Inhibitory Activity and Mechanism of Honokiol Against Chinese Cabbage Soft Rot

doi:10.11924/j.issn.1000-6850.casb2025-0325

Abstract

Abstract:

This study aimed to investigate the antibacterial activity and mechanism of honokiol against Pectobacterium carotovorum subsp. carotovorum (Pcc). This experiment used Pcc as the test strain and honokiol as the test compound, to determine the antibacterial effect of honokiol against Pcc using the inhibition zone method and turbidimetric method, and explored its antibacterial mechanism by testing cell wall integrity, cell membrane integrity and permeability, and respiratory activity. The results of the antibacterial experiment showed that the inhibition zone diameter of 10 mg/mL honokiol was 23.71±0.86 mm, and its minimum inhibitory concentration value was 0.0156 mg/mL. After honokiol treatment, extracellular alkaline phosphatase (AKP) activity, propidium iodide (PI) fluorescence intensity, extracellular nucleic acid leakage, and protein leakage increased significantly (P<0.05), whereas cell membrane potential and total ATPase (T-ATPase) activity decreased significantly (P<0.05).These results indicated that honokiol exhibited significant antibacterial activity against Pcc by disrupting cell wall and membrane structures, increasing membrane permeability, and inhibiting respiratory function.

Key words: plant-derived fungicide, honokiol, soft rot of Chinese cabbage, antibacterial activity, antibacterial mechanism

WANG Jiafei, LYU Hangjun, XIE Jiale, CAI Jin. Inhibitory Activity and Mechanism of Honokiol Against Chinese Cabbage Soft Rot[J]. Chinese Agricultural Science Bulletin, 2025, 41(29): 99-106.

Figures/Tables 9

References 39

[1]	王敏, 宋雨萌, 刘文杰, 等. 7种药剂对白菜黑斑病菌的毒力及增效配方筛选[J]. 现代农药, 2023, 22(1):64-67.
[2]	LI X, REN X, IBRAHIM E, et al. Response of Chinese cabbage (Brassica rapa subsp. pekinensis) to bacterial soft rot infection by change of soil microbial community in root zone[J]. Frontiers in microbiology, 2024, 15:1401896.
[3]	程凯凯, 王秀艳, 郭周倩, 等. 白菜软腐病病原菌鉴定及地衣芽孢杆菌XW02对其的抑制作用[J]. 河北农业科学, 2024, 28(4):49-53,58.
[4]	YAN S, HU Q, JIANG Q, et al. Simple osthole/nanocarrier pesticide efficiently controls both pests and diseases fulfilling the need of green production of strawberry[J]. ACS applied materials & interfaces, 2021, 13(30):36350-36360.
[5]	赫卫, 潘嘉仪, 罗梅, 等. 三种中草药提取物对植物病原菌的抑菌活性[J]. 热带农业科学, 2024, 44(12):90-95.
[6]	张超, 文庭池, 曾祥羽, 等. 和厚朴酚对红托竹荪腐烂病菌抑菌活性的影响[J]. 北方园艺, 2024(21):92-98.
[7]	赵霞, 周玉惠, 李婷, 等. 植物源农药萜烯醇对3种作物病害的毒力与防治效果研究[J]. 农学学报, 2025, 15(2):43-47. doi: 10.11923/j.issn.2095-4050.cjas2023-0276
[8]	齐泽铮, 于璐, 郭旭语, 等. 甜瓜茎基腐病病原菌鉴定及其植物源杀菌剂室内毒力测定[J]. 黑龙江农业科学, 2024(8):33-38.
[9]	王昆, 田家顺. 我国生物农药登记现状及应用[J]. 现代农业科技, 2024(17):101-103,107.
[10]	QUIDEAU S, DEFFIEUX D, DOUAT-CASASSUS C, et al. Plant polyphenols: chemical properties, biological activities, and synthesis[J]. Angewandte chemie international edition, 2011, 50(3):586-621.
[11]	DAGLIA M. Polyphenols as antimicrobial agents[J]. Current opinion in biotechnology, 2012, 23(2):174-181. doi: 10.1016/j.copbio.2011.08.007 pmid: 21925860
[12]	张明发, 沈雅琴. 中药厚朴及其有效成分厚朴酚与和厚朴酚抗真菌和病毒的药理作用研究进展[J]. 抗感染药学, 2021, 18(12):1724-1728. doi: 10.13493/j.issn.1672-7878.2021.12-002
[13]	杨康, 余苗, 尹福泉, 等. 和厚朴酚的生物学功能及其饲用化前景的研究[J]. 动物营养学报, 2024, 36(4):2163-2174. doi: 10.12418/CJAN2024.188
[14]	MA D, CUI X, ZHANG Z, et al. Honokiol suppresses mycelial growth and reduces virulence of Botrytis cinerea by inducing autophagic activities and apoptosis[J]. Food microbiology, 2020, 88:103411.
[15]	YAN Y F, YANG C J, SHANG X F, et al. Bioassay-guided isolation of two antifungal compounds from Magnolia officinalis, and the mechanism of action of honokiol[J]. Pesticide biochemistry and physiology, 2020, 170:104705.
[16]	黄红琴, 冉小青, 杨欢, 等. 厚朴中厚朴酚与和厚朴酚的超临界CO₂提取工艺优化[J]. 中成药, 2024, 46(5):1636-1639.
[17]	马宁, 邢怡桥. 和厚朴酚最新药理学研究进展[J]. 时珍国医国药, 2018, 29(12):3007-3009.
[18]	王晓芸, 张婷, 黄剑, 等. 3种多酚对腐败希瓦氏菌的抑菌效果和机理[J]. 食品科学, 2024, 45(16):1-9.
[19]	石超, 郭都, 张文婷, 等. 原儿茶醛对阪崎克罗诺肠杆菌的抑制作用及机理[J]. 现代食品科技, 2017, 33(7):105-111,62.
[20]	FAN W W, YUAN G Q, LI Q Q, et al. Antibacterial mechanisms of methyl gallate against Ralstonia solanacearum[J]. Australasian plant pathology, 2014, 43:1-7.
[21]	徐德强, 王英明, 周德庆. 微生物学实验教程[M]. 北京: 高等教育出版社, 2019:65-73.
[22]	BALOUIRI M, SADIKI M, IBNSOUDA S K. Methods for in vitro evaluating antimicrobial activity: a review[J]. Journal of pharmaceutical analysis, 2016, 6(2):71-79.
[23]	李兴海, 李北兴. 农药学实验技术与指导[M]. 北京: 化学工业出版社出版, 2023:186-215.
[24]	PARK J H, KIM S, CHANG Y, et al. Synergistic antimicrobial effect and mode of action of palmarosa oil-loaded nanoemulsion and citric acid against Pectobacterium carotovorum[J]. Food science and biotechnology, 2023, 32(6):823-831.
[25]	李婷, 周月, 宋丽雅, 等. 植物抑菌剂抑菌机理的研究方法进展[J]. 北方园艺, 2016(4):179-184.
[26]	陈晓慧, 王桂琴, 王建崇, 等. 肉桂醛与麝香草酚对金黄色葡萄球菌的协同抑菌活性及作用机制[J]. 中国畜牧兽医, 2024, 51(12):5528-5538. doi: 10.16431/j.cnki.1671-7236.2024.12.039
[27]	禄锦鹏, 刘文波, 范鸿雁, 等. 丁香酚对合果芋果胶杆菌(Pectobacterium aroidearum) L6抑菌效果研究[J]. 安徽农业科学, 2025, 53(4):124-128.
[28]	欧凯玉, 逄建龙, 张一敏, 等. 天然酚类化合物的抑菌作用及在肉与肉制品中的应用研究进展[J]. 食品科学, 2023, 44(9):358-366.
[29]	王江来, 张锦锋, 马金秀, 等. 香芹酚和丁香酚对腐皮镰刀菌的抑菌活性及抑菌机理[J]. 微生物学通报, 2022, 49(5):1638-1650.
[30]	WANG X, XIE Y, LI Z, et al. Honokiol inhibits growth and improves control efficiency against Phytophthora nicotianae through disrupting redox homeostasis and energy metabolism[J]. Industrial crops and products, 2022, 178:114656.
[31]	ZHANG Z, CHEN T, YIN X, et al. Honokiol inhibits Botryosphaeria dothidea, the causal pathogen of kiwifruit soft rot, by targeting membrane lipid biosynthesis[J]. Pest management science, 2024, 80(4):1779-1794.
[32]	LIU X, CAI J, CHEN H, et al. Antibacterial activity and mechanism of linalool against Pseudomonas aeruginosa[J]. Microbial pathogenesis, 2020, 141:103980.
[33]	刘静, 谢朋飞, 蔡延渠. 茶皂素对临床常见致病菌的抗菌活性及作用机制研究[J]. 广东药科大学学报, 2021, 37(5):35-41.
[34]	SULISTYANI N, MAHFUDH N, MAWARDI R H, et al. Cell leakage mechanism and time kill studies on Staphylococcus aureus after exposure to ethanol leaf extract of Muntingia calabura L.[J]. Tropical journal of pharmaceutical research, 2023, 22(2):355-362.
[35]	冯云芳, 冯志宏, 张新宪, 等. 连翘生物保鲜剂对冬枣采后链格孢菌的抑菌机理[J]. 食品与发酵工业, 2025, 51(2):176-184. doi: 10.13995/j.cnki.11-1802/ts.038596
[36]	费鹏, 赵胜娟, 陈曦, 等. 植物多酚抑菌活性、作用机理及应用研究进展[J]. 食品与机械, 2019, 35(7):226-230.
[37]	姚文兵. 生物化学[M]. 北京: 人民卫生出版社, 2016:162-179.
[38]	周本宏, 松长青, 姜姗, 等. 地榆鞣质提取物的抗菌活性及对金黄色葡萄球菌的抑菌机制研究[J]. 中国药师, 2016, 19(3):464-469.
[39]	符莎露, 吴甜甜, 吴春华, 等. 植物多酚的抗氧化和抗菌机理及其在食品中的应用[J]. 食品工业, 2016, 37(6):242-246.

杀菌剂	浓度/(mg/mL)										3%DMSO
杀菌剂	2.0000	1.0000	0.5000	0.2500	0.1250	0.0625	0.0312	0.0156	0.0078	0.0039	3%DMSO
和厚朴酚	-	-	-	-	-	-	-	-	+	+++	+++
噻唑锌	-	-	-	-	-	+	+++	+++	+++	+++	+++

杀菌剂	浓度/(mg/mL)										3%DMSO
杀菌剂	2.0000	1.0000	0.5000	0.2500	0.1250	0.0625	0.0312	0.0156	0.0078	0.0039	3%DMSO
和厚朴酚	-	-	-	-	-	-	-	-	+	+++	+++
噻唑锌	-	-	-	-	-	+	+++	+++	+++	+++	+++