Abstract:

In order to develop the utilization of wild edible mushroom resources in Tibet, this study isolated and identified a strain coded X21108 collected from Zhamu Town, Bomê County, Tibet. Based on ITS sequence analysis and the construction of a phylogenetic tree using the maximum likelihood method, the strain was identified as Peniophora incarnata. The mycelial growth rate was used as an indicator to investigate the optimal carbon source, nitrogen source, pH, and temperature for the strain in order to study its biological characteristics. The results indicated that in terms of biological characteristics the optimal carbon source for the mycelial growth of this strain was sucrose, under which the mycelial growth rate reached 6.93 ± 0.38 mm/d; using ammonium nitrate as the nitrogen source, the mycelial growth rate of Peniophora incarnata was the fastest, reaching 7.35 ± 0.22 mm/d; the optimal pH and temperature were 5.0 and 25℃, with the mycelial growth rates being 6.05±0.42 mm/d and 8.22 ± 0.54 mm/d, respectively. The results of biological characteristics showed that Peniophora incarnata X21108 was a medium-temperature strain with acidic growth environment. In terms of domestication, the formation of primordia was formed after 10 days of cultivation on a substrate consisting of coarse wood chips (40%), fine wood chips (20%), cottonseed hulls (20%), wheat bran (18%), lime (1%), and sucrose (1%), and mushrooms could be harvested after 50 days, resulting in the formation of Peniophora incarnata fruiting bodies. In summary, this study on the molecular identification, biological characteristics, and domestication cultivation of wild mushroom resources in Tibet had important practical significance for the development and utilization of wild edible mushroom resources.

Key words: Tibetan wild edible fungi, Peniophora incarnata, ITS sequence analysis, molecular identification, the phylogenetic tree, biological characteristics, domestication cultivation