Detection and Survival Time of Erwinia amylovora in Mature Korla Fragrant Pear

doi:10.11924/j.issn.1000-6850.casb2023-0599

Abstract

Abstract:

To determine whether the Erwinia amylovora was on the mature pear and its survival time, mature fruits from different pear orchards in Korla region were collected. Through the quantitative detection method of living bacteria, the dynamic detection of E. amylovora on the stored pear was carried out, and the pathogenicity of the strains isolated from Zeller selective medium was determined. The survival time of E. amylovora in different parts of pear was as follows: surface (133 d)> calyx (83 d) / stalkhole (83 d)> peduncle (63 d). The viable cells of E. amylovora detection rates of different parts were as follows: fruit surface (6.88%)> calyx (3.75%)> stalkhole (2.19%)> peduncle (1.57%), and the isolated strains of E. amylovora had pathogenicity. In summary, strengthening the quarantine of Korla fragrant pear is an important method to prevent the disease from spreading across regions, and the results provide a theoretical basis for the risk assessment of pear as the vector of E. amylovora.

Key words: pear, fire blight, Erwinia amylovora, dynamic detection, survival time

ZHAO Zhiqiang, WEI Ran, GUO Qingyuan. Detection and Survival Time of Erwinia amylovora in Mature Korla Fragrant Pear[J]. Chinese Agricultural Science Bulletin, 2024, 40(19): 120-125.

Figures/Tables 5

References 26

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反应体系(25 μL)	qPCR反应程序
反应体系(25 μL)	预变性	变性	退火温度	延伸	循环数
TB Green^TMPremix Dimer Eraser 12.5 μL，浓度为10 μmol/L的上下游引物各0.75 μL， ROX为0.5 μL，模板2 μL，ddH₂O补足至25 μL	95℃，5 min	95℃，3 s	54℃，30 s	72，30 s	40

反应体系(25 μL)	qPCR反应程序
反应体系(25 μL)	预变性	变性	退火温度	延伸	循环数
TB Green^TMPremix Dimer Eraser 12.5 μL，浓度为10 μmol/L的上下游引物各0.75 μL， ROX为0.5 μL，模板2 μL，ddH₂O补足至25 μL	95℃，5 min	95℃，3 s	54℃，30 s	72，30 s	40

检测时间/d	检测数量/个	果柄				柄洼
检测时间/d	检测数量/个	阳性数量/ 个	检出率/ %	133 d内总检出率/ %	平均活菌数/ (CFU/样)	阳性数量/ 个	检出率/ %	133 d内总检出率/ %	平均活菌数/ (CFU/样)
3	40	1	2.5	1.57	7.0×10³	3	7.5	2.19	2.16×10⁴
23	40	0	-		-	0	-		-
43	40	0	-		-	0	-		-
63	40	4	10		8.45×10³	3	7.5		8.03×10³
83	40	0	-		-	1	2.5		9.54×10³
103	40	0	-		-	0	-		-
118	40	0	-		-	0	-		-
133	40	0	-		-	0	-		-
140	40	0	-	-	-	0	-	-	-
147	40	0	-	-	-	0	-	-	-
157	40	0	-	-	-	0	-	-	-
检测时间/d	检测数量/个	果萼				果面
检测时间/d	检测数量/个	阳性数量/ 个	检出率/ %	133 d内总检出率/ %	平均活菌数/ (CFU/样)	阳性数量/ 个	检出率/ %	133 d内总检出率/ %	平均活菌数/ (CFU/样)
3	40	8	20	3.75	1.49×10⁴	2	5	6.88	3.6×10⁴
23	40	1	2.5		8.67×10⁴	4	10		1.14×10⁴
43	40	1	2.5		2.4×10⁴	2	5		5.32×10⁴
63	40	0	-		-	2	5		6.17×10⁴
83	40	2	5		9.23×10³	5	12.5		1.11×10⁴
103	40	0	-		-	4	10		5.18×10³
118	40	0	-		-	1	2.5		1.1×10²
133	40	0	-		-	2	5		1.16×10²
140	40	0	-	-	-	0	-	-	-
147	40	0	-	-	-	0	-	-	-
157	40	0	-	-	-	0	-	-	-

检测时间/d	检测数量/个	果柄				柄洼
检测时间/d	检测数量/个	阳性数量/ 个	检出率/ %	133 d内总检出率/ %	平均活菌数/ (CFU/样)	阳性数量/ 个	检出率/ %	133 d内总检出率/ %	平均活菌数/ (CFU/样)
3	40	1	2.5	1.57	7.0×10³	3	7.5	2.19	2.16×10⁴
23	40	0	-		-	0	-		-
43	40	0	-		-	0	-		-
63	40	4	10		8.45×10³	3	7.5		8.03×10³
83	40	0	-		-	1	2.5		9.54×10³
103	40	0	-		-	0	-		-
118	40	0	-		-	0	-		-
133	40	0	-		-	0	-		-
140	40	0	-	-	-	0	-	-	-
147	40	0	-	-	-	0	-	-	-
157	40	0	-	-	-	0	-	-	-
检测时间/d	检测数量/个	果萼				果面
检测时间/d	检测数量/个	阳性数量/ 个	检出率/ %	133 d内总检出率/ %	平均活菌数/ (CFU/样)	阳性数量/ 个	检出率/ %	133 d内总检出率/ %	平均活菌数/ (CFU/样)
3	40	8	20	3.75	1.49×10⁴	2	5	6.88	3.6×10⁴
23	40	1	2.5		8.67×10⁴	4	10		1.14×10⁴
43	40	1	2.5		2.4×10⁴	2	5		5.32×10⁴
63	40	0	-		-	2	5		6.17×10⁴
83	40	2	5		9.23×10³	5	12.5		1.11×10⁴
103	40	0	-		-	4	10		5.18×10³
118	40	0	-		-	1	2.5		1.1×10²
133	40	0	-		-	2	5		1.16×10²
140	40	0	-	-	-	0	-	-	-
147	40	0	-	-	-	0	-	-	-
157	40	0	-	-	-	0	-	-	-

检测部位	阳性样品数量/个	检测结果/(CFU/样)
检测部位	阳性样品数量/个	PMA-qPCR	平板涂布计数
果柄	5	8.53×10³±1.33×10³	9.87×10³±2.39×10³
柄洼	7	1.41×10⁴±1.25×10⁴	1.63×10⁴±1.56×10⁴
果萼	12	1.42×10⁴±1.08×10⁴	1.64×10⁴±1.65×10⁴
果面	22	1.93×10⁴±1.18×10⁴	2.15×10⁴±2.38×10⁴