Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (30): 149-154.doi: 10.11924/j.issn.1000-6850.casb15040052

Special Issue: 生物技术 园艺

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Effect of Different Nitrogen Application Levels on the Content of Soluble Proteinand Key Enzyme Activities in Nitrogen Metabolism of Sugar Beet

Liu Na1,2,3, Yan Zhishan1,2,3, FanYoujun1,2,3, Song Baiquan1,2,3, Jia Hailun1,2,3, Yang Ji1,2,3   

  1. (1Crop Academy of Heilongjiang University, Harbin 150080;2Sugar Beet Research Institute Chinese Academy of Agricultural Sciences, Harbin 150080;3Key Laboratory of North Sugar Crop Resource and Utilization, Chinese Academy of Agricultural Sciences, Harbin 150080)
  • Received:2015-04-07 Revised:2015-09-30 Accepted:2015-04-22 Online:2015-10-29 Published:2015-10-29

Abstract: Urea was used as soil nitrogen fertilizer and HI003 was selected as experimental sample. In order to select suitable levels of nitrogen and study the relationship between key enzyme activities in nitrogen metabolism and nitrogen application levels of sugar beet, studies on the content of soluble protein, activities of nitrate reductase and glutamine synthase were conducted in roots and leaves of sugar beet with different nitrogen levels. Results of this experiment were as following: in sugar beet leaves, activities of nitrate reductase increased with the increase of nitrogen levels, but nitrate reductase activities decreased when nitrogen levels were more than 200 kg/hm2. Activities of nitrate reductase nitrogen levels increased with the increase of nitrogen levels in roots, and decreased with nitrogen levels more than 160 kg/hm2. In the whole growth period, glutamine synthase activities increased with nitrogen application in roots and leaves. The studies found that nitrogen levels and activities of nitrate reductase and glutamine synthase had significantly positive correlation in the early growth stage. In sugar beet leaves, the content of soluble protein was positive correlated with nitrogen levels on July 15th and on September 15th. The content of soluble protein and nitrogen levels had significantly positive correlation in roots in the whole growth period of sugar beet.

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