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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (30): 231-233.doi: 10.11924/j.issn.1000-6850.casb14110083

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PCR Detection of Sorghum mosaic virus (SrMV) in Sugarcane Field of Guizhou

Zhou Yufei, Fu Yuhua, Lei Shifu, Lu Jiaju   

  1. (Guizhou Institute of Subtropical Crops, Xingyi Guizhou 562400)
  • Received:2014-11-14 Revised:2015-08-05 Accepted:2015-08-10 Online:2015-10-29 Published:2015-10-29

Abstract: The study aims to find out mosaic disease epidemic situation in main sugarcane field in Guizhou. In this research, 52 sugarcane leaf samples with disease symptom from the main sugarcane field in Guizhou were detected by PCR. The results showed that 39 sugarcane samples were positive (75% samples were infected with SrMV). By sequencing the PCR amplified products of positive samples, the sequence homology of the PCR amplified product with SrMV isolates genes in GenBank was 96%-99%, thus confirmed the widespread infection of SrMV in main sugarcane field in Guizhou.

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