Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (7): 129-129.

Special Issue: 园艺

• 生物技术科学 • Previous Articles     Next Articles

Identification of Apple Varieties with SSR Molecular Markers

Caiqing, Jianglijie, Zhangxiaoming, Yanguohua, Zhangkaichun, Caoyufen, Mahuanpu   

  • Online:2007-07-05 Published:2007-07-05

Abstract: Levels of SSR-PCR factors were ascertained by orthogonal experiment in this paper(10×PCRBuffer2.0μl, 25mmol/MgCl21.2μl, 2mmol/LdNTPs2.0μl, 10μmol/L primer 1μl, 5U/μl Taq polymerase 0.2μl). Ten pairs of primers with high polymorphism and powerful distinctiveness have been selected from 33 pairs of primer. Three pairs of primer (02b1, GD12, GD162) could distinguish 45 cultivars used in the study. Application of SSR(simple sequence repeat) techniques for testing trueness and purity of apple varieties were discussed.

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