Effects of Different Fertilization Practices on Organic Carbon and Enzyme Activity in Soil of Continuous Cropping Celery

doi:10.11924/j.issn.1000-6850.casb2025-0598

Abstract

Abstract:

To study the influence of fertilization measures on the variation of soil organic carbon and enzyme activity, and to provide theoretical basis for rational application of organic fertilizer to continuous cropping celery fields in the mountainous area of southern Ningxia, field trials were conducted from 2019 to 2024 in long-term continuous cropping celery fields in Xiji, Ningxia. The experiment was set up in a randomized block design with four fertilization modes: i.e., no fertilizer (CK), organic fertilizer (OF), biochar (BC), and biochar + organic fertilizer dosing (BF). By measuring soil organic carbon content, active organic carbon fractions, urease activity, and sucrase activity, this study investigated the effects of fertilization practices on changes of soil organic carbon and enzyme activity. The results showed that compared with CK, BF and OF were able to significantly increase the mass fraction of clusters with >1 mm size and the mean mass diameter (MWD) and geometric mean diameter (GMD) of the clusters. In >1mm and <0.25mm grain sizes, the soil organic carbon levels of BF treatment were higher than those of other treatments. In 0.25-1mm and <0.25mm grain sizes, the CPMI of OF treatment was significantly more than the other processing, which increased the CPMI by 2.39% and 21.15% compared with CK. BC treatment had the highest urease activity in all grain level agglomerates, with a significant improvement of 34.85%, 22.83% and 0.39% over CK, followed by OF treatment. The magnitude of soil sucrase activity in all grain level agglomerates showed that OF>CK>BC>BF, and OF increased by 10.51%, 10.59%, and 3.19% compared with CK. Correlation analysis results indicated that across all aggregate size fractions, soil urease activity exhibited a negative correlation with soil active organic carbon; sucrase activity showed a positive correlation with urease activity. And in the >1mm and 0.25-1mm agglomerates, the organic carbon was positively linked to soil activated organic carbon compositions, and significantly negatively linked to the activities of sucrase. In summary, the application of organic fertilizers can improve soil structure, enhance aggregate stability, increase the content of soil organic carbon (SOC) and active organic carbon components in particle size fractions >1 mm and <0.25 mm, elevate the level of soil carbon pools, increase the enzyme activity of the aggregates, and improve the quality of the soil carbon pool levels, and boost aggregate enzyme activity, thereby effectively improving soil quality of continuous cropping celery fields.

Key words: organic fertilizer, biochar, organic carbon, soil carbon pool management index, enzyme activity

CLC Number:

S3，S5

JIN Xuelan, HU Shengyuan, SHI Lin, MA Yaoyu, YANG Ting, WU Hongliang, KANG Jianhong, FENG Haiping. Effects of Different Fertilization Practices on Organic Carbon and Enzyme Activity in Soil of Continuous Cropping Celery[J]. Chinese Agricultural Science Bulletin, 2026, 42(8): 120-127.

Figures/Tables 9

References 37

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处理	各粒级团聚体质量分数
处理	>1 mm	0.25~1 mm	<0.25 mm
CK	46.45±4.10b	32.73±0.81a	20.82±2.17c
BC	42.32±1.19c	31.35±2.46b	26.34±1.03a
OF	52.29±1.14a	29.47±1.32b	18.24±2.27d
BF	51.45±1.66ab	26.73±2.91c	21.82±4.53b

处理	各粒级团聚体质量分数
处理	>1 mm	0.25~1 mm	<0.25 mm
CK	46.45±4.10b	32.73±0.81a	20.82±2.17c
BC	42.32±1.19c	31.35±2.46b	26.34±1.03a
OF	52.29±1.14a	29.47±1.32b	18.24±2.27d
BF	51.45±1.66ab	26.73±2.91c	21.82±4.53b

项目	PC1	PC2
特征值	2.154	1.034
方差贡献率/%	53.857	25.83
累计方差贡献率/%	53.857	79.70

项目	PC1	PC2
特征值	2.154	1.034
方差贡献率/%	53.857	25.83
累计方差贡献率/%	53.857	79.70

处理	土壤质量指数SQI	排序
OF	0.641	1
CK	0.610	2
BC	0.588	3
BF	0.529	4