Sambucus williamsii Clones at Seedling Stage: Response to Drought Stress and Comprehensive Evaluation of Drought Resistance

doi:10.11924/j.issn.1000-6850.casb18120009

Abstract

Abstract:

The paper aims to reveal the drought resistance of different clones of Sambucus williamsii, to explore the adaptability of Sambucus williamsii to arid environment, and to provide experimental materials for afforestation in arid and barren mountains. We carried out the natural drought stress test by pot experiment method, and comprehensively evaluated the physiological indexes of each clone under different treatments by using the principal component analysis. The results showed that: the biomass increment of PT-1 was the largest, the root-shoot ratio of PT-2 was the largest under drought stress; with the increase of drought stress, the permeability of cell membrane increased, the permeability cell membrane of PT-2 and PT-1 was the smallest when the drought reached moderate and severe degree; the chlorophyll content and the SOD activity increased firstly and then decreased, the soluble sugar and proline increased with the increase of drought stress; under severe drought stress, the soluble sugar content of PT-1 was the highest. The principal component analysis showed that: the drought resistance ability from strong to weak was XY-2, JY-1, XY-1, XY-3, PT-1, PT-3, PT-2, among them, XY-2 and JY-1 had relatively strong drought resistance and could be further studied as drought-resistant clonal materials.

Key words: Sambucus williamsii, clone, seedling stage, drought resistance, physiological index, comprehensive evaluation

CLC Number:

S326

Junxiu Yao, Xueliang Liu, Shanwen Li, Fei Ren, Qinghua Li, Haitao Wu, Honglian Zhai, Dejun Wu. Sambucus williamsii Clones at Seedling Stage: Response to Drought Stress and Comprehensive Evaluation of Drought Resistance[J]. Chinese Agricultural Science Bulletin, 2020, 36(9): 75-81.

Figures/Tables 11

References 23

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序号	简称	无性系	来源
1	JY-1	金叶接骨木优株	山东省林科院中试基地
2	XY-1	西洋接骨木优株	济南平阴县孔村
3	PT-1	接骨木优株	济南平阴县孔村
4	PT-2	接骨木优株	山东博华高效生态农业科技有限公司
5	PT-3	接骨木优株	江苏丰县
6	XY-2	西洋接骨木优株	山东省林科院中试基地
7	XY-3	西洋接骨木优株	山东省林科院中试基地

序号	简称	无性系	来源
1	JY-1	金叶接骨木优株	山东省林科院中试基地
2	XY-1	西洋接骨木优株	济南平阴县孔村
3	PT-1	接骨木优株	济南平阴县孔村
4	PT-2	接骨木优株	山东博华高效生态农业科技有限公司
5	PT-3	接骨木优株	江苏丰县
6	XY-2	西洋接骨木优株	山东省林科院中试基地
7	XY-3	西洋接骨木优株	山东省林科院中试基地

无性系	土壤相对含水量/%
无性系	无干旱(0 d)	轻度干旱(5 d)	中度干旱(10 d)	重度干旱(15 d)
JY-1	102.3	65.8	42.4	27.9
XY-1	101.2	64.2	43.5	21.3
PT-1	103.5	63.9	42.2	27.5
PT-2	100.4	63.1	39.3	25.9
PT-3	102.8	64.5	39.4	25.8
XY-2	102.4	67.2	40.5	30.4
XY-3	100.1	65.3	40.8	25.4

无性系	土壤相对含水量/%
无性系	无干旱(0 d)	轻度干旱(5 d)	中度干旱(10 d)	重度干旱(15 d)
JY-1	102.3	65.8	42.4	27.9
XY-1	101.2	64.2	43.5	21.3
PT-1	103.5	63.9	42.2	27.5
PT-2	100.4	63.1	39.3	25.9
PT-3	102.8	64.5	39.4	25.8
XY-2	102.4	67.2	40.5	30.4
XY-3	100.1	65.3	40.8	25.4

测定指标	中度干旱			重度干旱
测定指标	Prin1	Prin2	Prin3	Prin1	Prin2	Prin3
特征根	5.36	1.46	1.02	4.76	1.60	1.49
累计贡献率/%	59.56	75.80	87.09	52.94	70.66	87.28
苗高增量(X1)	0.89	0.06	0.09	0.78	-0.30	0.45
地径增量(X2)	0.15	0.89	-0.22	-0.02	0.81	-0.11
根冠比(X3)	-0.84	-0.27	-0.08	-0.97	0.00	-0.04
生物量增量(X4)	-0.81	-0.38	-0.33	-0.78	-0.35	-0.46
细胞膜透性(X5)	0.35	0.65	0.62	0.94	-0.03	0.23
叶绿素(X6)	0.13	-0.09	0.95	0.12	0.02	0.94
超氧化物岐化酶SOD(X7)	0.35	0.86	0.23	0.73	0.35	-0.39
可溶性糖(X8)	-0.76	-0.19	-0.46	-0.19	-0.75	-0.54
脯氨酸(X9)	0.77	0.51	0.00	0.78	0.51	0.04