Low Temperature Stress at Soybean Flowering Stage: Effect on Sucrose Metabolism of Leaves and Yield

doi:10.11924/j.issn.1000-6850.casb2020-0240

Abstract

Abstract:

To study the effect of low temperature stress on the yield formation of soybean at flowering stage, ‘Kenfeng 16’ (chilling tolerant variety) and ‘Hefeng 50’ (chilling sensitive variety) were used as research materials, the sucrose content and metabolic enzyme activities were determined with pot experiment. The yield was measured at maturity stage. The results showed that plant height and yield were significantly reduced in both soybean varieties, and the height of bottom pods was increased. With the low temperature treatment prolonging, the production reduction was gradually increased. Compared with the control (H-CK and K-CK), the yield of ‘Hefeng 50’ was significantly decreased by 10.29%-30.88%, and that of ‘Kenfeng 16’ was significantly decreased by 9.58%-24.22%. The production reduction of chilling sensitive variety was higher than that of chilling tolerant variety. Starch and sucrose content declined and fructose content increased in leaves with the low temperature treatment prolonging. The activities of acid invertase, neutral invertase and sucrose synthase were improved, and the activity of sucrose phosphate synthase was inhibited by low temperature stress. The above indicators showed opposite trends in both soybean varieties during recovery period (1-4 days in natural environment after 4 days of low temperature treatment). The catabolism pathway of sucrose was accelerated, and the synthesis pathway of sucrose was inhibited, and finally sucrose content in leaves was drastically reduced when soybeans were exposed to low temperature stress during the flowering period, and the damage could not be restored within 4 days under natural environment. This is not conducive to the formation of soybean yield, and could be regarded as one of the important factors of soybean yield reduction caused by low temperature stress.

Key words: soybean (Glycine max L.), low temperature stress, flowering stage, sucrose metabolism, yield

CLC Number:

S859.84

Zhao Jingjing, Zhou Nong, Zheng Dianfeng. Low Temperature Stress at Soybean Flowering Stage: Effect on Sucrose Metabolism of Leaves and Yield[J]. Chinese Agricultural Science Bulletin, 2021, 37(9): 1-8.

Figures/Tables 6

References 25

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品种	处理	产量/g	单株荚数/个	单株粒数/个
合丰50	CK	9.91±0.76 a	30.50±2.31 b	77.50±5.30 a
	C1	8.89±0.89 ab	24.44±1.25 c	47.18±3.58 c
	C2	7.76±0.63 b	31.00±1.85 a	51.13±4.23 b
	C3	7.20±0.76 b	28.89±1.09 b	51.86±2.22 b
	C4	6.85±0.33 c	22.40±2.09 c	50.80±3.41 b
垦丰16	CK	9.29±0.54 a	24.63±2.11 a	90.40±7.14 a
	C1	8.40±0.38 b	19.86±0.97 b	82.13±6.55 b
	C2	7.61±0.60 c	19.50±1.59 b	80.90±7.15 bc
	C3	7.19±0.57 c	18.55±1.89 b	61.33±5.23 c
	C4	7.04±0.35 c	21.00±2.07 ab	58.40±3.52 c

品种	处理	产量/g	单株荚数/个	单株粒数/个
合丰50	CK	9.91±0.76 a	30.50±2.31 b	77.50±5.30 a
	C1	8.89±0.89 ab	24.44±1.25 c	47.18±3.58 c
	C2	7.76±0.63 b	31.00±1.85 a	51.13±4.23 b
	C3	7.20±0.76 b	28.89±1.09 b	51.86±2.22 b
	C4	6.85±0.33 c	22.40±2.09 c	50.80±3.41 b
垦丰16	CK	9.29±0.54 a	24.63±2.11 a	90.40±7.14 a
	C1	8.40±0.38 b	19.86±0.97 b	82.13±6.55 b
	C2	7.61±0.60 c	19.50±1.59 b	80.90±7.15 bc
	C3	7.19±0.57 c	18.55±1.89 b	61.33±5.23 c
	C4	7.04±0.35 c	21.00±2.07 ab	58.40±3.52 c

品种	处理	株高/cm	茎粗/mm	底荚高度/cm
合丰50	CK	62.01±3.23 a	6.09±0.23 a	11.93±1.36 b
	C1	60.89±2.11 b	5.66±0.42 a	11.36±1.76 b
	C2	60.75±2.04 b	5.54±0.11 a	13.89±0.91 a
	C3	60.66±0.93 b	5.85±0.31 a	13.96±2.00 a
	C4	57.64±1.03 c	5.26±0.11 a	14.28±1.75 a
肯丰16	CK	63.40±3.00 a	6.16±0.09 a	13.98±1.37 b
	C1	61.50±2.34 b	5.19±0.44 a	12.49±0.93 b
	C2	61.07±1.04 b	5.43±0.24 a	14.55±0.88 a
	C3	58.34±2.01 c	5.31±0.32 a	14.13±1.04 a
	C4	58.00±3.12 c	5.24±0.40 a	15.38±2.00 a

品种	处理	株高/cm	茎粗/mm	底荚高度/cm
合丰50	CK	62.01±3.23 a	6.09±0.23 a	11.93±1.36 b
	C1	60.89±2.11 b	5.66±0.42 a	11.36±1.76 b
	C2	60.75±2.04 b	5.54±0.11 a	13.89±0.91 a
	C3	60.66±0.93 b	5.85±0.31 a	13.96±2.00 a
	C4	57.64±1.03 c	5.26±0.11 a	14.28±1.75 a
肯丰16	CK	63.40±3.00 a	6.16±0.09 a	13.98±1.37 b
	C1	61.50±2.34 b	5.19±0.44 a	12.49±0.93 b
	C2	61.07±1.04 b	5.43±0.24 a	14.55±0.88 a
	C3	58.34±2.01 c	5.31±0.32 a	14.13±1.04 a
	C4	58.00±3.12 c	5.24±0.40 a	15.38±2.00 a