Response of Brassica napus Varieties at Seedling Stage to Different Waterlogging Environments

doi:10.11924/j.issn.1000-6850.casb2023-0553

Abstract

Abstract:

The paper aims to screen out the varieties of Brassica napus with waterlogging tolerance, to provide a basis for high-quality and moisture-resistant rapeseed varieties in Sichuan and other rice-oil rotation mode areas. In this experiment, the B. napus varieties ‘Chuanyou 36’, ‘Chuanyou 46’, ‘Chuanyou 83’, ‘Chuanzaoyou 1’, ‘Yuyou 35’and ‘Chunxi 1402’, which were mainly promoted in Sichuan, Chongqing and Yunnan, were used as experimental materials. Indoor water flooding culture was carried out for 3, 5 and 7 days to simulate the field environment under different degrees of waterlogging stress, to study the response of B. napus varieties to different wet damage environments at seedling stage. It was found that the comprehensive wet-damage index was positively correlated with germination rate and fresh weight of seedlings, but not with the length of main roots and plant height. The waterlogging tolerance of various B. napus varieties at seedling stage was ‘Chuanyou 83’> ‘Yuyou 35’> ‘Chunxi 1402’> ‘Chuanyou 36’>‘Chuanzaoyou 1’> ‘Chuanyou 46’. To sum up, the B. napus with waterlogging tolerance at seedling stage can be determined by germination rate and fresh weight of seedlings, and the comprehensive wet-damage index can be directly used to evaluate its waterlogging tolerance. ‘Chuanyou 83’, a B. napus variety with strong waterlogging tolerance, was selected at seedling stage in this experiment.

Key words: Brassica napus, waterlogging resistance, variety screening, comprehensive wet-damage index

YE Wenwei, MI Luo, LI Feixia, LIU Ranjin, DENG Chaoyang, LI Chaosu, LI Ming, ZHUANG Juan, ZHAO Wei, LIAO Yingxiu, WANG Zhihua, JING Shuzhong, CHENG Bin. Response of Brassica napus Varieties at Seedling Stage to Different Waterlogging Environments[J]. Chinese Agricultural Science Bulletin, 2024, 40(16): 21-25.

Figures/Tables 3

References 26

[1]	CHENG H, HAO M, WANG W, et al. Integrative RNA- and miRNA-profile analysis reveals a likely role of BR and auxin signaling in branch angle regulation of B. napus[J]. International journal of molecular sciences, 2017, 18(5):887.
[2]	郭燕枝, 杨雅伦, 孙君茂. 我国油菜产业发展的现状及对策[J]. 农业经济, 2016(7):44-46.
[3]	陈雅慧. 油菜萌发期耐淹性种质资源筛选与遗传机制解析[D]. 武汉: 华中农业大学, 2019.
[4]	张菡, 郑昊, 王明田, 等. 基于GIS的秋季连阴雨对农业生产影响的风险评估——以四川省盆地区为例[J]. 应用生态学报, 2017, 28(8):2569-2576.
[5]	伊淑丽, 徐洪志, 廖淑梅, 等. 油菜湿害及其防治措施研究进展[J]. 南方农业, 2012, 6(1):83-85.
[6]	陈娟妮, 梁颖. 长江流域主要甘蓝型油菜品种苗期耐湿性鉴定[J]. 中国生态农业学报, 2011, 19(3):626-630.
[7]	XU M, MA H, ZENG L, et al. The effect of waterlogging on yield and seed quality at the early flowering stage in Brassica napus L.[J]. Field crops research, 2015, 180:238-245.
[8]	李翠金. 中国暴雨洪涝灾害的统计分析[J]. 灾害学, 1996(1):59-63.
[9]	李浩杰, 柴靓, 蒲晓斌, 等. 室内水淹和田间模拟湿害对甘蓝型油菜耐湿性鉴定[J]. 西南农业学报, 2016, 29(6):1250-1256.
[10]	李云. 甘蓝型油菜苗期耐淹性鉴定和不同耐淹性材料的光合参数差异[D]. 南京: 南京农业大学, 2011.
[11]	刘凯文, 苏荣瑞, 潘建成, 等. 春季湿渍害对油菜产量结构的影响与减产效应[J]. 江苏农业科学, 2018, 46(5):63-66.
[12]	唐章林, 王霖, 张娅茹, 等. 甘蓝型油菜种质资源苗期耐湿性综合评价与筛选[J]. 西南大学学报(自然科学版), 2022, 44(12):19-28.
[13]	文艺. 浅谈油菜高产栽培技术[J]. 农业开发与装备, 2016(3):125.
[14]	ZOU X, ZENG L, LU G, et al. Comparison of transcriptomes undergoing waterlogging at the seedling stage between tolerant and sensitive varieties of Brassica napus L.[J]. Journal of integrative agriculture, 2015, 14(9):1723-1734.
[15]	徐明月. 甘蓝型油菜发芽期耐渍相关基因的筛选[D]. 北京: 中国农业科学院, 2014.
[16]	尹继春, 张燕, 万子良. 油菜耐湿性鉴定初报[J]. 上海农业科技, 1980(6):5-7.
[17]	卢长明, 龚学明. 缺氧条件下油菜种子发芽特性的遗传参数研究[J]. 江西农业大学学报, 1989(4):23-26.
[18]	陈洁. 甘蓝型油菜对湿害胁迫的适应机理研究[D]. 重庆: 西南大学, 2007.
[19]	张雪花, 李浩杰, 张锦芳, 等. 甘蓝型油菜耐湿性初步评价和主成分分析[J]. 中国油料作物学报, 2011, 33(2):98-103.
[20]	阳小虎. 温度对甘蓝型油菜和白菜型油菜种子发芽的影响[J]. 种子, 1986(Z1):62.
[21]	TROTEL P, BOUCHEREAU A, NIOGRET M F, et al. The fate of osmo-accumulated proline in leaf discs of rape (Brassica napus L.) incubated in a medium of low osmolarity[J]. Plant science, 1996, 118(1):31-45.
[22]	殷丽琴, 钟成, 陈伟, 等. 湿害胁迫下硒对油菜出苗率及叶绿素含量的影响[J]. 西南农业学报, 2013, 26(4):1398-1401.
[23]	卢长明, 龚学明. 缺氧条件下油菜种子发芽特性的遗传参数研究[J]. 江西农业大学学报, 1989(4):4.
[24]	丛野, 程勇, 邹崇顺, 等. 甘蓝型油菜发芽种子耐湿性的主基因+多基因遗传分析[J]. 作物学报, 2009, 35(8):1462-1467.
[25]	丛野. 甘蓝型油菜耐湿性的遗传分析及肥料调控研究[D]. 北京: 中国农业科学院, 2010.
[26]	张学昆, 陈洁, 王汉中, 等. 甘蓝型油菜耐湿性的遗传差异鉴定[J]. 中国油料作物学报, 2007(2):204-208.

品种	淹水时间/d	主根长/cm		株高/cm		整株鲜重/(g/株)		发芽率/%
品种	淹水时间/d	对照组	处理组	对照组	处理组	对照组	处理组	对照组	处理组
川油36	3	4.03	3.27^*	3.33	2.57^*	0.47	0.29^*	90	84^*
	5	5.30	4.62^*	4.47	3.80^*	0.51	0.37^*	90	65^**
	7	6.23	5.43^**	5.77	5.23^*	0.54	0.43^**	90	39^**
川油46	3	2.90	2.73	3.20	1.90^**	0.40	0.34^*	95	70^**
	5	5.63	3.37^**	4.40	2.97^*	0.55	0.48^*	95	48^**
	7	5.77	4.87^*	4.77	4.37	0.64	0.55^*	95	37^**
川油83	3	5.21	5.08	3.33	3.27	0.47	0.43	96	92
	5	5.13	4.50^*	4.17	3.33^*	0.52	0.47^*	96	87^*
	7	5.50	5.09^*	5.00	4.77^*	0.54	0.48^*	96	66^**
川早油1号	3	4.37	2.23^**	3.70	2.50^*	0.47	0.33^*	92	73^**
	5	6.30	4.83^**	4.23	3.67^*	0.49	0.42^*	92	57^**
	7	6.77	7.20^*	4.47	3.80^**	0.54	0.47^*	92	40^**
渝油35	3	3.50	3.20^*	3.87	2.47^*	0.38	0.31^*	94	62^**
	5	4.27	3.57^*	4.13	3.67^*	0.42	0.36^*	94	54^**
	7	6.43	5.33^**	5.73	4.13^**	0.50	0.40^**	94	32^**
春喜1402	3	2.67	3.47^*	2.83	2.03^*	0.39	0.33^*	95	75^**
	5	4.57	4.13^*	3.77	3.03^*	0.49	0.42^*	95	67^**
	7	4.97	4.30^*	5.33	4.10^*	0.56	0.50^*	95	41^**

品种	淹水时间/d	主根长/cm		株高/cm		整株鲜重/(g/株)		发芽率/%
品种	淹水时间/d	对照组	处理组	对照组	处理组	对照组	处理组	对照组	处理组
川油36	3	4.03	3.27^*	3.33	2.57^*	0.47	0.29^*	90	84^*
	5	5.30	4.62^*	4.47	3.80^*	0.51	0.37^*	90	65^**
	7	6.23	5.43^**	5.77	5.23^*	0.54	0.43^**	90	39^**
川油46	3	2.90	2.73	3.20	1.90^**	0.40	0.34^*	95	70^**
	5	5.63	3.37^**	4.40	2.97^*	0.55	0.48^*	95	48^**
	7	5.77	4.87^*	4.77	4.37	0.64	0.55^*	95	37^**
川油83	3	5.21	5.08	3.33	3.27	0.47	0.43	96	92
	5	5.13	4.50^*	4.17	3.33^*	0.52	0.47^*	96	87^*
	7	5.50	5.09^*	5.00	4.77^*	0.54	0.48^*	96	66^**
川早油1号	3	4.37	2.23^**	3.70	2.50^*	0.47	0.33^*	92	73^**
	5	6.30	4.83^**	4.23	3.67^*	0.49	0.42^*	92	57^**
	7	6.77	7.20^*	4.47	3.80^**	0.54	0.47^*	92	40^**
渝油35	3	3.50	3.20^*	3.87	2.47^*	0.38	0.31^*	94	62^**
	5	4.27	3.57^*	4.13	3.67^*	0.42	0.36^*	94	54^**
	7	6.43	5.33^**	5.73	4.13^**	0.50	0.40^**	94	32^**
春喜1402	3	2.67	3.47^*	2.83	2.03^*	0.39	0.33^*	95	75^**
	5	4.57	4.13^*	3.77	3.03^*	0.49	0.42^*	95	67^**
	7	4.97	4.30^*	5.33	4.10^*	0.56	0.50^*	95	41^**

品种	处理/d	主根长	株高	苗鲜重	发芽率	综合湿害指数	平均综合湿害指数
川油36	3	-0.19	-0.23	-0.38	-0.06	-0.86	-0.69
	5	-0.13	-0.15	-0.27	-0.06	-0.61
	7	-0.13	-0.09	-0.20	-0.18	-0.6
川油46	3	-0.46	-0.41	-0.15	-0.05	-1.07	-0.84
	5	-0.40	-0.33	-0.13	-0.07	-0.93
	7	-0.16	-0.08	-0.12	-0.15	-0.51
川油83	3	-0.02	-0.12	-0.11	-0.02	-0.27	-0.33
	5	-0.12	-0.08	-0.10	-0.03	-0.33
	7	-0.17	-0.05	-0.10	-0.08	-0.4
川早油1号	3	-0.32	-0.32	-0.30	-0.03	-0.97	-0.72
	5	-0.23	-0.13	-0.14	-0.11	-0.61
	7	-0.16	-0.12	-0.13	-0.17	-0.58
渝油35	3	-0.19	-0.36	-0.18	-0.01	-0.74	-0.55
	5	-0.16	-0.11	-0.14	-0.06	-0.47
	7	-0.13	-0.08	-0.10	-0.23	-0.44
春喜1402	3	-0.30	-0.28	-0.15	-0.08	-0.81	-0.64
	5	-0.13	-0.20	-0.14	-0.12	-0.59
	7	-0.10	-0.13	-0.11	-0.18	-0.52

品种	处理/d	主根长	株高	苗鲜重	发芽率	综合湿害指数	平均综合湿害指数
川油36	3	-0.19	-0.23	-0.38	-0.06	-0.86	-0.69
	5	-0.13	-0.15	-0.27	-0.06	-0.61
	7	-0.13	-0.09	-0.20	-0.18	-0.6
川油46	3	-0.46	-0.41	-0.15	-0.05	-1.07	-0.84
	5	-0.40	-0.33	-0.13	-0.07	-0.93
	7	-0.16	-0.08	-0.12	-0.15	-0.51
川油83	3	-0.02	-0.12	-0.11	-0.02	-0.27	-0.33
	5	-0.12	-0.08	-0.10	-0.03	-0.33
	7	-0.17	-0.05	-0.10	-0.08	-0.4
川早油1号	3	-0.32	-0.32	-0.30	-0.03	-0.97	-0.72
	5	-0.23	-0.13	-0.14	-0.11	-0.61
	7	-0.16	-0.12	-0.13	-0.17	-0.58
渝油35	3	-0.19	-0.36	-0.18	-0.01	-0.74	-0.55
	5	-0.16	-0.11	-0.14	-0.06	-0.47
	7	-0.13	-0.08	-0.10	-0.23	-0.44
春喜1402	3	-0.30	-0.28	-0.15	-0.08	-0.81	-0.64
	5	-0.13	-0.20	-0.14	-0.12	-0.59
	7	-0.10	-0.13	-0.11	-0.18	-0.52

	主根长	株高	苗鲜重	发芽率
株高	0.132
苗鲜重	0.336^**	0.254^**
发芽率	0.111^*	-0.299^*	0.128^*
综合湿害指数	0.685^**	0.550^**	0.585^**	0.642^**