Changes of Key Enzyme Activities for Salvianolic Acid Biosynthesis Before and After Flowering in Salvia miltiorrhiza Polyploid

doi:10.11924/j.issn.1000-6850.casb2021-0901

Abstract

Abstract:

To study the effect of flowering on salvianolic acid accumulation, the changes of the key enzymes activities for salvianolic acid biosynthesis were studied at bud, flowering and decaying stage in Salvia miltiorrhiza polyploid. The results showed that soluble protein increased first and then decreased, and reached the peak at flowering stage of S. miltriorrhiza polyploid, and the soluble protein content in No.5 S. miltriorrhiza leaves was higher than that of other S. miltriorrhiza polyploid at all stages. Tyrosin aminotransferase (TAT) enzyme activity at bud stage was higher than that after flowering, especially low at full flowering stage. The activity of phenylalanine ammonialyase (PAL) enzyme increased gradually in different S. miltriorrhiza polyploid. The change of cinnamic acid 4-hydroxylase (C4H) enzyme activity firstly decreased and then increased during the flowering stage. However, the TAT, PAL and C4H enzyme activities in No.5 S. miltriorrhiza were higher than those of other S. miltriorrhiza polyploid at all stages, which might be one of the reasons why salvianolic acid content in No.5 S. miltriorrhiza was higher than that of others.

Key words: Salvia miltiorrhiza polyploid, flowering, salvianolic acid, tyrosin aminotransferase, cinnamic acid 4-hydroxylase, phenylalanine ammonialyase

CLC Number:

WU Shun, MA Xiaojing, QIU Yun, SUN Jianpeng, ZHAI Xi, LIU Qi. Changes of Key Enzyme Activities for Salvianolic Acid Biosynthesis Before and After Flowering in Salvia miltiorrhiza Polyploid[J]. Chinese Agricultural Science Bulletin, 2022, 38(19): 73-76.

Figures/Tables 4

References 26

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