Abstract: To improve the rice blast resistance of thermo- sensitive genic male sterile line‘Q211S’, the thermo-sensitive genic male sterile line‘Q211S’was used as the receptor parent and the‘75-1-127’with excellent rice blast resistance, carrying genePi9 , was used as donor parent, five improved two-line male sterile lines with rice blast resistance were bred by using conventional breeding and molecular marker- assisted selection. Resistance identification and field trials demonstrated that the five improved two-line male sterile lines performed higher resistance to rice leaf blast and panicle neck blast, the other agronomic characters were almost identical to that of‘Q211S’. Furthermore, the new hybrid rice combinations which the five improved two-line male sterile line rice were used as female parents had no significant differences in plant height, spikelet number per panicle, setting rate and 1000- grain weight compared with that of‘Q211S’used as female parent, whereas the rice blast resistances of the new hybrid rice combinations which the five improved two-line male sterile line rice were used as female parents, were obviously enhanced