Genetic Performance of Dwarfing Gene Rht8 in Synthetic Hexaploid Wheat-Derived Populations

Abstract

Abstract: 【OBJECTIVE】 Synthetic hexaploid wheats obtained by crossing Aegilops tauschii Coss. (DD) with tetraploid wheats (AABB), containing genetic variability not found in hexaploid wheats, have been widely used for genetic improvement of common wheat. Understanding the distribution of Rht8 gene in synthetic hexaploid wheats × common wheat (Triticum aestivum.L) will be crucial for yield improvement. 【METHOD】 A total of 113 lines from BC2F2:6 three CIMMYT lines and four commercial cultivars by synthetic hexaploid wheats × common wheat crosses were used to detect Rht8 genotype by specific-primer PCR amplification and modified PAGE. 【RESULTS】 The frequency of Rht8 genotype is 77.78% in the detected derived-populations from syn768, syn769, syn780 and syn786 lines. But as for every population from syn768, syn769 and syn780 lines, it was concluded that the frequency is not the same. The frequency of Rht8 genotype of the population from syn768 is 96.70% as the highest one, and the one from syn769 with 71.64% is the lowest, and for the population from syn780, its frequency of Rht8 genotype is 73.68% with a close ratio of 3:1 and the cultivar Chuanmai 47 from syn786 is absent from the gene Rht8. 【CONCLUSION】.The inheritance of the dwarfing gene Rht8 is randomly separated, and the study on Rht8 genotype of progeny from crosses of synthetic hexaploid wheat and common wheat can provide useful information for improving common wheat and introducing cultivars from CIMMYT, and facilitating the analysis of Rht8 genotype polymorphism.

CLC Number:

S512

Yang Songjie,, Wang Yanjun, Li Jun, Liu Shigui, Yang Wuyun.. Genetic Performance of Dwarfing Gene Rht8 in Synthetic Hexaploid Wheat-Derived Populations[J]. Chinese Agricultural Science Bulletin, 2007, 23(2): 50-050.

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