Taurine Affecting Growth Performance, Non-specific Immunity and Antioxidative Competence of Procambarus clarkii

doi:10.11924/j.issn.1000-6850.casb20191100872

Abstract

Abstract:

This study aims to investigate the effect of taurine on the growth performance, non-specific immunity and antioxidative competence of Procambarus clarkii. 300 juvenile red swamp crayfish Procambarus clarkii with initial body weight of (4.50±0.50) g were selected and randomly divided into five groups. The five groups were fed with five isonitrogenous and isoenergetic diets with different taurine contents respectively. After feeding for 4 weeks, we investigated the growth performance, biochemical parameters of blood and liver. In 200 and 300 mg/kg treatment groups, some indices were significantly higher than those of the control group (P<0.05), such as the weight gain, dressing percentage, the levels of total protein (TP), lysozyme (LSZ), and the activities of superoxide dismutase (SOD) and catalase (CAT) in liver. In 200 and 300 mg/kg treatment groups, serum glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) were lower than those of the control group (P <0.05), which had the statistical difference. In 300 mg/kg treatment group, the activities of GSH-Px and survival rates were significantly higher than those of the control group (P <0.05), feed coefficient was significantly lower than that of the control group (P<0.05). However, there was no statistical difference in the contents of malondialdehyde (MDA) among the five groups. Therefore, the diet supplemented with 200-300 mg/kg taurine could promote the growth of Procambarus clarkii, improve the non-specific immunity and antioxidative competence, and enhance its disease resistance.

Key words: Procambarus clarkii, taurine, growth performance, immunity, antioxidative

CLC Number:

S963.73

Kong Shengchao, Xiao Peizhen, Zhu Zhiqiang, Wang Shuang. Taurine Affecting Growth Performance, Non-specific Immunity and Antioxidative Competence of Procambarus clarkii[J]. Chinese Agricultural Science Bulletin, 2020, 36(11): 136-141.

Figures/Tables 4

References 30

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原料	0 mg/kg组	100 mg/kg组	200 mg/kg组	300 mg/kg组	400 mg/kg组
超级蒸汽鱼粉	2	2	2	2	2
豆粕	30	30	30	30	30
菜粕	33	33	33	33	33
白酒渣	2	2	2	2	2
面粉	13	13	13	13	13
油糠	4.8	4.8	4.8	4.8	4.8
鱿鱼膏	4	4	4	4	4
磷脂油	2	2	2	2	2
豆油	2	2	2	2	2
磷酸二氢钙	2	2	2	2	2
膨润土	2	2	2	2	2
海藻酸钠	2	2	2	2	2
牛磺酸	0	0.01	0.02	0.03	0.04
谷氨酸	0.04	0.03	0.02	0.01	0
VC酯(35%型)	0.06	0.06	0.06	0.06	0.06
防霉剂	0.1	0.1	0.1	0.1	0.1
小龙虾复合预混料	1	1	1	1	1
总计	100	100	100	100	100
粗蛋白质/%	30.2	30.2	30.2	30.2	30.2
总脂肪/%	6.1	6.1	6.1	6.1	6.1
赖氨酸/%	1.6	1.6	1.6	1.6	1.6
灰分/%	9.4	9.4	9.4	9.4	9.4
水分/%	8.8	8.8	8.8	8.8	8.8

原料	0 mg/kg组	100 mg/kg组	200 mg/kg组	300 mg/kg组	400 mg/kg组
超级蒸汽鱼粉	2	2	2	2	2
豆粕	30	30	30	30	30
菜粕	33	33	33	33	33
白酒渣	2	2	2	2	2
面粉	13	13	13	13	13
油糠	4.8	4.8	4.8	4.8	4.8
鱿鱼膏	4	4	4	4	4
磷脂油	2	2	2	2	2
豆油	2	2	2	2	2
磷酸二氢钙	2	2	2	2	2
膨润土	2	2	2	2	2
海藻酸钠	2	2	2	2	2
牛磺酸	0	0.01	0.02	0.03	0.04
谷氨酸	0.04	0.03	0.02	0.01	0
VC酯(35%型)	0.06	0.06	0.06	0.06	0.06
防霉剂	0.1	0.1	0.1	0.1	0.1
小龙虾复合预混料	1	1	1	1	1
总计	100	100	100	100	100
粗蛋白质/%	30.2	30.2	30.2	30.2	30.2
总脂肪/%	6.1	6.1	6.1	6.1	6.1
赖氨酸/%	1.6	1.6	1.6	1.6	1.6
灰分/%	9.4	9.4	9.4	9.4	9.4
水分/%	8.8	8.8	8.8	8.8	8.8

牛磺酸添加量/(mg/kg)	终末体质量/g	增重率/%	成活率/%	出肉率/%	饵料系数
0	30.65±1.53^b	581.23±28.7^b	78.33±4.83^b	8.92±0.71^b	2.12±0.13^a
100	32.71±2.03^ab	626.43±19.1^ab	85.00±4.87^ab	9.13±1.21^b	2.07±0.21^a
200	35.81±1.87^a	681.07±31.7^a	88.33±3.31^ab	10.55±2.07^a	1.91±0.11^ab
300	38.97±2.15^a	766.36±32.2^a	96.67±2.07^a	11.27±1.98^a	1.65±0.23^b
400	30.97±1.98^b	588.12±20.7^b	83.33±5.54^b	9.10±2.13^b	2.08±0.18^a

牛磺酸添加量/(mg/kg)	终末体质量/g	增重率/%	成活率/%	出肉率/%	饵料系数
0	30.65±1.53^b	581.23±28.7^b	78.33±4.83^b	8.92±0.71^b	2.12±0.13^a
100	32.71±2.03^ab	626.43±19.1^ab	85.00±4.87^ab	9.13±1.21^b	2.07±0.21^a
200	35.81±1.87^a	681.07±31.7^a	88.33±3.31^ab	10.55±2.07^a	1.91±0.11^ab
300	38.97±2.15^a	766.36±32.2^a	96.67±2.07^a	11.27±1.98^a	1.65±0.23^b
400	30.97±1.98^b	588.12±20.7^b	83.33±5.54^b	9.10±2.13^b	2.08±0.18^a

牛磺酸添加量/(mg/kg)	溶菌酶/(U/mL)	总蛋白/(g/L)	谷丙转氨酶/(U/L)	谷草转氨酶/(U/L)
0	2.12±0.12^b	109.56±4.23^b	15.65±1.21^a	18.32±0.54^a
100	2.18±0.11^b	113.43±5.12^b	14.23±1.13^a	16.67±0.66^a
200	3.12±0.22^a	125.26±4.34^a	9.45±0.78^b	8.43±0.87^b
300	3.45±0.28^a	130.14±3.21^a	7.45±0.98^b	7.34±0.65^b
400	2.68±0.15^ab	110.43±2.31^b	14.21±1.12^a	16.23±0.87^a