Research Progress on the Extraction and Bioactivity of Flaxseed Lignan

doi:10.11924/j.issn.1000-6850.casb2023-0434

Abstract

Abstract:

Lignan is considered one of the most important bio-active components in flaxseed, and has attracted attention in recent years because of the efficacies of anti-cancer, antioxidation, anti-inflammation, diabetes prevention and cardiovascular system protection. In order to make a better understanding of the exploitation and utilization of flaxseed lignan, based on the domestic and foreign literature reviews, this article mainly reviewed the research progress on the extraction technology and biological activity application of flaxseed lignan. The effective extraction of lignan from flaxseed is the basis of bioactivity research. Different extraction processes can not only cause the difference in the extraction rate of lignan, but also affect its biological activity. This article summarizes some commonly used extraction methods for flaxseed lignan, including organic solvent extraction, subcritical water extraction and microbial fermentation. At the same time, the application of bioactivities is summed up and the research progress of lignan in the pharmaceutical field is emphasized. This article proposes prospects for the future research direction of flaxseed lignan, aiming to provide theoretical basis for the in-depth research, development and application of flaxseed lignan in the fields of medical industry and health industry.

Key words: Flaxseed lignan, extraction technology, extraction rate, bioactivity

JIANG Zhongjuan, YUAN Hongmei, SUN Yan. Research Progress on the Extraction and Bioactivity of Flaxseed Lignan[J]. Chinese Agricultural Science Bulletin, 2023, 39(32): 33-39.

Figures/Tables 2

References 64

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提取方法	提取条件	提取率	优点	缺点	文献来源
亚临界水提取法	料液比为1∶200，转速为 150 rpm，提取温度180℃，提取时间5 min。	27.13 mg/g	绿色环保，提取时间短，效率高	设备投资成本高，操作技术复杂	[29]
超声波辅助提取法	以水为溶剂，补充0.2 mol NaOH用于SDG-HMG复合物的碱性水解，在60℃和25 kHz超声频率下提取30 min。	24.07 mg/g	溶剂耗量少，省时高效	功率大，振动强，提取物中有多种物质溶出	[30]
微波辅助提取法	料液比1∶21.9，乙醇体积分数40.9%，微波功率130 W，提取时间90.5 s，提取温度<40℃。	21.91 mg/g	溶剂消耗量低，高效节能	辐射加热能量消耗大	[31]
有机溶剂提取法	料液比1∶24，乙醇体积分数68%，NaOH浓度0.10 mol/L，提取温度68℃，提取3 h。	20.31 mg/g	操作流程简单，设备要求低	溶剂消耗量大且提取时间长	[26]
微生物发酵提取法	采用米曲霉进行发酵，接菌量2.5%，发酵温度为22~25℃，发酵时间120 h。发酵后的亚麻籽粉加入50%丙酮，料液比1∶20，50℃水浴提取2 h。	16.89 mg/g	得率高，安全高效，节能环保	反应条件难以控制，提取时间长	[32]
超临界CO₂萃取法	乙醇浓度100%，提取压力9 MPa，提取温度35℃，搅拌转速50 r/min，提取时间30 min	11.80 mg/g	萃取效率高，污染小，环保	对设备及技术要求高，提取成本高	[33]
复合辅助提取法	果胶酶酶解后，料液比1∶6，乙醇体积分数71%，超声功率100 W，提取温度62℃，提取51 min。	11.59 mg/g	效率高，能量低，超声增强酶活性	提取参数难以控制，成本高	[34]
酶解法	复合酶（α-淀粉酶与纤维素酶），酶添加量1.4%，液料比 12∶1，酶解温度49℃，酶解液pH 4.4，酶解2 h。	8.27 mg/g	条件温和，能耗低，绿色环保	反应花费大且提取率较低	[35]

提取方法	提取条件	提取率	优点	缺点	文献来源
亚临界水提取法	料液比为1∶200，转速为 150 rpm，提取温度180℃，提取时间5 min。	27.13 mg/g	绿色环保，提取时间短，效率高	设备投资成本高，操作技术复杂	[29]
超声波辅助提取法	以水为溶剂，补充0.2 mol NaOH用于SDG-HMG复合物的碱性水解，在60℃和25 kHz超声频率下提取30 min。	24.07 mg/g	溶剂耗量少，省时高效	功率大，振动强，提取物中有多种物质溶出	[30]
微波辅助提取法	料液比1∶21.9，乙醇体积分数40.9%，微波功率130 W，提取时间90.5 s，提取温度<40℃。	21.91 mg/g	溶剂消耗量低，高效节能	辐射加热能量消耗大	[31]
有机溶剂提取法	料液比1∶24，乙醇体积分数68%，NaOH浓度0.10 mol/L，提取温度68℃，提取3 h。	20.31 mg/g	操作流程简单，设备要求低	溶剂消耗量大且提取时间长	[26]
微生物发酵提取法	采用米曲霉进行发酵，接菌量2.5%，发酵温度为22~25℃，发酵时间120 h。发酵后的亚麻籽粉加入50%丙酮，料液比1∶20，50℃水浴提取2 h。	16.89 mg/g	得率高，安全高效，节能环保	反应条件难以控制，提取时间长	[32]
超临界CO₂萃取法	乙醇浓度100%，提取压力9 MPa，提取温度35℃，搅拌转速50 r/min，提取时间30 min	11.80 mg/g	萃取效率高，污染小，环保	对设备及技术要求高，提取成本高	[33]
复合辅助提取法	果胶酶酶解后，料液比1∶6，乙醇体积分数71%，超声功率100 W，提取温度62℃，提取51 min。	11.59 mg/g	效率高，能量低，超声增强酶活性	提取参数难以控制，成本高	[34]
酶解法	复合酶（α-淀粉酶与纤维素酶），酶添加量1.4%，液料比 12∶1，酶解温度49℃，酶解液pH 4.4，酶解2 h。	8.27 mg/g	条件温和，能耗低，绿色环保	反应花费大且提取率较低	[35]