	7
10-Y8	1.05	3.89	0.87	0.95	6
10-J2	1.91	5.36	0.84	1.26	2
10-J4	1.80	5.08	0.88	1.26	3
10-J8	1.98	5.33	0.87	1.31	1

处理	平均面积	平均周长	面积评价向量	评价值	排序
8-CK	0.95	4.12	0.71	0.82	5
8-Y2	1.88	5.48	0.79	1.21	1
8-Y4	1.01	4.24	0.71	0.85	3
8-Y8	0.90	4.06	0.69	0.79	6
8-J2	0.92	4.38	0.60	0.74	7
8-J4	1.00	4.23	0.70	0.84	4
8-J8	1.24	4.76	0.69	0.92	2
9-CK	1.15	4.17	0.83	0.98	4
9-Y2	1.02	4.38	0.67	0.83	7
9-Y4	1.12	4.16	0.81	0.96	5
9-Y8	1.07	4.12	0.79	0.92	6
9-J2	1.61	4.81	0.88	1.19	2
9-J4	1.92	5.44	0.81	1.25	1
9-J8	1.29	4.49	0.80	1.02	3
10-CK	1.39	4.84	0.75	1.02	5
10-Y2	1.65	4.81	0.89	1.21	4
10-Y4	1.08	4.03	0.84	0.95	7
10-Y8	1.05	3.89	0.87	0.95	6
10-J2	1.91	5.36	0.84	1.26	2
10-J4	1.80	5.08	0.88	1.26	3
10-J8	1.98	5.33	0.87	1.31	1