Effects of Pennisetum giganteum on Growth, Slaughter Performance and Gene Expression of H-FABP and A-FABP in Phasianus colchicus

doi:10.11924/j.issn.1000-6850.casb2022-0011

Abstract

Abstract:

The purpose of this experiment is to investigate the effect of adding Pennisetum giganteum in diet on growth indexes, slaughter performance, and fat-related genes of heart-type fatty acid-binding protein (H-FABP) and adipocyte fatty acid-binding protein (A-FABP) gene in Phasianus colchicus. The experiment selected 150 healthy P. colchicus (4 weeks old) in the same batch and randomly divided them into 4 experimental groups and 1 control group, 10 in each group, 3 replicates. The addition amount for the experimental groups was 4%, 8%, 12%, and 16%, respectively. The control group was fed with basal diet, and the feeding period was 16 weeks. Compared with that of the control group, the difference of the average body weight of the 8% P. giganteum adding group was significantly increased (P<0.05); the average daily feed intake increased, but the difference was not significant (P>0.05); the average daily weight increase of each experiment group was significantly greater than that of the control group (P<0.05), among which the 8% P. giganteum adding group had the most significant improvement effect. The 4%, 8%, and 12% P. giganteum adding groups had a decrease in the ratio of feed to weight; and compared with those of the control group, the slaughter rate, half evisceration rate and complete evisceration rate of the 8% P. giganteum adding group significantly increased (P<0.05). The breast muscle rate of P. colchicus in the P. giganteum adding groups increased, but the difference was not significant compared with that of the control group (P>0.05); the leg muscle rate in the 8% P. giganteum adding group was significantly higher than that in the control group (P<0.05). The differential expression of H-FABP and A-FABP genes were determined by real-time fluorescence quantitative PCR after the addition of P. giganteum diet, and were significantly increased in the 8% P. giganteum adding group. Therefore, adding 8% of P. giganteum to the diet can significantly improve the growth index, slaughter performance and the expression of fat-related genes in P. colchicus.

Key words: Phasianus colchicus, Pennisetum giganteum, growth performance, slaughter performance, H-FABP gene, A-FABP gene

CLC Number:

S828.5

HAO Liuliu, DENG Xinwei, QIU Bowen, SUN Zhihong. Effects of Pennisetum giganteum on Growth, Slaughter Performance and Gene Expression of H-FABP and A-FABP in Phasianus colchicus[J]. Chinese Agricultural Science Bulletin, 2022, 38(35): 105-110.

Figures/Tables 7

References 34

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检测项目	检测方法	检测结果
粗灰分	GB/T 6438—2007	19.4%
粗蛋白质	GB/T 6432—1994	27.2%
粗脂肪	GB/T 6433—2006	2.2%
粗纤维	GB/T 6434—2006	16%
钙	GB/T 6436—2002	6.74%
磷	GB/T 6437—2002	4.95%

检测项目	检测方法	检测结果
粗灰分	GB/T 6438—2007	19.4%
粗蛋白质	GB/T 6432—1994	27.2%
粗脂肪	GB/T 6433—2006	2.2%
粗纤维	GB/T 6434—2006	16%
钙	GB/T 6436—2002	6.74%
磷	GB/T 6437—2002	4.95%

项目		巨菌草添加水平/%
项目		0	4	8	12	16
日粮配方/ %	玉米	31.0	31.0	31.0	31.0	31.0
	豆粕	27.0	28.50	30.0	31.5	33.0
	麸皮	16.0	12.0	8.0	4.0	0
	鱼粉	5.0	5.0	5.0	5.0	5.0
	骨粉	1.0	1.0	1.0	1.0	1.0
	预混料	19.0	17.50	16.0	14.50	13.0
	维生素	0.2	0.2	0.2	0.2	0.2
	食盐	0.3	0.3	0.3	0.3	0.3
	磷酸钙	0.5	0.5	0.5	0.5	0.5
	巨菌草	0	4.0	8.0	12.0	16.0
合计		100.0	100.0	100.0	100.0	100.0
营养水平	消化能/(MJ/kg)	6.28	5.39	6.55	5.46	6.09
	粗蛋白质/%	22.16	22.37	22.58	22.79	22.99
	粗纤维/%	15.08	14.01	12.94	11.87	10.80

项目		巨菌草添加水平/%
项目		0	4	8	12	16
日粮配方/ %	玉米	31.0	31.0	31.0	31.0	31.0
	豆粕	27.0	28.50	30.0	31.5	33.0
	麸皮	16.0	12.0	8.0	4.0	0
	鱼粉	5.0	5.0	5.0	5.0	5.0
	骨粉	1.0	1.0	1.0	1.0	1.0
	预混料	19.0	17.50	16.0	14.50	13.0
	维生素	0.2	0.2	0.2	0.2	0.2
	食盐	0.3	0.3	0.3	0.3	0.3
	磷酸钙	0.5	0.5	0.5	0.5	0.5
	巨菌草	0	4.0	8.0	12.0	16.0
合计		100.0	100.0	100.0	100.0	100.0
营养水平	消化能/(MJ/kg)	6.28	5.39	6.55	5.46	6.09
	粗蛋白质/%	22.16	22.37	22.58	22.79	22.99
	粗纤维/%	15.08	14.01	12.94	11.87	10.80

基因名称	引物序列	片段长度
β-action-F	GAGAAATTGTGCGTGACATCA	620
β-action-R	CCTGAACCTCTCATTGCCA	620
A-FABP-F	TGAGACCACAGCAGATGACAG	1038
A-FABP-R	GGTTCCCATCCACCACTTT	1038
H-FABP-F	ATGGTGGAAGCGTTCGTG	987
H-FABP-R	TGATGGTGGTGGGTTTGG	987