Abstract: Five mixed substrates formula: rockwool, perlite:peat=3:2, slag:peat=3:2, peanutshell:vermiculite:perlite= 5:3:2 and corncob:vermiculite:perlite=5:3:2 are conducted in tomato soilless culture. The results are as follows. Bulk density of five treatment increases by different degree during cultivation. The growth properties, photosynsythesis , chlorophyll content , root activity and yield of tomato have significant difference among different formula. Fresh mass, dry mass and leaf area of tomato plant cultivated in peanutshell:vermiculite:perlite=5:3:2 reach 705.24 g/plant, 73.871 g/plant, 7654.5cm2/plant respectively, which have significant difference compared with control’s. The early yield of five treatment are lower than control’s, of which the treatment of slag:peat=3:2 gets higher early yield and it reaches 23859.0 kg/hm2. The treatment of peanutshell:vermiculite:perlite=5:3:2 gets the highest total yield(78210.0 kg/hm2), increasing by 23.9 per cent compared with control’s yield. The tomato cultivated in slag:peat=3:2 gets the highest sugar/acid (7.58) and cultivated in peanutshell:vermiculite:perlite=5:3:2 gets the highest Vc content(0.1015 mg/g).