Sequence Analysis of Canine Parvovirus VP2 Gene

Abstract

Abstract: Using polymerase chain reaction (PCR), we amplified the complete VP2 gene of twenty canine parvovirus samples collected in small animal hospital of China Agricultural University from 2006 to 2007. sequence analysis of nucleotide and deduced amino acid residues revealed that nineteen samples were CPV-2a and one CPV-2b among the twenty canine parvovirus. All samples had an amino acid substitution of residue 297 in VP2, Ser to Ala. nineteen CPV-2a samples studied in this study had reversion substitution (Ile→Val) at residue 555 in VP2. Compared with classical CPV-2a/b isolates, some samples had new amino acid substitutions: Samples CPV/BJ005/07 and CPV/BJ008/07 at amino acid residue 139 in VP2 was Ile instead of Val; Ile-324 variants accounted to 80% (16/20). Amino acid substitutions of residue 226 (Ser→Asn) and 382 (Arg→Lys)were found in samples CPV/BJ004/07and CPV/BJ050/07, respectively. All (Ile-139, Ile-324, Asn-226, and Lys-382) except Ile-139 substitutions were all in regions of potential antigen epitopes by DNAStar software analysis, and these substitutions may have an important influence to viral biology aspect.

CLC Number:

Song Yongqi, Li Yuyan, Lu Yanli, Han Bo. Sequence Analysis of Canine Parvovirus VP2 Gene[J]. Chinese Agricultural Science Bulletin, 2008, 24(7): 26-31.

References

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