Research Progress on Anthracnose and Its Pathogens in Amomi Fructus

doi:10.11924/j.issn.1000-6850.casb2023-0650

Abstract

Abstract:

Anthracnose, caused by Colletotrichum spp., is a major disease that significantly affects the safe production and quality of Amomi fructus. The research on the pathogenic bacteria causing anthracnose in Amomi fructus is currently inadequate. This paper provides an overview of research findings on the occurrence, harmful effects, prevention and control measures of anthracnose in Amomi fructus, as well as the characteristics in morphology and biology, classification, and identification and detection techniques of the pathogen. The aim is to serve as a reference for further basic research and control measures on Amomi fructus anthracnose.

Key words: Amomi fructus, anthracnose, Colletotrichum spp., fungal disease, prevention and control measures

DENG Quanqing, XU Shiqiang, MEI Yu, GU Yan, LI Jingyu, WANG Jihua. Research Progress on Anthracnose and Its Pathogens in Amomi Fructus[J]. Chinese Agricultural Science Bulletin, 2024, 40(17): 120-126.

Figures/Tables 4

References 60

[1]	SUO S, LAI Y, LI M, et al. Phytochemicals, pharmacology, clinical application, patents, and products of Amomi fructus[J]. Food and chemical toxicology: an international journal published for the British industrial biological research association, 2018,119:31-36.
[2]	赵宏友, 王延谦, 王艳芳, 等. 四大南药之一阳春砂栽培研究进展[J]. 世界中医药, 2022, 17(8):1163-1170.
[3]	林美珍, 陈荣珠, 赖钟雄, 等. 阳春砂花粉的结构和生殖细胞的分离[J]. 西北植物学报, 2019, 39(11):1998-2002.
[4]	申丽, 王洋, 江坤, 等. 阳春砂、绿壳砂与海南砂高效液相色谱指纹图谱比较研究[J]. 中国药学杂志, 2016, 51(12):1039-1043. doi: 10.11669/cpj.2016.12.017
[5]	刘信丹, 张英, 吴孟华, 等. 岭南药材道地性研究进展[J]. 中国中药杂志, 2019, 44(11):2185-2190.
[6]	YAO Y, MI W, CAO G, et al. The absorption characteristics of nonvolatile components in a water extraction from Amomi fructus as determined by In Situ single-pass intestinal perfusion and high-performance liquid chromatography[J]. Frontiers in pharmacology, 2020,11:711.
[7]	吕秉鼎, 胡佳佳, 汤丽云, 等. 阳春砂落果规律及其生理机制[J]. 植物生理学报, 2021, 57(2):429-438.
[8]	SONG L, LIN W, JIANG N, et al. Anthracnose disease of Amomum villosum caused by Colletotrichum fructicola in China[J]. Journal of general plant pathology, 2021,87:259-263.
[9]	杨梅仙. 阳春砂仁炭疽病的初步研究[J]. 植物保护, 1987, 6(6):21-22.
[10]	陈菁瑛, 陈景耀. 南方药用植物病虫害防治上[M]. 北京: 中国农业出版社,2017:2-3.
[11]	姜子德, 戚佩坤. 砂仁真菌病害的鉴定[J]. 华南农业大学学报, 1989, 10(01):9-16.
[12]	陈红梅, 余东, 罗虎, 等. 橡胶-砂仁间作土壤中细菌数量及其对病原菌拮抗性和作物促生性影响[J]. 热带农业科学, 2022, 42(12):1-7.
[13]	李学兰, 李荣英, 杨春勇. 西双版纳阳春砂仁叶枯病的调查及影响因素分析[J]. 中国农学通报, 2007, 23(3):424-427.
[14]	桑维钧, 宋宝安, 练启仙, 等. 砂仁炭疽病病原菌鉴定及杀菌剂毒力测定[J]. 农药, 2006, 45(8):558-560.
[15]	李向东, 杨明英, 曹继芬, 等. 砂仁叶斑病田间发病影响因素调查研究[J]. 西南农业学报, 2011, 24(4):1363-1366.
[16]	VILLA-RIVERA M G, CONEJO-SAUCEDO U, LARA-MARQUEZ A, et al. The role of virulence factors in the pathogenicity of Colletotrichum sp.[J]. Current protein and peptide science, 2017, 18(10):1005-1018.
[17]	刘丽萍, 高洁, 李玉. 植物炭疽菌属Colletotrichum真菌研究进展[J]. 菌物研究, 2020, 18(4):266-281.
[18]	洪祥千. 海南岛南药病原微生物鉴定的研究[J]. 热带作物学报, 1987, 8(1):121-132.
[19]	赖传雅, 梁钧, 韦刚. 阳春砂仁(Amomum villosum lour.)叶枯病菌基本生物学特性研究[J]. 植物病理学报, 1993, 23(4):7-12.
[20]	吴忠发. 姜科植物主要病害及防治[J]. 南方农业学报, 1998, 4(4):194-195.
[21]	韩凤, 肖杰易, 林茂祥. 砂仁属植物的病害及防治[J]. 中国现代中药, 2006, 8(4):35-36.
[22]	练启仙. 贵州花江峡谷砂仁等主要经济植物真菌病害种类调查及药剂筛选[D]. 贵阳: 贵州大学, 2006.
[23]	WEIR B S, JOHNSTON P R, DAMM U. The Colletotrichum gloeosporioides species complex[J]. Studies in mycology, 2012, 73(1):115-180.
[24]	LIU F, WEIR B S, DAMM U, et al. Unravelling Colletotrichum species associated with Camellia: employing ApMat and GS loci to resolve species in the C. gloeosporioides complex[J]. Persoonia: Molecular phylogeny and evolution of fungi, 2015, 35(1):63-86.
[25]	赵飞飞, 沈量, 傅敏, 等. 梨果生刺盘孢子囊孢子单孢菌系的生物学特性及致病性研究[J]. 植物病理学报, 2019, 49(1):1-10.
[26]	魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社,1979:463.
[27]	赖传雅. 阳春砂仁(Amomum villosum Lour.)叶枯病病原及寄主范围研究[J]. 广西农业大学学报, 1993, 12(1):49-56.
[28]	练启仙, 桑维钧, 李小霞, 等. 砂仁炭疽病病原菌生物学特性的研究[J]. 黔西南民族师范高等专科学校学报, 2007, 6(2):119-124.
[29]	姚权, 郭源, 魏丰园, 等. bZIP转录因子CfHac1参与调控果生刺盘孢菌的生长发育和致病力[J]. 菌物学报, 2019, 38(10):1643-1652. doi: 10.13346/j.mycosystema.190228
[30]	CANNON P F, DAMM U, JOHNSTON P R, et al. Colletotrichum-current status and future directions[J]. Studies in mycology, 2012, 73(1):181-213.
[31]	SAKINAH M A I, SUZIANTI I V, LATIFFAH Z. First report of Colletotrichum gloeosporioides causing anthracnose of banana (Musa spp.) in Malaysia[J]. Plant disease, 2013, 97(7):991-991.
[32]	NAZ F, ABBAS M F, RAUF C A, et al. First report of Colletotrichum gloeosporioides causing anthracnose on loquat in Pakistan[J]. Plant disease, 2017, 101(8):1550.
[33]	SHI Y X, ZHANG X H, ZHAO Q, et al. First report of Colletotrichum gloeosporioides causing anthracnose on okra in China[J]. Plant disease, 2019, 103(5):1023-1023.
[34]	CARBONE I, KOHN L M. A method for designing primer sets for speciation studies in filamentous ascomycetes[J]. Mycologia, 1999, 91(3):553-55
[35]	GUERBER J C, LIU B, CORRELL J C, et al. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility[J]. Mycologia, 2003, 95(5):872-895.
[36]	STEPHENSON S A, GREEN J R, MANNERS J M, et al. Cloning and characterisation of glutamine synthetase from Colletotrichum gloeosporioides and demonstration of elevated expression during pathogenesis on Stylosanthes guianensis[J]. Current genetics, 1997, 31(5):447-454
[37]	WHITE T J, BRUNS T, LEE S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis M A, GELFAND D H, SNINSKY J J, WHITE T J, eds., PCR protocols: a guide to methods and applications[M]. London: Academic Press,1990:315-322
[38]	O’DONNELL K, CIGELNIK E. Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous[J]. Molecular phylogenetics and evolution, 1997, 7(1):103-116 doi: 10.1006/mpev.1996.0376 pmid: 9007025
[39]	GLASS N L, DONALDSON G C. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes[J]. Applied and environmental microbiology, 1995, 61(4):1323-1330 doi: 10.1128/aem.61.4.1323-1330.1995 pmid: 7747954
[40]	MORIWAKI J, TSUKIBOSHI T. Colletotrichum echinochloae, a new species on Japanese barnyard millet (Echinochloa utilis)[J]. Mycoscience, 2009, 50(4):273-280
[41]	宋晓兵, 黄峰, 罗小玲, 等. 吡唑醚菌酯对两种优稀水果病原菌的毒力测定及田间防治效果[J]. 中国农学通报, 2022, 38(27):125-128. doi: 10.11924/j.issn.1000-6850.casb2021-0996
[42]	张丹雁, 刘军民, 徐鸿华. 阳春砂的病虫害调查与防治[J]. 现代中药研究与实践, 2005, 19(4):15-17.
[43]	张亚楠, 陈新慧, 张杰. 苹果炭疽病抗性miRNA的筛选[J]. 中国农学通报, 2021, 37(7):106-111. doi: 10.11924/j.issn.1000-6850.casb2020-0519
[44]	解娜, 李荣, 易图永. 一株拮抗辣椒炭疽病菌的放线菌筛选及鉴定[J]. 中国农学通报, 2013, 29(10):150-153.
[45]	吉沐祥, 杨敬辉, 吴祥, 等. 草莓炭疽病的生物防治[J]. 江苏农业学报, 2012, 28(6):1569-1571.
[46]	章乐乐, 王冠, 柳凤, 等. 芒果炭疽病拮抗菌分离、鉴定及生防机制研究[J]. 生物技术通报, 2023, 39(4):277-287. doi: 10.13560/j.cnki.biotech.bull.1985.2022-0908
[47]	PATIÑO-VERA M, JIMÉNEZ B, BALDERAS K, et al. Pilot-scale production and liquid formulation of Rhodotorula minuta, a potential biocontrol agent of mango anthracnose[J]. Journal of applied microbiology, 2005, 99(3):540-550.
[48]	范煜, 何桢锐, 黄晓彤, 等. 基于多组学的真菌病毒与寄主真菌互作的研究进展[J]. 热带生物学报, 2023, 14(4):399-404,440.
[49]	时婷婷, 覃茜, 张君成. 真菌病毒对植物病原物的生物防治作用及其研究进展[J]. 安徽农业科学, 2015, 43(3):131-132,162.
[50]	刘昌芬. 主要热带和亚热带经济作物的胶孢炭疽菌. 云南热作科技, 1995, 18(4):27-29.
[51]	李荣英, 彭建明, 高微微. 阳春砂仁植株新株萌发与叶枯病发生的关系[J]. 中国中药杂志, 2009, 34(12):1588-1590.
[52]	汪增丰, 刘付月清, 林思诚, 等. 林下春砂仁的种植技术要点[J]. 南方农业, 2018, 12(21):22-23.
[53]	赖传雅. 阳春砂仁叶枯病的发生与防治[J]. 广西植保, 1994(4):38-39.
[54]	王群, 毕云青, 何成兴, 等. 半边红李炭疽病病害调查及病原菌鉴定[J]. 中国农学通报, 2021, 37(15):128-136. doi: 10.11924/j.issn.1000-6850.casb2020-0328
[55]	O’CONNELL R, HERBERT C, SREENIVASAPRASAD S, et al. A novel Arabidopsis-Colletotrichum pathosystem for the molecular dissection of plant-fungal interactions[J]. Molecular plant-microbe interactions, 2004, 17(3):272-282
[56]	O’CONNELL RJ, THON MR, HACQUARD S, et al. Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses[J]. Nature genetics, 2012, 44(9):1060-1065.
[57]	YANG P, ZHAO H Y, WEI J S, et al. Chromosome-level genome assembly and functional characterization of terpene synthases provide insights into the volatile terpenoid biosynthesis of Wurfbainia villosa[J]. Plant journal, 2022, 112(3):630-645.
[58]	CHEN X, SUN S, HAN X, et al. Multiomics comparison among populations of three plant sources of Amomi Fructus[J]. Horticulture research, 2023, 10(8):uhad128.
[59]	王玉春, 刘守安, 卢秦华, 等. 中国茶树炭疽菌属病害研究进展及展望[J]. 植物保护学报, 2019, 46(5):954-963.
[60]	LI J, SUN K, MA Q P, et al. Colletotrichum gloeosporioides-contaminated tea infusion blocks lipids reduction and induces kidney damage in mice[J]. Frontiers in microbiology, 2017,8:2089.

基因	引物名称	序列(5'-3')	参考文献
ACT	ACT-512F	ATGTGCAAGGCCGGTTTCGC	[34]
ACT	ACT-783R	TACGAGTCCTTC GGCCCAT	[34]
CAL	CL1C	GAATTCAAGGAGGCCTTCTC	[23]
CAL	CL2C	CTTCTGCATCATGAGCTGGAC	[23]
CHS-1	CHS-79F	TGGGGCAAGGATGCTTGGAAGAAG	[34]
CHS-1	CHS-345R	TGGAAGAACCATCTGTGAGAGTTG	[34]
GAPDH	GDF1	GCCGTCAACGACCCCTTCATTGA	[35]
GAPDH	GDR1	GGGTGGAGTCGTACTTGAGCATGT	[35]
GS	GSF1	ATGGCCGAGTACATCTGG	[36]
GS	GSR1	GAACCGTCGAAGTTCCAG	[36]
ITS	ITS-4	CTTGGTCATTTAGAGGAAGTAA	[37]
ITS	ITS-5	TCCTCCGCTTATTGATATGC	[37]
TUB2	T1	AACATGCGTGAGATTGTAAGT	[38]
TUB2	Bt2b	TAGTGACCCTTGGCCCAGTTG	[39]
SOD2	SODglo2-F	CAGATCATGGAGCTGCACCA	[40]
SOD2	SODglo2-R	TAGTACGCGTGCTCGGACAT	[40]

基因	引物名称	序列(5'-3')	参考文献
ACT	ACT-512F	ATGTGCAAGGCCGGTTTCGC	[34]
ACT	ACT-783R	TACGAGTCCTTC GGCCCAT	[34]
CAL	CL1C	GAATTCAAGGAGGCCTTCTC	[23]
CAL	CL2C	CTTCTGCATCATGAGCTGGAC	[23]
CHS-1	CHS-79F	TGGGGCAAGGATGCTTGGAAGAAG	[34]
CHS-1	CHS-345R	TGGAAGAACCATCTGTGAGAGTTG	[34]
GAPDH	GDF1	GCCGTCAACGACCCCTTCATTGA	[35]
GAPDH	GDR1	GGGTGGAGTCGTACTTGAGCATGT	[35]
GS	GSF1	ATGGCCGAGTACATCTGG	[36]
GS	GSR1	GAACCGTCGAAGTTCCAG	[36]
ITS	ITS-4	CTTGGTCATTTAGAGGAAGTAA	[37]
ITS	ITS-5	TCCTCCGCTTATTGATATGC	[37]
TUB2	T1	AACATGCGTGAGATTGTAAGT	[38]
TUB2	Bt2b	TAGTGACCCTTGGCCCAGTTG	[39]
SOD2	SODglo2-F	CAGATCATGGAGCTGCACCA	[40]
SOD2	SODglo2-R	TAGTACGCGTGCTCGGACAT	[40]