Abstract: [Objective] In order to understand the soil fungal community structure characteristics of different forest types in Tangwang River National Park, and the relationships of soil fungal community structures and soil environmental factors, [Method] the three forest type soils (0~20 cm soil layer) that took from the Brich secondary forest, Broad-leaved Korean pine forest and Spruce pine red pine forest were selected and sequenced the fungal community diversity by using the high-throughput sequencing technology. [Result]The results showed that the contents of soil organic carbon, total nitrogen, alkali nitrogen, available phosphorus and available potassium increased but the soil pH decreased from broad-leaved forest type to coniferous forest type. The soil fungi taxon included 3 phyla, 9 classes, 31 orders, 64 families, 82 genera and 75 species. The Basidiomycota was the dominant phylum in the Spruce forest and the Broad-leaved Korean pine forest. The Mucoromycota was the dominant phylum in the Birch secondary forest type. The relative abundance of Russula, Mortierella, Cenococcum, Pyrenochaetopsis, Solicoccozyma, Saitozyma, Humicolopsis and Tylospora differed significantly. [Conclusion] Based on the redundancy analysis and correlation analysis, the soil fungal diversity of different forest types in Tangwanghe National Park were affected by soil pH, total nitrogen, available nitrogen and moisture content.