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Chinese Agricultural Science Bulletin ›› 2025, Vol. 41 ›› Issue (18): 90-98.doi: 10.11924/j.issn.1000-6850.casb2025-0019

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Effects of Substrate Ratio, Spike Type and Rooting Agent on Rooting of Styrax japonicas Cuttings

MO Fangshu1(), HE Jiamin2, LI Wenzhong3, CHEN Qin4, LIU Minhao2, XIAO Xingcui2()   

  1. 1 Xihua University, Chengdu 611743
    2 Sichuan Academy of Forestry/ Sichuan Yuchan Mountain Nanmu Cultivation National Longterm Scientific Research Base, Chengdu 610081
    3 Forestry Bureau of Yucheng District, Ya’an City, Ya’an, Sichuan 625099
    4 Xichang University, Xichang, Sichuan 615013
  • Received:2025-01-02 Revised:2025-04-17 Online:2025-06-25 Published:2025-07-06

Abstract:

To enhance rooting efficiency of Styrax japonicus cuttings, an orthogonal experimental design was conducted using current-season softwood and 1-year-old hardwood shoots. Five factors (substrate composition, cutting type, rooting hormone type, concentration, and immersion duration) were evaluated for their impacts on rooting performance. Results revealed a mixed rooting mechanism involving both callus and bark tissues, with root emergence occurring between 26-38 days post-cutting. Substrate type, cutting category, and hormone type demonstrated highly significant effects on rooting rate, normalized rooting rate, root count, and root length, while hormone concentration and immersion time significantly influenced root length. Optimization analysis prioritizing rooting rate identified the optimal conditions as: perlite: vermiculite (1:3) substrate, softwood cuttings, and treatment with 400 mg/L IBA solution for 60 minutes. Range analysis highlighted rooting hormone type as the most critical factor, followed by concentration, cutting type, substrate ratio, and immersion duration. Integrated findings recommended using a perlite: vermiculite (1:3) medium with softwood cuttings, pretreated in 400 mg/L IBA solution for 60 minutes, and conducted during spring for maximal propagation success.

Key words: Styrax japonicas, cuttage seedling, substrate ratio, cuttings type, rooting hormone type, rooting rate, survival rate