Screening, Identification and Antagonism Evaluation of Antagonistic Bacteria Against Soil-borne Diseases of Tomato

doi:10.11924/j.issn.1000-6850.casb18090042

Abstract

Abstract:

Soil-borne diseases seriously affect the yield and economic benefits of tomato, and it is of great significance to select excellent antagonistic microorganism for biological control of soil-borne diseases. In this study, NSB-26 and RSB-6 two antagonistic strains were isolated from rhizosphere soil of tomato using four pathogenic fungi (Rhizoctonia solani, Fusarium oxysporum, Fusarium oxysporum and Alternaria) of tomato root rot. NSB-26 and RSB-6 were identified as Bacillus velezensis and Bacillus siamensis by physiological and biochemical tests and 16S rDNA analysis, respectively. The pot experiment was carried out to verify the effect of the obtained strain on tomato root rot disease. Three treatments were set: treated with pathogen (T1), treated with antagonistic bacteria and pathogen mixture (T2), and treated with equal amount of water (CK). The results showed that the plant height, root length, the dry and fresh weight of T2 significantly increased compared with those of T1 and CK. The maximum increase range of plant height in T1 and T2 was 10.06% and 3.95%, respectively; and the increase of root length was up to 67.09% and 26.48%, respectively. After 15 days treatment, the maximum increase range of fresh weight of T1 and T2 was 133.21% and 93.20%, respectively, and that of dry weight was 126.42% and 81.82%, respectively. In addition, chlorophyll a and b content also increased significantly. In conclusion, antagonistic treatment could effectively reduce the soil borne disease and increase the resistance of tomato seedling, providing a research basis for further development and utilization of tomato rhizosphere antagonistic bacteria.

Key words: tomato, soil-borne disease, antagonistic bacteria, rhizosphere soil, screening, identification, evaluation

CLC Number:

S436.412

Chi Xiaoli, Liu Kexin, Xu Chao, Liu Weiwei, Zhang Xiao, Zhu Hao, Jin Xiao, Sun Zhongtao, Liu Liying. Screening, Identification and Antagonism Evaluation of Antagonistic Bacteria Against Soil-borne Diseases of Tomato[J]. Chinese Agricultural Science Bulletin, 2020, 36(3): 135-141.

Figures/Tables 7

References 33

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菌株	立枯丝核菌	尖孢镰刀菌	腐皮镰刀菌	链格孢菌
NSB-26	57.6	78.0	53.0	56.0
RSB-6	57.4	73.0	56.8	62.5

菌株	立枯丝核菌	尖孢镰刀菌	腐皮镰刀菌	链格孢菌
NSB-26	57.6	78.0	53.0	56.0
RSB-6	57.4	73.0	56.8	62.5

		标准菌株	自筛菌	标准菌株	自筛菌
菌株编号		Bacillus velezensis	NSB-26	Bacillus siamensis	RSB-6
硝酸盐还原		+	+	+	+
D-葡萄糖产酸		+	+	+	+
L-阿拉伯产酸		+	+	+	+
D-木糖产酸		+	+	+	+
蔗糖产酸		+	+	+	+
葡萄糖产气		+	+	-	+
耐盐性	5% NaCl	+	+	+	+
	7% NaCl	+	+	+	+
	10% NaCl	+	+	+	+
反硝化试验		-	-	+	+
接触酶		+	-	-	-
甲基红		+	+	+	+
运动性		+	+	+	+
吲哚		-	-	-	-
V-P		-	+	+	+
明胶液化		+	+	+	+
淀粉水解		+	+	+	+
柠檬酸盐利用		-	+	+	+
丙二酸盐利用		+	+	+	+

		标准菌株	自筛菌	标准菌株	自筛菌
菌株编号		Bacillus velezensis	NSB-26	Bacillus siamensis	RSB-6
硝酸盐还原		+	+	+	+
D-葡萄糖产酸		+	+	+	+
L-阿拉伯产酸		+	+	+	+
D-木糖产酸		+	+	+	+
蔗糖产酸		+	+	+	+
葡萄糖产气		+	+	-	+
耐盐性	5% NaCl	+	+	+	+
	7% NaCl	+	+	+	+
	10% NaCl	+	+	+	+
反硝化试验		-	-	+	+
接触酶		+	-	-	-
甲基红		+	+	+	+
运动性		+	+	+	+
吲哚		-	-	-	-
V-P		-	+	+	+
明胶液化		+	+	+	+
淀粉水解		+	+	+	+
柠檬酸盐利用		-	+	+	+
丙二酸盐利用		+	+	+	+