Effect of Volatile Oil from Chenopodium ambrosioides on SOD and POD Isozymes in Root Tip of Vicia faba

doi:10.11924/j.issn.1000-6850.casb15050154

Abstract

Abstract: This study aimed to understand the effect of volatile oil from Chenopodium ambrosioides L. on antioxidant system of receptor plants, and provide theoretical basis for clarifying the allelopathic mechanism of C. ambrosioides. Through simulating eluviation and volatilization, the effects of volatile oil from C. ambrosioides (treatment doses were 5, 10, 15, 20 μL and treatment time periods were 24, 48, 72 h respectively) on the characteristics of isozyme bands of supemxide dismutase (SOD) and peroxidase (POD) in root tip cells of Vicia faba were compared by electrophoresis. The results showed that in the eluviation, the cell SOD isozyme activities were significantly inhibited by the volatile oil treatment, and a characteristic strong band of POD7 was observed after 48 h treatment. In the volatilization, the SOD isozyme activities were enhanced after the volatile oil treatment, a new characteristic band of POD6 was expressed in higher dose and longer time treatment of the volatile oil (24 h, 20 μL; 48 h, 15-20 μL; 72 h, 10-20 μL). Moreover, SOD isozyme bands showed mainly the changes of band intensity, but POD isozyme bands showed mainly the changes of band number. It was speculated that C. ambrosioides volatile oil may affect the gene expression of SOD and POD isozymes in receptor broad bean root tip cells, of which the responses of POD isozymes could be more sensitive than that of SOD isozymes.

CLC Number:

S181

胡琬君,, and . Effect of Volatile Oil from Chenopodium ambrosioides on SOD and POD Isozymes in Root Tip of Vicia faba[J]. Chinese Agricultural Science Bulletin, 2015, 31(29): 47-53.

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