Cluster Analysis of Anti-brown Spot Sugar Beet Varieties with SSR Makers

doi:10.11924/j.issn.1000-6850.casb18060131

Abstract

Abstract: To breed and identify domestic sugar beet varieties’resistance to brown spot, and analyze the genetic relationship among the varieties with anti-brown spot disease, 14 pairs SSR molecule markers were used to study the relationship of 17 domestic varieties. Genetic distance and cluster diagram were obtained using MEGA 3.1 software and EXCEL 2007. Combining molecular markers and field survey results, antibrown spot disease investigation and cluster analysis were conducted. The results of molecular markers showed that a total of 99 bands were obtained, 81 of which were polymorphic. The average polymorphic ratio was 81.8%. The mean genetic distance was 0.4601, and genetic similarity among varieties was 0.6312. At a genetic distance of 0.2, the 17 genotypes were divided into five cluster groups based on cluster analysis, and the first group includes two sub-categories. Molecular markers showed that the genetic diversity of the 17 materials were rich. Field survey results showed that 5 varieties had better resistance to brown spot. The results of clustering showed that SSR molecular markers were effective in genetic analysis of anti-brown spot varieties and clustering distribution of sugar beet.

CLC Number:

S566.3

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