Cloning and Preliminary Characterization of the BnZFP Gene from Brassica napus

Abstract

Abstract: To clone a new transcription factor from Brassica napus, and study its function. Semi-quantitative RT-PCR, Transformation, Northern-blotting. BnZFP was isolated from Brassica.napus, which constituted 377 amino acids. BnZFP had the highest similarity with AtZFP at nucleotide and amino acids level. Semi-quantitative RT-PCR showed BnZFP was steadily expressed throughout the different developmental stages between F1 and parental lines. BnZFP was constructed to pBI121 vector and this expression cassette was introduced into wild Arabidopsis thaliana. The transgenic plants were analysed by Northern blotting. Compared with wild Arabidopsis thaliana, over-expression of BnZFP in plants didn’t show any visible changes in seed germination and post-germination growth, further study should be carried out to study the gene’s function.

CLC Number:

Q786

Zhang Lan,, Wang Lei,, Tu Jinxing, Fan Yunliu,. Cloning and Preliminary Characterization of the BnZFP Gene from Brassica napus[J]. Chinese Agricultural Science Bulletin, 2007, 23(2): 45-045.

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