Abstract: Longnan is the nidus region of China’s wheat-infecting stripe rust (Puccinia striiformis West.).In order to achieve the goal of controlling of stripe rust in Longnan region, scientists have established the gene distribution technique for stripe rust control. But the effective methods on gene distribution have not been put forward particularly. Based on this problem, many researches have been conducted in Longnan area,which included the 40-year distributing character of wheat varieties, the situation of resistance resource used in practice and the yield trait of 42 varieties for 30 years. The result showed that the number of gene distribution is about 6 in Longnan region and that the varieties susceptible to stripe rust could be replaced with the resistant ones deriving from gene provision bank ,which is called “substitution method” in practice. This technique have great function on the implementation and improvement of gene distribution in Longnan area.